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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	yihS
Gene Synonym(s)	ECK3873, b3880, JW5569^[1], JW5569, manC?
Product Desc.	predicted glucosamine isomerase^[2]^[3] D-mannose isomerase; aldose-ketose isomerase inter-converting mannose, fructose and glucose; D-lyxose isomerase^[4]
Product Synonyms(s)	predicted glucosamine isomerase^[1], B3880^[2]^[1], YihS^[2]^[1] , ECK3873, JW5569, manC?, b3880
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yihS^[2]
Regulation/Activity
Quick Links
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Notes

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YihS is a weak D-mannose isomerase and a role for YihQRS in mannan or mannuronan catabolism has been suggested (Itoh, 2008). YihS may be the weak D-mannose isomerase with D-lyxose isomerase activity involved in lyxose catabolism previously mapped as manC and purified (Stevens, 1976; Stevens, 1981), however the oligomeric forms and amino acid compositions differ.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	yihS
Mnemonic	Systematic nomenclature
Synonyms	ECK3873, b3880, JW5569^[1], JW5569, manC?
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	87.69 minutes	MG1655: 4069779..4068538 <gbrowseImage> name=NC_000913:4068538..4069779 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4049903..4048662 <gbrowseImage> name=NC_012967:4048662..4049903 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3958207..3959448 <gbrowseImage> name=NC_012759:3958207..3959448 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3564925..3566166 <gbrowseImage> name=NC_007779:3564925..3566166 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4169476..4168235 <gbrowseImage> name=NC_010473:4168235..4169476 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔyihS (Keio:JW5569)	deletion	deletion	PMID:16738554	Shigen CGSC11469^[5]
ΔyihS742::kan			PMID:16738554	CGSC:105412
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5569	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAATGGTTTAACACCCTAAG Primer 2:CCTTTCGCATTAATATCCAGCAG
6H9	Kohara Phage	Genobase	PMID:3038334
9H1	Kohara Phage	Genobase	PMID:3038334
zih-35::Tn10	Linked marker	CAG18495 = CGSC7467^[5]	est. P1 cotransduction: 87% ^[6]
pflD501::Tn10	Linked marker	CAG18477 = CGSC7470^[5]	est. P1 cotransduction: 1% ^[6] Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11845	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11845	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001776	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948374	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1791	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012673	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YihS
Synonyms	predicted glucosamine isomerase^[1], B3880^[2]^[1], YihS^[2]^[1] , ECK3873, JW5569, manC?, b3880
Product description	predicted glucosamine isomerase^[2]^[3] D-mannose isomerase; aldose-ketose isomerase inter-converting mannose, fructose and glucose; D-lyxose isomerase^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004476	mannose-6-phosphate isomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010819	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006013	mannose metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010819	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRIKGMKWFN TLSHNRWLEQ ETDRIFDFGK NSVVPTGFGW LGNKGQIKEE MGTHLWITAR MLHVYSVAAA MGRPGAYSLV DHGIKAMNGA LRDKKYGGWY ACVNDEGVVD ASKQGYQHFF ALLGAASAVT TGHPEARKLL DYTIEIIEKY FWSEEEQMCL ESWDEAFSKT EEYRGGNANM HAVEAFLIVY DVTHDKKWLD RAIRVASVII HDVARNNHYR VNEHFDTQWN PLPDYNKDNP AHRFRAFGGT PGHWIEWGRL MLHIHAALEA RCEQPPAWLL EDAKGLFNAT VRDAWAPDGA DGIVYTVDWE GKPVVRERVR WPIVEAMGTA YALYTVTGDR QYETWYQTWW EYCIKYLMDY ENGSWWQELD ADNKVTTKVW DGKQDIYHLL HCLVIPRIPL APGMAPAVAA GLLDINAK
Length	418
Mol. Wt	48.018 kDa
pI	6.3 (calculated)
Extinction coefficient	145,300 - 145,925 (calc based on 20 Y, 21 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	31..387	PF07221 N-acylglucosamine 2-epimerase (GlcNAc 2-epimerase)		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yihS taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111658	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948374	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012673	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P32140	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11845	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11845	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948374	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001776	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1791	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	15	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	21	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	5^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yihS
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4069759..4069799 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3880 (EcoliWiki Page)	NCBI GEO profiles for yihS
microarray	GenExpDB:b3880 (EcoliWiki Page)	Summary of data for yihS from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to yihS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11845	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1791	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3880
EcoGene	EcoGene:EG11845	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001776	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012673	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000034937 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YIHS	From SHIGELLACYC
E. coli O157	YIHS	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:48208
Pfam (EcoliWiki Page)	PF07221 N-acylglucosamine 2-epimerase (GlcNAc 2-epimerase)
EcoCyc	EcoCyc:EG11845	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11845	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001776	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1791	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012673	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

yihS:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yihS Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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