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W3110: 4256606..4257478<br /><gbrowseImage>
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The mutation conferred a decrease in strong reduction in SIM with mutant frequency being reduced by 90 percent of the wild type. See table S3 for full experimental results.   
The mutation conferred a decrease in strong reduction in SIM with mutant frequency being reduced by 90 percent of the wild type. See table S3 for full experimental results.   
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''ubiA::Tn''10''dCam
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Insertion
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SDS-EDTA Sensitivity
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Increase in SDS-EDTA sensitivity
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PMID:23224554
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Parent Strain: SMR4562 Experimental Strain: SMR12311
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The mutation conferred an an increase in SDS-EDTA sensitivity relative to the wild type. See table S7 for a summary of results. 
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''ubiA::Tn''10''dCam
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Insertion
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Increased sensitivity toward UV radiation
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PMID:23224554
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Parent Strain: SMR4562 Experimental Strain: SMR12311
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The mutation conferred an an increase in UV sensitivity relative to the wild type. See table S7 for a review of experimental results. 
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SMR4562 yiaG-yfp FRT ''ubiA''::Tn''10''dCam
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Insertion
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Sigma <sup>S</sup> activity
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Decrease in Sigma<sup>S</sup> activity
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PMID:23224554
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Parental Strain: SMR10582
Experimental Strain: SMR14447
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See table S8 for full experimental results. 
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CAG45114 ''ubiA''::Tn''10''dCam
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Insertion
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Sigma<sup>E</sup> activity
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Decrease in sigma<sup>E</sup> activity
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PMID:23224554
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Parental Strain: CAG45114
Experimental Strain: SMR15321
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See table S11 for full experimental data. 


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!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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EcoCyc:EG11370
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[[EcoGene]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11370 ECOCYC:EG11370]
EcoGene:EG11370
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
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[http://ecogene.org/geneinfo.php?eg_id=EG11370 EcoGene:EG11370]
RegulonDB:ECK120001338
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[EchoBASE]]
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EchoBASE:EB1344
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0013229
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Latest revision as of 20:51, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ubiA

Mnemonic

Ubiquinone

Synonyms

ECK4032, b4040, JW4000, cyr, sdgG?[1][2], sdgG?

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.62 minutes 

MG1655: 4251039..4251911
<gbrowseImage> name=NC_000913:4251039..4251911 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4231948..4232820
<gbrowseImage> name=NC_012967:4231948..4232820 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4189884..4190756
<gbrowseImage> name=NC_012759:4189884..4190756 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4256606..4257478
<gbrowseImage> name=NC_007779:4256606..4257478 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4350735..4351607
<gbrowseImage> name=NC_010473:4350735..4351607 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ubiA::Tn10dCam

Insertion

Mutagenesis rate

Decrease in the rate of Stress Induced Mutagenesis (SIM).

PMID:23224554[3]

Parent Strain: SMR4562 Experimental Strain: SMR12311

The mutation conferred a decrease in strong reduction in SIM with mutant frequency being reduced by 90 percent of the wild type. See table S3 for full experimental results.

ubiA::Tn10dCam

Insertion

SDS-EDTA Sensitivity

Increase in SDS-EDTA sensitivity

PMID:23224554[3]

Parent Strain: SMR4562 Experimental Strain: SMR12311

The mutation conferred an an increase in SDS-EDTA sensitivity relative to the wild type. See table S7 for a summary of results.

ubiA::Tn10dCam

Insertion

Increased sensitivity toward UV radiation

PMID:23224554[3]

Parent Strain: SMR4562 Experimental Strain: SMR12311

The mutation conferred an an increase in UV sensitivity relative to the wild type. See table S7 for a review of experimental results.

SMR4562 yiaG-yfp FRT ubiA::Tn10dCam

Insertion

Sigma S activity

Decrease in SigmaS activity

PMID:23224554[3]

Parental Strain: SMR10582 Experimental Strain: SMR14447

See table S8 for full experimental results.

CAG45114 ubiA::Tn10dCam

Insertion

SigmaE activity

Decrease in sigmaE activity

PMID:23224554[3]

Parental Strain: CAG45114 Experimental Strain: SMR15321

See table S11 for full experimental data.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4000

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGAGTGGAGTCTGACGCAGAA

Primer 2:CCGAAATGCCAGTAACTCATTGC

1F8

Kohara Phage

Genobase

PMID:3038334[5]

12B4

Kohara Phage

Genobase

PMID:3038334[5]

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[6]

est. P1 cotransduction: 70% [7]

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[6]

est. P1 cotransduction: 7% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11370

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11370

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001338

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948540

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1344

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013229

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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