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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="L46a9e73ab4982"  class=" tableEdit Gene_allele_table dataTable"   
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11002 ECOCYC:EG11002]
EcoCyc:EG11002
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[EcoGene]]
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EcoGene:EG11002
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
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[http://ecogene.org/geneinfo.php?eg_id=EG11002 EcoGene:EG11002]
RegulonDB:ECK120000991
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[EchoBASE]]
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EchoBASE:EB0995
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0009268
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Latest revision as of 20:44, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

thyA

Mnemonic

Thymine

Synonyms

ECK2823, b2827, JW2795[1], JW2795

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

63.85 minutes 

MG1655: 2963177..2962383
<gbrowseImage> name=NC_000913:2962383..2963177 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2859775..2858981
<gbrowseImage> name=NC_012967:2858981..2859775 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2849531..2850325
<gbrowseImage> name=NC_012759:2849531..2850325 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2963811..2963017
<gbrowseImage> name=NC_007779:2963017..2963811 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3057047..3056253
<gbrowseImage> name=NC_010473:3056253..3057047 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2962383

Edman degradation

PMID:6308660[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

thyA(del) (Keio:JW2795)

deletion

deletion

PMID:16738554[3]

Shigen

thyA751

CGSC:4790

thyA25

CGSC:4870

thyA715

CGSC:5108

thyA36

CGSC:5214

thyA6

CGSC:5229

thyA47

CGSC:5247

thyA52

CGSC:5930

thyA12

CGSC:6324

thyA704

CGSC:6454

thyA3

CGSC:6456

thyA81

CGSC:6550

thyA82

CGSC:6553

thyA56

CGSC:6624

thyA117

CGSC:6693

thyA61

CGSC:6774

thyA144

CGSC:6965

thyA54

CGSC:6984

thyA42

CGSC:7046

thyA748::Tn10

CGSC:7146

thyA147

CGSC:7435

thyA148

CGSC:7441

thyA45

CGSC:7744

thyA702

CGSC:7782

thyA55

CGSC:8013

thyA43

CGSC:8047

thyA11

CGSC:8289

thyA24

CGSC:8317

thyA305

CGSC:8415

thyA14

CGSC:8453

thyA33

CGSC:8524

thyA114(Stable)::Mu

CGSC:8530

thyA20

CGSC:8680

thyA707

CGSC:8791

thyA111

CGSC:8970

thyA16

CGSC:9232

thyA15

CGSC:9246

thyA705

CGSC:9518

thyA48

CGSC:9636

thyA714

CGSC:9655

thyA95

CGSC:9679

thyA44

CGSC:9739

thyA710

CGSC:9829

thyA0

CGSC:9977

thyA299

CGSC:10047

thyA59

CGSC:10129

thyA746

CGSC:10313

thyA752(del)

CGSC:10675

thyA719

CGSC:10685

thyA755

CGSC:10854

thyA756

CGSC:10867

thyA57(del)

CGSC:10899

thyA758

CGSC:10937

thyA38

CGSC:11040

thyA26

CGSC:11309

thyA41

CGSC:11313

thyA23

CGSC:11395

thyA325

CGSC:11630

thyA46

CGSC:11771

thyA2

CGSC:13565

thyA18

CGSC:13591

thyA34

CGSC:13776

thyA10

CGSC:13932

thyA39

CGSC:13966

thyA29

CGSC:14002

thyA27

CGSC:14007

thyA53

CGSC:14054

thyA28

CGSC:14170

thyA30

CGSC:14178

thyA31

CGSC:14184

thyA49

CGSC:14623

thyA709

CGSC:14678

thyA17

CGSC:16221

thyA51

CGSC:16360

thyA321

CGSC:16724

thyA58

CGSC:16920

thyA50

CGSC:16936

thyA40

CGSC:17076

thyA333

CGSC:19205

thyA9999(ts)::Mud(Ap,lac)

temperature sensitive

CGSC:19260

thyA149

CGSC:19317

thyA101

CGSC:19651

thyA9

CGSC:23655

thyA326

CGSC:27048

thyA753

CGSC:29474

thyA281

CGSC:66486

thyA294

CGSC:72642

thyA119

CGSC:74742

thyA760

CGSC:79050

thyA749

CGSC:80478

thyA750

CGSC:80480


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2795

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAAACAGTATTTAGAACTGAT

Primer 2:CCGATAGCCACCGGCGCTTTAAT

3G11

Kohara Phage

Genobase

PMID:3038334[5]

5A10

Kohara Phage

Genobase

PMID:3038334[5]

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[6]

est. P1 cotransduction: 67% [7]

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[6]

est. P1 cotransduction: % [7]
Synonyms:zgd-210::Tn10, zgf-210::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11002

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11002

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000991

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:949035

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0995

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009268

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Belfort, M et al. (1983) Primary structure of the Escherichia coli thyA gene and its thymidylate synthase product. Proc. Natl. Acad. Sci. U.S.A. 80 4914-8 PubMed
  3. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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