carB:Gene: Difference between revisions
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|{{Sub}}[[#Nomenclature |Nomenclature]] | |{{Sub}}[[#Nomenclature |Nomenclature]] | ||
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | |{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | ||
|{{Sub}}[[#Sequence Features|Sequence Features]] | |||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
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|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]] | |{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]] | ||
|} | |} | ||
</noinclude><protect> | </noinclude><protect> | ||
==Nomenclature== | ==Nomenclature== | ||
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{| id="U46a9a6f6a3850" class=" tableEdit Gene_nomenclature_table" | |||
|- | |- | ||
!{{table_heading_style}} |Standard name | !align=left {{table_heading_style}} |Standard name | ||
| | || | ||
''carB'' | ''carB'' | ||
|- | |- | ||
!{{table_heading_style}} |Mnemonic | !align=left {{table_heading_style}} |Mnemonic | ||
| | || | ||
Carbamoyl P | Carbamoyl P | ||
|- | |- | ||
!{{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
| | || | ||
''ECK0034, b0033, JW0031, arg+ura, cap, pyrA''<ref name='LIB:Riley_2006'/> | ''ECK0034, b0033, JW0031, arg+ura, cap, pyrA''<ref name='LIB:Riley_2006'/>, pyrA'' | ||
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===Notes=== | ===Notes=== | ||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="A46a9a6f7282a8" class=" tableEdit Gene_location_table" | |||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
|- | |- | ||
| | | | ||
MG1655 | MG1655 | ||
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0.66 minutes | 0.66 minutes | ||
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MG1655: 30817..34038<br /> | MG1655: 30817..34038<br /><gbrowseImage> | ||
name=NC_000913:30817..34038 | |||
source=MG1655 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10135 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10135 EcoCyc] | ||
{{GBrowseLink|MG1655|NC_000913|29817|35038|GBrowse(MG1655)}} | |||
| | | | ||
{{GBrowseFastaLink|MG1655|NC_000913|30817|34038|FASTA Dump}} | |||
*[http://biocyc.org/ECOLI/sequence?object=EG10135 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG10135 EcoCyc Sequence Dump] | ||
|- | |- | ||
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[[REL606]] | |||
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| | |||
NC_012967: 34888..38109<br /><gbrowseImage> | |||
name=NC_012967:34888..38109 | |||
source=REL606 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|REL606|NC_012967|34888|38109|GBrowse (REL606)}} | |||
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{{GBrowseFastaLink|REL606|NC_012967|34888|38109|FASTA Dump}} | |||
|- | |||
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[[BW2952]] | |||
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NC_012759: 30817..34038<br /><gbrowseImage> | |||
name=NC_012759:30817..34038 | |||
source=BW2952 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|BW2952|NC_012759|30817|34038|GBrowse (BW2952)}} | |||
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{{GBrowseFastaLink|BW2952|NC_012759|30817|34038|FASTA Dump}} | |||
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W3110 | W3110 | ||
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W3110: 30817..34038<br /> | W3110: 30817..34038<br /><gbrowseImage> | ||
name=NC_007779:30817..34038 | |||
source=W3110 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
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{{GBrowseLink|W3110|NC_007779|29817|35038|GBrowse(W3110)}} | |||
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{{GBrowseFastaLink|W3110|NC_007779|30817|34038|FASTA Dump}} | |||
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[[DH10B]] | |||
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DH10B: 30817..34038<br /><gbrowseImage> | |||
name=NC_010473:30817..34038 | |||
source=DH10B | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
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{{GBrowseLink|DH10B|NC_010473|24375|40480|GBrowse(DH10B)}} | |||
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{{GBrowseFastaLink|DH10B|NC_010473|24375|40480|FASTA Dump}} | |||
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<!--Location_notes--></protect> | <!--Location_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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{{BacktoTop}} | {{BacktoTop}} | ||
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|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Feature | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
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|[{{SERVER}} | | | ||
Coding Start (SO:0000323) | |||
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[[MG1655]] | |||
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30820 | |||
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Edman degradation | |||
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PMID:6308632<br /> | |||
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<protect><!--Sequence_notes--></protect> | <protect><!--Sequence_notes--></protect> | ||
====Notes==== | ====Notes==== | ||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P46a9a6f80ff85" class=" tableEdit Gene_allele_table dataTable" | |||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
|- | |- | ||
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''carB(del)'' (Keio:JW0031) | |||
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deletion | deletion | ||
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PMID:16738554 | |||
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] | [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] | ||
[http://cgsc2.biology.yale.edu/Strain.php?ID=106630 CGSC8349]<ref name='LIB:cgsc'/> | |||
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carB96::Tn10 | carB96::Tn10 | ||
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Synonyms: car-96::Tn10 | Synonyms: car-96::Tn10 | ||
|-class= | |- | ||
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''carB671'' | |||
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PMID:4373646 | |||
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[[cgscmutation:5037|CGSC:5037]] | |||
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''carB8(del)'' | |||
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[[cgscmutation:6595|CGSC:6595]] | |||
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''carB96::Tn10'' | |||
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[[cgscmutation:7106|CGSC:7106]] | |||
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''carB3092::Tn10kan'' | |||
