znuC:Gene: Difference between revisions

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!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK1859, b1858, JW1847, yebM''<ref name='LIB:Riley_2006'/>
''ECK1859, b1858, JW1847, yebM''<ref name='LIB:Riley_2006'/>, yebM''


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****************************************************************************************** -->{{Gene_location_help}}
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{|   id="Z46a9d09ab6eea"  class=" tableEdit Gene_location_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Z46a9d09ab6eea"  class=" tableEdit Gene_location_table"   
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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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W3110: 1944376..1945131<br /><gbrowseImage>
W3110: 1944376..1945131<br /><gbrowseImage>
name=AC_000091:1944376..1945131
name=NC_007779:1944376..1945131
source=W3110
source=W3110
preset=GeneLocation
preset=GeneLocation
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{{GBrowseLink|W3110|AC_000091|1943376|1946131|GBrowse(W3110)}}  
{{GBrowseLink|W3110|NC_007779|1943376|1946131|GBrowse(W3110)}}  
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{{GBrowseFastaLink|W3110|AC_000091|1944376|1945131|FASTA Dump}}  
{{GBrowseFastaLink|W3110|NC_007779|1944376|1945131|FASTA Dump}}  
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{|   id="W46a9d09b6a056"  class=" tableEdit Gene_sequence_features_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="W46a9d09b6a056"  class=" tableEdit Gene_sequence_features_table"   
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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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{| {{Prettytable}} id="C46a9d09badd80"  class=" tableEdit Gene_allele_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C46a9d09badd80"  class=" tableEdit Gene_allele_table dataTable"   
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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&Delta;znuC (Keio:JW1847)
''znuC(del)'' (Keio:JW1847)
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deletion
deletion
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PMID:16738554
PMID:16738554
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107822 CGSC9541]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br />[http://cgsc2.biology.yale.edu/Strain.php?ID=107822 CGSC9541]<ref name='LIB:cgsc'/>  
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''&#916;znuC783::kan''
''znuC783(del)''::kan
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''znuC783''(del)::FRT
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Mutagenesis rate
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Decreased stress-induced mutagenesis (SIM) phenotype.
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PMID:23224554
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Parental Strain: SMR4562
Experimental Strain: SMR12211
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Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population.
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CAG45114 ''znuC783(del)''::FRTKanFRT
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deletion
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Sigma<sup>E</sup> activity
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Decrease in sigma<sup>e</sup> activity
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PMID:23224554
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Parental Strain: CAG45114
Experimental Strain: SMR15291
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See table S11 for full experimental data. 


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{|   id="U4e04cd3417d1a"  class=" tableEdit Genetic_interaction_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="U4e04cd3417d1a"  class=" tableEdit Genetic_interaction_table dataTable"   
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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{|   id="V46a9d09c612e9"  class=" tableEdit Gene_resource_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="V46a9d09c612e9"  class=" tableEdit Gene_resource_table"   
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!|Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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Primer 2:CCTGAGCGATCATTTCCCCGACG  
Primer 2:CCTGAGCGATCATTTCCCCGACG  
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[[:Category:Kohara_Phage:20H4|20H4]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=337 Genobase]
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PMID:3038334
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est. P1 cotransduction: 10% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 10% <ref name='LIB:Nichols_linked_Tn'/>  
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[[:Category:Kohara_Phage:20H4|20H4]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=337 Genobase]
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PMID:3038334


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{|   id="V46a9d09cf2cc1"  class=" tableEdit Gene_accessions_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="V46a9d09cf2cc1"  class=" tableEdit Gene_accessions_table"   
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G7018 EcoCyc:G7018]
EcoCyc:G7018
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EcoGene]]
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB2931 EchoBASE:EB2931]
EcoGene:EG13132
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[RegulonDB]]
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[http://chase.ou.edu/macro/searchlink.php?bnum=b1858 EcoliGenExpDB:b1858]
RegulonDB:ECK120003605
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C2272 ECOL199310CYC:C2272]
[http://www.ncbi.nlm.nih.gov/gene?term=946374 GeneID:946374]
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Escherichia coli str. K-12 substr. MG1655
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EchoBASE]]
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[http://ecogene.org/geneinfo.php?eg_id=EG13132 EcoGene:EG13132]
EchoBASE:EB2931
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[ASAP]]
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[http://BioCyc.org/ECOO157/new-image?object=YEBM ECOO157CYC:YEBM]
ASAP:ABE-0006198
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0006198&formSubmitReturn=1 ASAP:ABE-0006198]
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!|Name!!URL!!Comments
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Latest revision as of 20:39, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

znuC

Mnemonic

Zn uptake

Synonyms

ECK1859, b1858, JW1847, yebM[1], yebM

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

41.83 minutes 

MG1655: 1940686..1941441
<gbrowseImage> name=NC_000913:1940686..1941441 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1921238..1921993
<gbrowseImage> name=NC_012967:1921238..1921993 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1832745..1833500
<gbrowseImage> name=NC_012759:1832745..1833500 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1944376..1945131
<gbrowseImage> name=NC_007779:1944376..1945131 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2031257..2032012
<gbrowseImage> name=NC_010473:2031257..2032012 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

znuC(del) (Keio:JW1847)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9541[3]

znuC783(del)::kan

PMID:16738554[2]

CGSC:106110

znuC783(del)::FRT

Mutagenesis rate

Decreased stress-induced mutagenesis (SIM) phenotype.

PMID:23224554[4]

Parental Strain: SMR4562

Experimental Strain: SMR12211

Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population.

CAG45114 znuC783(del)::FRTKanFRT

deletion

SigmaE activity

Decrease in sigmae activity

PMID:23224554[4]

Parental Strain: CAG45114 Experimental Strain: SMR15291

See table S11 for full experimental data.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1847

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCACAAGTCTGGTTTCCCTGGA

Primer 2:CCTGAGCGATCATTTCCCCGACG

20H4

Kohara Phage

Genobase

PMID:3038334[6]

eda-51::Tn10

Linked marker

CAG18486 = CGSC7392[3]

est. P1 cotransduction: 70% [7]

uvrC279::Tn10

Linked marker

CAG12156 = CGSC7394[3]

est. P1 cotransduction: 10% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7018

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13132

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003605

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946374

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2931

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006198

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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