kefA:Gene: Difference between revisions

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!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK0459, b0465, JW0454, aefA, mscK''<ref name='LIB:Riley_2006'/>
''ECK0459, b0465, JW0454, aefA, mscK''<ref name='LIB:Riley_2006'/>, mscK, pnuH''


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****************************************************************************************** -->{{Gene_location_help}}
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{|   id="Q46a9b14e207d5"  class=" tableEdit Gene_location_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Q46a9b14e207d5"  class=" tableEdit Gene_location_table"   
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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=G6255 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=G6255 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|484760|490122|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|484760|490122|GBrowse(MG1655)}}
   
   
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{{GBrowseFastaLink|ecoli|NC_000913|485760|489122|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|485760|489122|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=G6255 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=G6255 EcoCyc Sequence Dump]  
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{{GBrowseLink|BW2952|012759|388519|NC_391881|GBrowse (BW2952)}}
{{GBrowseLink|BW2952|NC_012759|388519|391881|GBrowse (BW2952)}}
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{{GBrowseFastaLink|BW2952|012759|388519|NC_391881|FASTA Dump}}  
{{GBrowseFastaLink|BW2952|NC_012759|388519|391881|FASTA Dump}}  
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W3110: 485760..489122<br /><gbrowseImage>
W3110: 485760..489122<br /><gbrowseImage>
name=AC_000091:485760..489122
name=NC_007779:485760..489122
source=MG1655_W3110
source=W3110
preset=GeneLocation
preset=GeneLocation
</gbrowseImage>
</gbrowseImage>


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{{GBrowseLink|ecoli_W3110|AC_000091|484760|490122|GBrowse(W3110)}}  
{{GBrowseLink|W3110|NC_007779|484760|490122|GBrowse(W3110)}}  
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|485760|489122|FASTA Dump}}  
{{GBrowseFastaLink|W3110|NC_007779|485760|489122|FASTA Dump}}  
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{{GBrowseLink|ecoli_DH10B|NC_010473|418367|435177|GBrowse(DH10B)}}
{{GBrowseLink|DH10B|NC_010473|418367|435177|GBrowse(DH10B)}}
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{{GBrowseFastaLink|ecoli_DH10B|NC_010473|418367|435177|FASTA Dump}}  
{{GBrowseFastaLink|DH10B|NC_010473|418367|435177|FASTA Dump}}  


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****************************************************************************************** -->{{Gene_sequence_features_help}}
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{|   id="S46a9b14f08090"  class=" tableEdit Gene_sequence_features_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="S46a9b14f08090"  class=" tableEdit Gene_sequence_features_table"   
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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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{| {{Prettytable}} id="Z46a9b14f761df"  class=" tableEdit Gene_allele_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Z46a9b14f761df"  class=" tableEdit Gene_allele_table dataTable"   
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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&Delta;kefA (Keio:JW0454)
''kefA(del)'' (Keio:JW0454)
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deletion
deletion
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PMID:16738554
PMID:16738554
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=106891 CGSC8610]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
 
[http://cgsc2.biology.yale.edu/Strain.php?ID=106891 CGSC8610]<ref name='LIB:cgsc'/>  
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''&#916;kefA750::kan''
''kefA750(del)::kan''
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{|   id="V4e04be26e5596"  class=" tableEdit Genetic_interaction_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="V4e04be26e5596"  class=" tableEdit Genetic_interaction_table dataTable"   
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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{|   id="R46a9b1505d8ba"  class=" tableEdit Gene_resource_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="R46a9b1505d8ba"  class=" tableEdit Gene_resource_table"   
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!|Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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Primer 2:CCCCCTACCGCTGGCGTCGGGTC  
Primer 2:CCCCCTACCGCTGGCGTCGGGTC  
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[[:Category:Kohara_Phage:8C4|8C4]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=151 Genobase]
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PMID:3038334
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{|   id="C46a9b151454ab"  class=" tableEdit Gene_accessions_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="C46a9b151454ab"  class=" tableEdit Gene_accessions_table"   
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G6255 EcoCyc:G6255]
[http://www.ncbi.nlm.nih.gov/gene?term=945132 GeneID:945132]
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Escherichia coli str. K-12 substr. MG1655
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[RegulonDB]]
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3991 EchoBASE:EB3991]
RegulonDB:
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[EchoBASE]]
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[http://chase.ou.edu/macro/searchlink.php?bnum=b0465 EcoliGenExpDB:b0465]
EchoBASE:
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[ASAP]]
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[http://BioCyc.org/ECOL199310/new-image?object=C0584 ECOL199310CYC:C0584]
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ASAP:
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[http://ecogene.org/geneinfo.php?eg_id=EG14240 EcoGene:EG14240]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOO157/new-image?object=AEFA ECOO157CYC:AEFA]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0001613&formSubmitReturn=1 ASAP:ABE-0001613]
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!|Name!!URL!!Comments
!|Name!!URL!!Comments

Latest revision as of 20:32, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

kefA

Mnemonic

potassium (K) efflux K+ efflux

Synonyms

ECK0459, b0465, JW0454, aefA, mscK[1], mscK, pnuH

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

10.47 minutes 

MG1655: 485760..489122
<gbrowseImage> name=NC_000913:485760..489122 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 458603..461965
<gbrowseImage> name=NC_012967:458603..461965 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 388519..391881
<gbrowseImage> name=NC_012759:388519..391881 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 485760..489122
<gbrowseImage> name=NC_007779:485760..489122 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 425091..428453
<gbrowseImage> name=NC_010473:425091..428453 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

kefA(del) (Keio:JW0454)

deletion

deletion

PMID:16738554[2]

Shigen

CGSC8610[3]

kefA750(del)::kan

PMID:16738554[2]

CGSC:101622


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0454

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCACTATGTTCCAGTATTACAA

Primer 2:CCCCCTACCGCTGGCGTCGGGTC

8C4

Kohara Phage

Genobase

PMID:3038334[5]

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[3]

est. P1 cotransduction: 37% [6]
Synonyms:zba-3054::Tn10

gsk-3055::Tn10

Linked marker

CAG12154 = CGSC7341[3]

est. P1 cotransduction: 58% [6]
Synonyms:zbb-30-55::TnlO, zba-3055::Tn10

zbaC3::Tn10

Linked marker

PMID:11985727[7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (EcoliWiki Page)

GeneID:945132

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ McLaggan, D et al. (2002) Analysis of the kefA2 mutation suggests that KefA is a cation-specific channel involved in osmotic adaptation in Escherichia coli. Mol. Microbiol. 43 521-36 PubMed

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