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[[cgscmutation:38009|CGSC:38009]] | |||
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|- | |||
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''carB745(del)::kan'' | |||
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PMID:16738554 | |||
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[[cgscmutation:100240|CGSC:100240]] | |||
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<protect><!--Alleles_notes--></protect> | <protect><!--Alleles_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
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===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
{{BacktoTop}} | {{BacktoTop}} | ||
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|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Resource!!Resource Type!!Source!!Notes/Reference | !|Resource!!Resource Type!!Source!!Notes/Reference | ||
|- | |- | ||
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JW0031 | JW0031 | ||
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | [http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | ||
| | | | ||
PMID:16769691 | |||
Status:Clone OK | Status:Clone OK | ||
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Primer 2:CCTTTGATCTGTGCGTGCATTTC | Primer 2:CCTTTGATCTGTGCGTGCATTTC | ||
|- | |- | ||
| | |||
[[:Category:Kohara_Phage:22B12|22B12]] | |||
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Kohara Phage | |||
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=103 Genobase] | |||
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PMID:3038334 | |||
|- | |||
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[[thrA:Gene|thrA34::Tn10]] | [[thrA:Gene|thrA34::Tn10]] | ||
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CAG18442 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38985 CGSC7320]<ref name='LIB:cgsc'/> | CAG18442 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38985 CGSC7320]<ref name='LIB:cgsc'/> | ||
| | | | ||
est. P1 cotransduction: 30% <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: 30% <ref name='LIB:Nichols_linked_Tn'/> <br /> | ||
Synonyms:thr-34::Tn10 | Synonyms:thr-34::Tn10 | ||
|- | |- | ||
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leuO3051::Tn10 | leuO3051::Tn10 | ||
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CAG12095 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37901 CGSC7324]<ref name='LIB:cgsc'/> | CAG12095 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37901 CGSC7324]<ref name='LIB:cgsc'/> | ||
| | | | ||
est. P1 cotransduction: 8% <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: 8% <ref name='LIB:Nichols_linked_Tn'/> <br /> | ||
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006). | |||
Synonyms:zab-3051::Tn10 | |||
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<protect><!--Gene_resources_notes--></protect> | <protect><!--Gene_resources_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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{{BacktoTop}} | {{BacktoTop}} | ||
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|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
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[[EcoCyc]] | |||
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EcoCyc:EG10135 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[EcoGene]] | |||
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EcoGene:EG10135 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[RegulonDB]] | |||
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RegulonDB:ECK120000131 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=944775 GeneID:944775] | ||
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Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
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[[EchoBASE]] | |||
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EchoBASE:EB0133 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[ASAP]] | |||
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ASAP:ABE-0000121 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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<protect><!--Gene_accessions_notes--></protect> | <protect><!--Gene_accessions_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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===Categories=== | |||
[[Category:Gene List:MG1655]] | |||
[[Category:Gene List:W3110]] | |||
[[Category:Genes in the Keio knockout collection]] | |||
[[Category:Genes in the ASKA clone collection]] | |||
[[Category:car genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]] | |||
[[Category:Gene_List:BW2952]] | |||
Latest revision as of 20:36, 19 September 2013
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
carB |
|---|---|
| Mnemonic |
Carbamoyl P |
| Synonyms |
ECK0034, b0033, JW0031, arg+ura, cap, pyrA[1], pyrA |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
0.66 minutes |
MG1655: 30817..34038 |
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|
NC_012967: 34888..38109 |
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NC_012759: 30817..34038 |
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|
W3110 |
|
W3110: 30817..34038 |
||
|
DH10B: 30817..34038 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
30820 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
carB(del) (Keio:JW0031) |
deletion |
deletion |
|||||
|
carB96::Tn10 |
Insertion at 33,963 bp in MG1655 (NC_000913) |
adapted from Nichols et al.[5] |
Synonyms: car-96::Tn10 | ||||
|
carB671 |
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|
carB8(del) |
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|
carB96::Tn10 |
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|
carB3092::Tn10kan |
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|
carB745(del)::kan |
| ||||||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0031 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCCAAAACGTACAGATATAAA Primer 2:CCTTTGATCTGTGCGTGCATTTC | |
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 30% [5] | ||
|
leuO3051::Tn10 |
Linked marker |
est. P1 cotransduction: 8% [5] | |
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Nyunoya, H & Lusty, CJ (1983) The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase. Proc. Natl. Acad. Sci. U.S.A. 80 4629-33 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 4.3 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 5.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Mergeay, M et al. (1974) Physiology and genetics of carbamoylphosphate synthesis in Escherichia coli K12. Mol. Gen. Genet. 133 299-316 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed