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|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK2308, b2314, JW5378''<ref name='LIB:Riley_2006'/>
''ECK2308, b2314, JW5378''<ref name='LIB:Riley_2006'/>, JW5378''


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*  
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****************************************************************************************** -->{{Gene_location_help}}
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{|   id="L46a9db2564565"  class=" tableEdit Gene_location_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="L46a9db2564565"  class=" tableEdit Gene_location_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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52.35 minutes&nbsp;
52.35 minutes&nbsp;
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MG1655: 2429706..2429044<br />{{GBrowseFigureByCoord|2429044|2429706|ecoli|MG1655}}
MG1655: 2429706..2429044<br /><gbrowseImage>
name=NC_000913:2429044..2429706
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
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*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10218 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10218 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|2428044|2430706|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|2428044|2430706|GBrowse(MG1655)}}
   
   
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{{GBrowseFastaLink|ecoli|NC_000913|2429044|2429706|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|2429044|2429706|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10218 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10218 EcoCyc Sequence Dump]  
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NC_012967: 2375276..2374614<br />{{GBrowseFigureByCoord|2374614|2375276|REL606|NC_012967}}
NC_012967: 2375276..2374614<br /><gbrowseImage>
name=NC_012967:2374614..2375276
source=REL606
preset=GeneLocation
</gbrowseImage>
 
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{{GBrowseLink|REL606|NC_012967|2374614|2375276|GBrowse (REL606)}}
{{GBrowseLink|REL606|NC_012967|2374614|2375276|GBrowse (REL606)}}
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NC_012759: 2314849..2315511<br />{{GBrowseFigureByCoord|2314849|2315511|BW2952|NC_012759}}
NC_012759: 2314849..2315511<br /><gbrowseImage>
name=NC_012759:2314849..2315511
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
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{{GBrowseLink|BW2952|012759|2314849|NC_2315511|GBrowse (BW2952)}}
{{GBrowseLink|BW2952|NC_012759|2314849|2315511|GBrowse (BW2952)}}
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{{GBrowseFastaLink|BW2952|012759|2314849|NC_2315511|FASTA Dump}}  
{{GBrowseFastaLink|BW2952|NC_012759|2314849|2315511|FASTA Dump}}  
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&nbsp;
&nbsp;
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W3110: 2437130..2436468<br />{{GBrowseFigureByCoord|2436468|2437130|ecoli_W3110|W3110}}
W3110: 2437130..2436468<br /><gbrowseImage>
name=NC_007779:2436468..2437130
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
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{{GBrowseLink|ecoli_W3110|AC_000091|2435468|2438130|GBrowse(W3110)}}  
{{GBrowseLink|W3110|NC_007779|2435468|2438130|GBrowse(W3110)}}  
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|2436468|2437130|FASTA Dump}}  
{{GBrowseFastaLink|W3110|NC_007779|2436468|2437130|FASTA Dump}}  
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DH10B: 2521471..2520809<br />{{GBrowseFigureByCoord|2520809|2521471|ecoli_DH10B|DH10B}}
DH10B: 2521471..2520809<br /><gbrowseImage>
name=NC_010473:2520809..2521471
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
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{{GBrowseLink|ecoli_DH10B|NC_010473|2519485|2522795|GBrowse(DH10B)}}
{{GBrowseLink|DH10B|NC_010473|2519485|2522795|GBrowse(DH10B)}}
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{{GBrowseFastaLink|ecoli_DH10B|NC_010473|2519485|2522795|FASTA Dump}}  
{{GBrowseFastaLink|DH10B|NC_010473|2519485|2522795|FASTA Dump}}  


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****************************************************************************************** -->{{Gene_sequence_features_help}}
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{|   id="H46a9db261b361"  class=" tableEdit Gene_sequence_features_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="H46a9db261b361"  class=" tableEdit Gene_sequence_features_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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*  
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****************************************************************************************** -->{{Gene_alleles_help}}
****************************************************************************************** -->{{Gene_alleles_help}}
{| {{Prettytable}} id="N46a9db266098d"  class=" tableEdit Gene_allele_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="N46a9db266098d"  class=" tableEdit Gene_allele_table dataTable"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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|-  
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0007643 dedD]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0007643 dedD::Tn5KAN-2] (FB20761)
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Insertion at nt 407 in Plus orientation
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PMID:12519969
PMID:15262929
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[[asapstrains:ASAP|ASAP]]
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20761]
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At position 407 in Plus orientation, contains plasmid  [[pKD46]].<br />ASAP strain name: FB20761
contains [[pKD46]]  
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dedD
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deletion
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Sensitivity to
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decreases sensitivity to bicyclomycin
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PMID:21357484
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fig 2


