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W3110: 4150236..4151456<br /><gbrowseImage>
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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10066 ECOCYC:EG10066]
EcoCyc:EG10066
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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EcoGene:EG10066
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
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[http://ecogene.org/geneinfo.php?eg_id=EG10066 EcoGene:EG10066]
RegulonDB:ECK120000062
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
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EchoBASE:EB0064
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0010976
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Latest revision as of 20:47, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

argD

Mnemonic

Arginine

Synonyms

ECK3347, b3359, JW3322, Arg1, dapC, dtu[1], alaB, argG, dtu

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

75.16 minutes 

MG1655: 3488202..3486982
<gbrowseImage> name=NC_000913:3486982..3488202 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3418085..3416865
<gbrowseImage> name=NC_012967:3416865..3418085 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3374139..3375359
<gbrowseImage> name=NC_012759:3374139..3375359 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4150236..4151456
<gbrowseImage> name=NC_007779:4150236..4151456 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3585947..3584727
<gbrowseImage> name=NC_010473:3584727..3585947 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3486985

Edman degradation

PMID:9298646[2]
PMID:10074354[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔargD (Keio:JW3322)

deletion

deletion

Auxotrophies

PMID:16738554[4]

Shigen

CGSC10482[5]

argD::Tn5KAN-I-SceI (FB21129)

Insertion at nt 429 in Plus orientation

Auxotrophies

PMID:15262929[6]

E. coli Genome Project:FB21129

does not contain pKD46

argD::Tn5KAN-I-SceI (FB21130)

Insertion at nt 429 in Plus orientation

Auxotrophies

PMID:15262929[6]

E. coli Genome Project:FB21130

contains pKD46

argD37

Auxotrophies

PMID:4872548[7]

CGSC:5152

argD85

Auxotrophies

CGSC:5201

ΔargD767::kan

deletion

deletion

Auxotrophies

PMID:16738554[4]

CGSC:100042

argD767(del)::FRTKanFRT

Mutagenesis rate

Decreased stress-induced mutagenesis (SIM) phenotype.

PMID:23224554[8]

Parental Strain: SMR4562

Experimental Strain: SMR12005

Mutation was comparatively weak with decrease only happening to 33-67% of population.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3322

Plasmid clone

Shigen

PMID:16769691[9]

Status:Clone OK

Primer 1:GCCGCAATTGAACAAACAGCAAT

Primer 2:CCgGCCCCAACCACCTTCGCCAC

15C3

Kohara Phage

Genobase

PMID:3038334[10]

4C5

Kohara Phage

Genobase

PMID:3038334[10]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[5]

est. P1 cotransduction: 5% [11]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[5]

est. P1 cotransduction: 57% [11]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10066

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10066

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000062

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947864

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0064

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010976

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  3. ↑ Ledwidge, R & Blanchard, JS (1999) The dual biosynthetic capability of N-acetylornithine aminotransferase in arginine and lysine biosynthesis. Biochemistry 38 3019-24 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  7. ↑ Itikawa, H et al. (1968) Enzymic basis for a genetic suppression: accumulation and deacylation of N-acetylglutamic gamma-semialdehyde in enterobacterial mutants. Biochim. Biophys. Acta 159 547-50 PubMed
  8. ↑ Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
  9. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  10. ↑ 10.0 10.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  11. ↑ 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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