PMID:6308632
| Citation |
Nyunoya, H and Lusty, CJ (1983) The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase. Proc. Natl. Acad. Sci. U.S.A. 80:4629-33 |
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| Abstract |
Previous genetic and biochemical studies indicate that the carB gene of Escherichia coli codes for the large subunit of carbamoyl-phosphate synthetase (EC 6.3.5.5). We have determined the nucleotide sequence of a 4-kilobase-pair cloned fragment of E. coli DNA with genetic determinants for carB. The DNA sequence is a 3,219-nucleotide-long reading frame. The polypeptide encoded by this reading frame has been verified to be the large subunit of carbamoyl-phosphate synthetase. The gene product is similar to the large subunit in its molecular weight, amino acid composition and amino-terminal residue, and carboxyl-terminal sequence. The amino acid sequence derived from the nucleotide sequence shows a highly significant homology between the amino- and carboxyl-terminal halves of the protein. We propose that the carB gene was formed by an internal duplication of a smaller ancestral gene. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/genetics; Cloning, Molecular; Codon/genetics; DNA Restriction Enzymes; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Kinetics; Ligases/genetics; Operon |
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