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===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.15191.Z4e04b9af4aa39-->
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Z4e04b9af4aa39"  class=" tableEdit Genetic_interaction_table dataTable" 
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.15191.Z4e04b9af4aa39&page=15191&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || ||
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<!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.15191.Z4e04b9af4aa39--></protect>
<!-- Genetic Interacdtions Notes -->
===Notes===


==Genetic Resources==
==Genetic Resources==
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{|   id="R46a9db27180e2"  class=" tableEdit Gene_resource_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="R46a9db27180e2"  class=" tableEdit Gene_resource_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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Primer 2:CCATTCGGCGTATAGCCCATTAC  
Primer 2:CCATTCGGCGTATAGCCCATTAC  
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[[:Category:Kohara_Phage:9D2|9D2]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=406 Genobase]
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PMID:3038334
|-
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[[:Category:Kohara_Phage:12F12|12F12]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=407 Genobase]
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PMID:3038334
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{|   id="M46a9db27ac927"  class=" tableEdit Gene_accessions_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="M46a9db27ac927"  class=" tableEdit Gene_accessions_table"   
|- {{table_heading_style}}
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10218 EcoCyc:EG10218]
EcoCyc:EG10218
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EcoGene]]
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0214 EchoBASE:EB0214]
EcoGene:EG10218
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[RegulonDB]]
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[http://chase.ou.edu/macro/searchlink.php?bnum=b2314 EcoliGenExpDB:b2314]
RegulonDB:ECK120000212
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C2859 ECOL199310CYC:C2859]
[http://www.ncbi.nlm.nih.gov/gene?term=944971 GeneID:944971]
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Escherichia coli str. K-12 substr. MG1655
|-  
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EchoBASE]]
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[http://ecogene.org/geneinfo.php?eg_id=EG10218 EcoGene:EG10218]
EchoBASE:EB0214
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[ASAP]]
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[http://BioCyc.org/ECOO157/new-image?object=DEDD ECOO157CYC:DEDD]
ASAP:ABE-0007643
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
|-  
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0007643&formSubmitReturn=1 ASAP:ABE-0007643]
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{|   id="K46a6f8440de6d"  class=" tableEdit Links_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46a6f8440de6d"  class=" tableEdit Links_table"   
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!|Name!!URL!!Comments
!|Name!!URL!!Comments

Latest revision as of 20:41, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dedD

Mnemonic

Downstream E. coli DNA

Synonyms

ECK2308, b2314, JW5378[1], JW5378

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

52.35 minutes 

MG1655: 2429706..2429044
<gbrowseImage> name=NC_000913:2429044..2429706 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2375276..2374614
<gbrowseImage> name=NC_012967:2374614..2375276 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2314849..2315511
<gbrowseImage> name=NC_012759:2314849..2315511 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2437130..2436468
<gbrowseImage> name=NC_007779:2436468..2437130 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2521471..2520809
<gbrowseImage> name=NC_010473:2520809..2521471 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔdedD (Keio:JW5378)

deletion

deletion

PMID:16738554[2]

Shigen

dedD::Tn5KAN-2 (FB20761)

Insertion at nt 407 in Plus orientation

PMID:15262929[3]

E. coli Genome Project:FB20761

contains pKD46

ΔdedD::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[4]

dedD

deletion

Sensitivity to

decreases sensitivity to bicyclomycin

PMID:21357484[5]

fig 2

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5378

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGCAAGTAAGTTTCAGAATCG

Primer 2:CCATTCGGCGTATAGCCCATTAC

9D2

Kohara Phage

Genobase

PMID:3038334[7]

12F12

Kohara Phage

Genobase

PMID:3038334[7]

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[8]

est. P1 cotransduction: 19% [9]
Synonyms:zej-223::Tn10

zfd-1::Tn10

Linked marker

CAG18467 = CGSC7408[8]

est. P1 cotransduction: 3% [9]
Synonyms:zfb-1::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10218

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10218

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000212

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944971

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0214

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007643

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  4. ↑ Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  5. ↑ Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 CGSC: The Coli Genetics Stock Center
  9. ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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