znuC:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

znuC

Mnemonic

Zn uptake

Synonyms

ECK1859, b1858, JW1847, yebM[1], yebM

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

41.83 minutes 

MG1655: 1940686..1941441
<gbrowseImage> name=NC_000913:1940686..1941441 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1921238..1921993
<gbrowseImage> name=NC_012967:1921238..1921993 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1832745..1833500
<gbrowseImage> name=NC_012759:1832745..1833500 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1944376..1945131
<gbrowseImage> name=NC_007779:1944376..1945131 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2031257..2032012
<gbrowseImage> name=NC_010473:2031257..2032012 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

znuC(del) (Keio:JW1847)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9541[3]

znuC783(del)::kan

PMID:16738554[2]

CGSC:106110

znuC783(del)::FRT

Mutagenesis rate

Decreased stress-induced mutagenesis (SIM) phenotype.

PMID:23224554[4]

Parental Strain: SMR4562

Experimental Strain: SMR12211

Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population.

CAG45114 znuC783(del)::FRTKanFRT

deletion

SigmaE activity

Decrease in sigmae activity

PMID:23224554[4]

Parental Strain: CAG45114 Experimental Strain: SMR15291

See table S11 for full experimental data.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1847

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCACAAGTCTGGTTTCCCTGGA

Primer 2:CCTGAGCGATCATTTCCCCGACG

20H4

Kohara Phage

Genobase

PMID:3038334[6]

eda-51::Tn10

Linked marker

CAG18486 = CGSC7392[3]

est. P1 cotransduction: 70% [7]

uvrC279::Tn10

Linked marker

CAG12156 = CGSC7394[3]

est. P1 cotransduction: 10% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7018

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13132

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003605

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946374

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2931

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006198

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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