ubiA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ubiA

Mnemonic

Ubiquinone

Synonyms

ECK4032, b4040, JW4000, cyr, sdgG?[1][2], sdgG?

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.62 minutes 

MG1655: 4251039..4251911
<gbrowseImage> name=NC_000913:4251039..4251911 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4231948..4232820
<gbrowseImage> name=NC_012967:4231948..4232820 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4189884..4190756
<gbrowseImage> name=NC_012759:4189884..4190756 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4256606..4257478
<gbrowseImage> name=NC_007779:4256606..4257478 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4350735..4351607
<gbrowseImage> name=NC_010473:4350735..4351607 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ubiA::Tn10dCam

Insertion

Mutagenesis rate

Decrease in the rate of Stress Induced Mutagenesis (SIM).

PMID:23224554[3]

Parent Strain: SMR4562 Experimental Strain: SMR12311

The mutation conferred a decrease in strong reduction in SIM with mutant frequency being reduced by 90 percent of the wild type. See table S3 for full experimental results.

ubiA::Tn10dCam

Insertion

SDS-EDTA Sensitivity

Increase in SDS-EDTA sensitivity

PMID:23224554[3]

Parent Strain: SMR4562 Experimental Strain: SMR12311

The mutation conferred an an increase in SDS-EDTA sensitivity relative to the wild type. See table S7 for a summary of results.

ubiA::Tn10dCam

Insertion

Increased sensitivity toward UV radiation

PMID:23224554[3]

Parent Strain: SMR4562 Experimental Strain: SMR12311

The mutation conferred an an increase in UV sensitivity relative to the wild type. See table S7 for a review of experimental results.

SMR4562 yiaG-yfp FRT ubiA::Tn10dCam

Insertion

Sigma S activity

Decrease in SigmaS activity

PMID:23224554[3]

Parental Strain: SMR10582 Experimental Strain: SMR14447

See table S8 for full experimental results.

CAG45114 ubiA::Tn10dCam

Insertion

SigmaE activity

Decrease in sigmaE activity

PMID:23224554[3]

Parental Strain: CAG45114 Experimental Strain: SMR15321

See table S11 for full experimental data.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4000

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGAGTGGAGTCTGACGCAGAA

Primer 2:CCGAAATGCCAGTAACTCATTGC

1F8

Kohara Phage

Genobase

PMID:3038334[5]

12B4

Kohara Phage

Genobase

PMID:3038334[5]

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[6]

est. P1 cotransduction: 70% [7]

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[6]

est. P1 cotransduction: 7% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11370

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11370

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001338

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948540

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1344

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013229

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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