carB:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
CarB |
|---|---|
| Synonyms |
carbamoyl-phosphate synthase large subunit[1], B0033[2][1], Cap[2][1], PyrA[2][1], β chain[2][1], large chain[2][1], CarB[2][1] , cap, ECK0034, JW0031, pyrA, b0033 |
| Product description |
Component of carbamoyl phosphate synthetase[2][3] Carbamoyl phosphate synthase, ammonia, large subunit; tetramer of heterodimers[4] |
| EC number (for enzymes) | |
| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000166 |
nucleotide binding |
IPI: Inferred from Physical Interaction |
F |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0000166 |
nucleotide binding |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0003824 |
catalytic activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
IMP: Inferred from Mutant Phenotype |
F |
complete | ||||
| GO:0003824 |
catalytic activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
|
Contributes to |
GO:0004088 |
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity |
IDA: Inferred from Direct Assay |
F |
replaces obsoleteGO:0004086 |
complete | ||
| GO:0003824 |
catalytic activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005515 |
protein binding |
IDA: Inferred from Direct Assay |
F |
CarA |
complete | |||
| GO:0003824 |
catalytic activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004087 |
carbamoyl-phosphate synthase (ammonia) activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0003824 |
catalytic activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0046872 |
metal ion binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0003824 |
catalytic activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0019856 |
pyrimidine base biosynthetic process |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0004086 |
carbamoyl-phosphate synthase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008652 |
cellular amino acid biosynthetic process |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0004086 |
carbamoyl-phosphate synthase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
|
Contributes to |
GO:0004088 |
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity |
IGI: Inferred from Genetic Interaction |
EcoliWiki:carA |
F |
complete | ||
| GO:0004086 |
carbamoyl-phosphate synthase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000166 |
nucleotide binding |
IMP: Inferred from Mutant Phenotype |
F |
complete | ||||
| GO:0004088 |
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000166 |
nucleotide binding |
IDA: Inferred from Direct Assay |
F |
UMP (CHEBI:16695) & IMP (CHEBI:17202) |
complete | |||
| GO:0004088 |
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000166 |
nucleotide binding |
IPI: Inferred from Physical Interaction |
F |
IMP (CHEBI:17202) |
Missing: with/from | |||
| GO:0005515 |
protein binding |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0016597 |
amino acid binding |
IDA: Inferred from Direct Assay |
F |
ornithine (CHEBI:18257) |
complete | |||
| GO:0005524 |
ATP binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016597 |
amino acid binding |
IPI: Inferred from Physical Interaction |
F |
ornithine (CHEBI:18257) |
Missing: with/from | |||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006221 |
pyrimidine nucleotide biosynthetic process |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006221 |
pyrimidine nucleotide biosynthetic process |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006526 |
arginine biosynthetic process |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006526 |
arginine biosynthetic process |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006807 |
nitrogen compound metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006807 |
nitrogen compound metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006807 |
nitrogen compound metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008152 |
metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008152 |
metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008652 |
cellular amino acid biosynthetic process |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016597 |
amino acid binding |
IPI: Inferred from Physical Interaction |
F |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0016597 |
amino acid binding |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0030145 |
manganese ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0046872 |
metal ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of carbamoyl phosphate synthetase |
could be indirect |
||
|
Protein |
ahpF |
Experiment(s):EBI-1135376 | ||
|
Protein |
zwf |
Experiment(s):EBI-1135376 | ||
|
