ftsA:Expression: Difference between revisions

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deleting everything from the molecules per cell table on the expression pages
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="G49c026ea3c859"  class=" tableEdit MoleculesPerCellTable"   
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!|Molecule!!Organism or Strain!!Value!!Units!!Experimental Conditions!!Assay used!!Notes!!Reference(s)
!|Molecule!!Organism or Strain!!Value!!Units!!Experimental Conditions!!Assay used!!Notes!!Reference(s)
|-
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Protein
|
''E. coli'' D1210
|
175
|
molecules/cell
|
*Medium:  LB broth
*Temperature (°C):  37
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 1.67
*Growth phase: other:
|
quantitative western
|
|
PMID:1406287
|-
|
Protein
|
''E. coli'' D1210
|
200
|
molecules/cell
|
*Medium:  LB + 0.4% Glucose
*Temperature (°C):  37
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 2.00
*Growth phase: other:
|
quantitative western
|
|
PMID:1406287
|-
|
Protein
|
''E. coli'' D1210
|
127
|
molecules/cell
|
*Medium:  M9 + 1% casamino acids + 0.4% glucose
*Temperature (°C):  37
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 1.62
*Growth phase: other:
|
quantitative western
|
|
PMID:1406287
|-
|
Protein
|
''E. coli'' D1210
|
100
|
molecules/cell
|
*Medium:  M9 + 1% casamino acids + 0.4% glycerol
*Temperature (°C):  37
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 1.03
*Growth phase: other:
|
quantitative western
|
|
PMID:1406287
|-
|
Protein
|
''E. coli'' D1210
|
74
|
molecules/cell
|
*Medium:  M9 + 0.4% glucose
*Temperature (°C):  37
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 0.52
*Growth phase: other:
|
quantitative western
|
|
PMID:1406287
|-
|
Protein
|
''E. coli'' D1210
|
48
|
molecules/cell
|
*Medium:  M9 + 0.4% glycerol
*Temperature (°C):  37
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 0.48
*Growth phase: other:
|
quantitative western
|
|
PMID:1406287
|-
|
Protein
|
''E. coli'' D1210
|
45
|
molecules/cell
|
*Medium:  M9 + 0.4% succinate
*Temperature (°C):  37
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 0.44
*Growth phase: other:
|
quantitative western
|
|
PMID:1406287
|-
|
Protein
|
''E. coli'' D1210
|
43
|
molecules/cell
|
*Medium:  M9 + 0.4% acetate
*Temperature (°C):  37
*Doubling time (min): Growth Rate(min):  Doubling time (per hour) = 0.41
*Growth phase: other:
|
quantitative western
|
|
PMID:1406287
|-
|
Protein
|
Ecoli K-12
|
58.922+/-0.223
|
Molecules/cell
|
*Medium: 
*Temperature (°C): 
*Doubling time (min): Growth Rate(min):  *
*Growth phase: other:  *
|
|
Single Molecule Fluorescence
|
PMID:20671182
|-
|
mRNA
|
Ecoli K-12
|
0.161648177
|
Molecules/cell
|
*Medium: 
*Temperature (°C): 
*Doubling time (min): Growth Rate(min):  *
*Growth phase: other:  *
|
|
by RNA_Seq
|
PMID:20671182
|-
|
Protein
|
''E. coli'' K-12 MG1655
|
984
|
molecules/cell/generation
|
*Medium: MOPS Complete 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
|
PMID: 24766808
|-
|
Protein
|
''E. coli'' K-12 MG1655
|
575
|
molecules/cell/generation
|
*Medium: MOPS Minimal 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
|
PMID: 24766808
|-
|
Protein
|
''E. coli'' K-12 MG1655
|
1013
|
molecules/cell/generation
|
*Medium: MOPS Complete without Methionine 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
|
PMID: 24766808


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<!--box uid=421aa90e079fa326b6494f812ad13e79.32723.S46ab372eaa892-->||{{RegulonDBfig|ECK120000333.jpg|FTSA}}
<!--box uid=421aa90e079fa326b6494f812ad13e79.32723.S46ab372eaa892-->||{{RegulonDBfig|ECK120000333.jpg|ECK120000333}}
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===Notes===
===Notes===
{{Expression_transcription_placeholder}}
{{Expression_transcription_placeholder}}
Overproduction of FtsA causes long non-septate filaments to form and inhibits normal cell septation. <ref name='PMID:2198274'/>
Overproduction does block division but transcription from cloned fts promoters is not further repressed. <ref name='PMID:2515414'/>
RcsB effects the efficiency of the transcription of ''ftsA1p''. Specifically, causing it too increase in efficiency  as more of RcsB is added. <ref name='PMID:10564486'/>


==Translation and Regulation of Translation==
==Translation and Regulation of Translation==
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name=NC_000913:103962..104002
name=NC_000913:103962..104002
source=ecoli
source=MG1655
type=Gene+DNA_+Protein
type=Gene+DNA_+Protein
preset=Nterminus
preset=Nterminus
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microarray  
microarray
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[http://genexpdb.ou.edu/index.php?query=b0094 GenExpDB:b0094] ([[EcoliGenExpDB|EcoliWiki Page]])  
[http://genexpdb.ou.edu/index.php?query=b0094 GenExpDB:b0094] ([[EcoliGenExpDB|EcoliWiki Page]])  
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="P46ab3730b7e6e"  class=" tableEdit Gene_accessions_table"   
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10339 EcoCyc:EG10339]
EcoCyc:EG10339
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EchoBASE]]
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0335 EchoBASE:EB0335]
EchoBASE:EB0335
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EcoGene]]
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EcoGene:EG10339
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[http://ecogene.org/geneinfo.php?eg_id=EG10339 EcoGene:EG10339]
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[[RegulonDB]]
|
RegulonDB:ECK120000333
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
|
ASAP:ABE-0000331
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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!|Name!!URL!!Comments
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Latest revision as of 19:38, 21 July 2015

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Overview Transcription Translation Turnover Experimental Links References Suggestions

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

[back to top]


See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli D1210

175

molecules/cell

  • Medium: LB broth
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 1.67
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

200

molecules/cell

  • Medium: LB + 0.4% Glucose
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 2.00
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

127

molecules/cell

  • Medium: M9 + 1% casamino acids + 0.4% glucose
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 1.62
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

100

molecules/cell

  • Medium: M9 + 1% casamino acids + 0.4% glycerol
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 1.03
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

74

molecules/cell

  • Medium: M9 + 0.4% glucose
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 0.52
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

48

molecules/cell

  • Medium: M9 + 0.4% glycerol
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 0.48
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

45

molecules/cell

  • Medium: M9 + 0.4% succinate
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 0.44
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

E. coli D1210

43

molecules/cell

  • Medium: M9 + 0.4% acetate
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min): Doubling time (per hour) = 0.41
  • Growth phase: other:

quantitative western

PMID:1406287[1]

Protein

Ecoli K-12

58.922+/-0.223

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182[2]

mRNA

Ecoli K-12

0.161648177

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182[2]

Protein

E. coli K-12 MG1655

984

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[3]

Protein

E. coli K-12 MG1655

575

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[3]

Protein

E. coli K-12 MG1655

1013

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[3]

edit table

Notes

Transcription and Transcriptional Regulation

[back to top]


See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ftsAZ

mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ

ftsQAZ

edit table
Figure courtesy of RegulonDB


Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Overproduction of FtsA causes long non-septate filaments to form and inhibits normal cell septation. [4]

Overproduction does block division but transcription from cloned fts promoters is not further repressed. [5]

RcsB effects the efficiency of the transcription of ftsA1p. Specifically, causing it too increase in efficiency as more of RcsB is added. [6]

Translation and Regulation of Translation

[back to top]


<gbrowseImage> name=NC_000913:103962..104002 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.


Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
edit table


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0094 (EcoliWiki Page)

NCBI GEO profiles for ftsA

microarray

GenExpDB:b0094 (EcoliWiki Page)

Summary of data for ftsA from multiple microarray studies

edit table


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
edit table

<protect>

Notes

Accessions Related to ftsA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10339

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0335

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0094

EcoGene

EcoGene:EG10339

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000333

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000331

Escherichia coli str. K-12 substr. MG1655

edit table


Notes

Links

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References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Wang, H & Gayda, RC (1992) Quantitative determination of FtsA at different growth rates in Escherichia coli using monoclonal antibodies. Mol. Microbiol. 6 2517-24 PubMed
  2. ↑ 2.0 2.1 Taniguchi, Y et al. (2010) Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 329 533-8 PubMed
  3. ↑ 3.0 3.1 3.2 Li, GW et al. (2014) Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources. Cell 157 624-35 PubMed
  4. ↑ Wang, HC & Gayda, RC (1990) High-level expression of the FtsA protein inhibits cell septation in Escherichia coli K-12. J. Bacteriol. 172 4736-40 PubMed
  5. ↑ Dewar, SJ et al. (1989) Transcriptional regulation of cell division genes in Escherichia coli. Mol. Microbiol. 3 1371-7 PubMed
  6. ↑ Carballès, F et al. (1999) Regulation of Escherichia coli cell division genes ftsA and ftsZ by the two-component system rcsC-rcsB. Mol. Microbiol. 34 442-50 PubMed

Categories

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