amn:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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{|{{Prettytable}}
{|   id="S46a9d367530df"  class=" tableEdit Gene_nomenclature_table" 
 
|-
!align=left {{table_heading_style}} |Standard name
||
''amn''
|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Mnemonic
|
||
''amn''
AMP nucleosidase
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK1977, b1982, JW1963''<ref name='LIB:Riley_2006'/>, JW1963''


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!{{table_heading_style}} |Synonyms
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.14529.S46a9d367530df&page=14529&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> ||
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''ECK1977, b1982, JW1963''<ref name='LIB:Riley_2006'/>
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===Notes===
===Notes===
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{|class = 'sortable' {{Prettytable}} id='136789'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="U46a9d367cf4ac"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|-
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[[BW2952]]
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|
NC_012759: 1945568..1947022<br /><gbrowseImage>
name=NC_012759:1945568..1947022
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|1945568|1947022|GBrowse (BW2952)}}
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{{GBrowseFastaLink|BW2952|NC_012759|1945568|1947022|FASTA Dump}}
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MG1655
MG1655
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44.25 minutes&nbsp;
44.25 minutes&nbsp;
|
|
MG1655: 2053085..2054539<br />{{GBrowseFigureByCoord|2052085|2055539|ecoli|MG1655}}
MG1655: 2053085..2054539<br /><gbrowseImage>
name=NC_000913:2053085..2054539
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
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*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10039 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10039 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:2052085..2055539 GBrowse(MG1655)]
{{GBrowseLink|MG1655|NC_000913|2052085|2055539|GBrowse(MG1655)}}
   
   
|
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*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:2053085..2054539&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|2053085|2054539|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10039 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10039 EcoCyc Sequence Dump]  
|-
|-
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[[REL606]]
|
 
|
NC_012967: 1993060..1994514<br /><gbrowseImage>
name=NC_012967:1993060..1994514
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|1993060|1994514|GBrowse (REL606)}}
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{{GBrowseFastaLink|REL606|NC_012967|1993060|1994514|FASTA Dump}}
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W3110
W3110
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&nbsp;
&nbsp;
|
|
W3110: 2057198..2058652<br />{{GBrowseFigureByCoord|2056198|2059652|ecoli_W3110|W3110}}
W3110: 2057198..2058652<br /><gbrowseImage>
name=NC_007779:2057198..2058652
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
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*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:2056198..2059652 GBrowse(W3110)]
{{GBrowseLink|W3110|NC_007779|2056198|2059652|GBrowse(W3110)}}
|
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*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:2057198..2058652&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|W3110|NC_007779|2057198|2058652|FASTA Dump}}
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[[DH10B]]
|
 
|
DH10B: 2144093..2145547<br /><gbrowseImage>
name=NC_010473:2144093..2145547
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|DH10B|NC_010473|2141185|2148455|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|2141185|2148455|FASTA Dump}}
 
|- class="tableEdit_footer"
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===Notes===
===Notes===
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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Coding Start (SO:0000323)
|
[[MG1655]]
|
2053085
|
Edman degradation
|
PMID:2690948<br />
|
 
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<protect><!--Sequence_notes--></protect>
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====Notes====
====Notes====
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{|class = 'sortable' {{Prettytable}} id='136791'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Q46a9d368c75ff"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
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!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
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&Delta;amn (Keio:JW1963)
&Delta;amn (Keio:JW1963)
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[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107909 CGSC9628]<ref name='LIB:cgsc'/>
|
|-
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0006579 amn::Tn5KAN-2] (FB20556)
|
Insertion at nt 239 in Minus orientation
|
 
|
 
|
 
|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20556]
|
contains [[pKD46]]
|-
|
''&#916;amn-756::kan''
|
 
|
 
|
 
|
 
|
PMID:16738554
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
[[cgscmutation:99996|CGSC:99996]]
|
|
   
   
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{{Gene_alleles_help}}
 
<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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===Notes===


==Genetic Resources==
==Genetic Resources==
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JW1963
JW1963
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
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[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:Clone OK
Status:Clone OK
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Primer 2:CCTCGGAACGGCGGCTCATTAAA  
Primer 2:CCTCGGAACGGCGGCTCATTAAA  
|-
|-
|
[[:Category:Kohara_Phage:7D4|7D4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=346 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:9B2|9B2]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=347 Genobase]
|
PMID:3038334
|-  
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[[yedZ:Gene|zed-3069::Tn10]]
[[yedZ:Gene|zed-3069::Tn10]]
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|
|
est. P1 cotransduction: 56% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 56% <ref name='LIB:Nichols_linked_Tn'/>  
|-
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|
|
zef-3129::Tn10
zef-3129::Tn10
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CAG12099 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39996 CGSC7397]<ref name='LIB:cgsc'/>
CAG12099 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39996 CGSC7397]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: 31% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 31% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:zee-3129::Tn10


Synonyms:zee-3129::Tn10
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===Notes===
===Notes===
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-  
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10039 EcoCyc:EG10039] 
|
|-
|
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0037 EchoBASE:EB0037] 
EcoCyc:EG10039
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b1982 EcoliGenExpDB:b1982] 
EcoGene:EG10039
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C2444 ECOL199310CYC:C2444] 
RegulonDB:ECK120000035
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG10039 EcoGene:EG10039]
[http://www.ncbi.nlm.nih.gov/gene?term=946508 GeneID:946508]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://BioCyc.org/ECOO157/new-image?object=AMN ECOO157CYC:AMN] 
EchoBASE:EB0037
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[ASAP]]
|
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/17764 CGSC:17764] 
ASAP:ABE-0006579
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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===Notes===
===Notes===
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===Categories===
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:MG1655]]
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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:amn genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:40, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

amn

Mnemonic

AMP nucleosidase

Synonyms

ECK1977, b1982, JW1963[1], JW1963


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

BW2952

NC_012759: 1945568..1947022
<gbrowseImage> name=NC_012759:1945568..1947022 source=BW2952 preset=GeneLocation </gbrowseImage>

MG1655

44.25 minutes 

MG1655: 2053085..2054539
<gbrowseImage> name=NC_000913:2053085..2054539 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1993060..1994514
<gbrowseImage> name=NC_012967:1993060..1994514 source=REL606 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2057198..2058652
<gbrowseImage> name=NC_007779:2057198..2058652 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2144093..2145547
<gbrowseImage> name=NC_010473:2144093..2145547 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2053085

Edman degradation

PMID:2690948[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δamn (Keio:JW1963)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9628[4]

amn::Tn5KAN-2 (FB20556)

Insertion at nt 239 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20556

contains pKD46

Δamn-756::kan

PMID:16738554[3]

CGSC:99996



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1963

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAATAATAAGGGCTCCGGTCT

Primer 2:CCTCGGAACGGCGGCTCATTAAA

7D4

Kohara Phage

Genobase

PMID:3038334[7]

9B2

Kohara Phage

Genobase

PMID:3038334[7]

zed-3069::Tn10

Linked marker

CAG18451 = CGSC7395[4]

est. P1 cotransduction: 56% [8]

zef-3129::Tn10

Linked marker

CAG12099 = CGSC7397[4]

est. P1 cotransduction: 31% [8]
Synonyms:zee-3129::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10039

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10039

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000035

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946508

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0037

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006579

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Leung, HB et al. (1989) Structure and regulation of the AMP nucleosidase gene (amn) from Escherichia coli. Biochemistry 28 8726-33 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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