Protein |
groL |
Experiment(s):EBI-1135376 | ||
|
Protein |
glpD |
Experiment(s):EBI-1135376 | ||
|
Protein |
fimA |
LCMS(ID Probability):99.4 | ||
|
Protein |
rpsJ |
LCMS(ID Probability):99.6 | ||
|
Protein |
cysI |
MALDI(Z-score):26.028219 | ||
|
Protein |
cysJ |
MALDI(Z-score):27.126484 | ||
|
Protein |
CarA |
|||
|
Small Molecule |
ATP |
|||
|
Small Molecule |
Mg2+ |
| ||
| edit table |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MPKRTDIKSI LILGAGPIVI GQACEFDYSG AQACKALREE GYRVILVNSN PATIMTDPEM ADATYIEPIH WEVVRKIIEK ERPDAVLPTM GGQTALNCAL ELERQGVLEE FGVTMIGATA DAIDKAEDRR RFDVAMKKIG LETARSGIAH TMEEALAVAA DVGFPCIIRP SFTMGGSGGG IAYNREEFEE ICARGLDLSP TKELLIDESL IGWKEYEMEV VRDKNDNCII VCSIENFDAM GIHTGDSITV APAQTLTDKE YQIMRNASMA VLREIGVETG GSNVQFAVNP KNGRLIVIEM NPRVSRSSAL ASKATGFPIA KVAAKLAVGY TLDELMNDIT GGRTPASFEP SIDYVVTKIP RFNFEKFAGA NDRLTTQMKS VGEVMAIGRT QQESLQKALR GLEVGATGFD PKVSLDDPEA LTKIRRELKD AGADRIWYIA DAFRAGLSVD GVFNLTNIDR WFLVQIEELV RLEEKVAEVG ITGLNADFLR QLKRKGFADA RLAKLAGVRE AEIRKLRDQY DLHPVYKRVD TCAAEFATDT AYMYSTYEEE CEANPSTDRE KIMVLGGGPN RIGQGIEFDY CCVHASLALR EDGYETIMVN CNPETVSTDY DTSDRLYFEP VTLEDVLEIV RIEKPKGVIV QYGGQTPLKL ARALEAAGVP VIGTSPDAID RAEDRERFQH AVERLKLKQP ANATVTAIEM AVEKAKEIGY PLVVRPSYVL GGRAMEIVYD EADLRRYFQT AVSVSNDAPV LLDHFLDDAV EVDVDAICDG EMVLIGGIME HIEQAGVHSG DSACSLPAYT LSQEIQDVMR QQVQKLAFEL QVRGLMNVQF AVKNNEVYLI EVNPRAARTV PFVSKATGVP LAKVAARVMA GKSLAEQGVT KEVIPPYYSV KEVVLPFNKF PGVDPLLGPE MRSTGEVMGV GRTFAEAFAK AQLGSNSTMK KHGRALLSVR EGDKERVVDL AAKLLKQGFE LDATHGTAIV LGEAGINPRL VNKVHEGRPH IQDRIKNGEY TYIINTTSGR RAIEDSRVIR RSALQYKVHY DTTLNGGFAT AMALNADATE KVISVQEMHA QIK |
| Length |
1,073 |
| Mol. Wt |
117.842 kDa |
| pI |
5.1 (calculated) |
| Extinction coefficient |
68,190 - 69,940 (calc based on 31 Y, 4 W, and 14 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 Thoden, JB et al. (2004) Long-range allosteric transitions in carbamoyl phosphate synthetase. Protein Sci. 13 2398-405 PubMed
- ↑ 6.0 6.1 6.2 6.3 Mareya, SM & Raushel, FM (1995) Mapping the structural domains of E. coli carbamoyl phosphate synthetase using limited proteolysis. Bioorg. Med. Chem. 3 525-32 PubMed
- ↑ 7.0 7.1 7.2 Kothe, M & Powers-Lee, SG (2004) Nucleotide recognition in the ATP-grasp protein carbamoyl phosphate synthetase. Protein Sci. 13 466-75 PubMed
- ↑ 8.0 8.1 8.2 Trotta, PP et al. (1974) Reversible dissociation of the monomer of glutamine-dependent carbamyl phosphate synthetase into catalytically active heavy and light subunits. J. Biol. Chem. 249 492-9 PubMed
- ↑ Rubino, SD et al. (1987) In vivo synthesis of carbamyl phosphate from NH3 by the large subunit of Escherichia coli carbamyl phosphate synthetase. J. Biol. Chem. 262 4382-6 PubMed
- ↑ 10.0 10.1 Raushel, FM et al. (1979) Paramagnetic probes for carbamoyl-phosphate synthetase: metal ion binding studies and preparation of nitroxide spin-labeled derivatives. Biochemistry 18 5562-6 PubMed
- ↑ 11.0 11.1 11.2 Guillou, F et al. (1992) Mutational analysis of carbamyl phosphate synthetase. Substitution of Glu841 leads to loss of functional coupling between the two catalytic domains of the synthetase subunit. Biochemistry 31 1656-64 PubMed
- ↑ Fresquet, V et al. (2000) Site-directed mutagenesis of the regulatory domain of Escherichia coli carbamoyl phosphate synthetase identifies crucial residues for allosteric regulation and for transduction of the regulatory signals. J. Mol. Biol. 299 979-91 PubMed
- ↑ 13.0 13.1 Delannay, S et al. (1999) Serine 948 and threonine 1042 are crucial residues for allosteric regulation of Escherichia coli carbamoylphosphate synthetase and illustrate coupling effects of activation and inhibition pathways. J. Mol. Biol. 286 1217-28 PubMed
- ↑ 14.0 14.1 14.2 14.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 15.0 15.1 15.2 15.3 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ 16.0 16.1 16.2 16.3 16.4 16.5 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0000166 ! nucleotide binding
- GO:0003824 ! catalytic activity
- GO:0005524 ! ATP binding
- GO:0004088 ! carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
- GO:0004086 ! obsolete carbamoyl-phosphate synthase activity
- GO:0005515 ! protein binding
- GO:0004087 ! carbamoyl-phosphate synthase (ammonia) activity
- GO:0046872 ! metal ion binding
- GO:0019856 ! pyrimidine nucleobase biosynthetic process
- GO:0008652 ! cellular amino acid biosynthetic process
- GO:0016597 ! amino acid binding
- GO:0006221 ! pyrimidine nucleotide biosynthetic process
- GO:0006526 ! arginine biosynthetic process
- GO:0006807 ! nitrogen compound metabolic process
- GO:0008152 ! metabolic process
- GO:0030145 ! manganese ion binding
- Proteins
