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Expression|

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Standard Name	yhjH
Gene Synonym(s)	ECK3510, b3525, JW3493^[1], JW3493
Product Desc.	protein involved in flagellar function^[2]^[3] Cyclic-di-GMP phosphodiesterase, FlhDC-regulated; suppresses motility defect of hns mutants in multicopy^[4]
Product Synonyms(s)	EAL domain containing protein involved in flagellar function^[1], B3525^[2]^[1], YhjH^[2]^[1] , ECK3510, JW3493, b3525
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yhjH^[2]
Regulation/Activity
Quick Links
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Notes

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Overexpressed Salmonella typhimurium YhjH (STM3611) enhances motitility, counteracts cellulose biosynthesis and the rdar phenotype, and mediates cyclic-di-GMP degradation. YhjH contains a putative cyclic-di-GMP phosphodiesterase EAL domain. The increased motility of the hns supppressing E. coli yhjH clones is consistent with the model that low cyclic di-GMP favors motility over sessility. FlhDC flagellar regulon. YhjH also contains a domain homologous to the methionine-tRNA ligase dimerization appendix. yhjH mutants have reduced swimming motility, dependent on ycgR and yegE: a null ycgR mutation fully suppress the yhjH motility defect; a null yegE mutation partially suppresses yhjH; a null yedQ mutation barely suppresses yhjH; a null ydaM mutation does not suppress yhjH (Figure 3A) (Pesavento, 2008).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	yhjH
Mnemonic	Systematic nomenclature
Synonyms	ECK3510, b3525, JW3493^[1], JW3493
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	79.24 minutes, 79.24 minutes	MG1655: 3677210..3676443 <gbrowseImage> name=NC_000913:3676443..3677210 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3608679..3607912 <gbrowseImage> name=NC_012967:3607912..3608679 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3564937..3565704 <gbrowseImage> name=NC_012759:3564937..3565704 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3961228..3961995 <gbrowseImage> name=NC_007779:3961228..3961995 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3774955..3774188 <gbrowseImage> name=NC_010473:3774188..3774955 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔyhjH (Keio:JW3493)	deletion	deletion	PMID:16738554	Shigen CGSC10587^[5]
ΔyhjH780::kan			PMID:16738554	CGSC:105262
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3493	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATAAGGCAGGTTATCCAGCG Primer 2:CCTAGCGCCAGAACCGCCGTATT
25B12S	Kohara Phage	Genobase	PMID:3038334
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[5]	est. P1 cotransduction: % ^[6] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: % ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12252	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12252	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002134	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948042	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2162	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011515	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YhjH
Synonyms	EAL domain containing protein involved in flagellar function^[1], B3525^[2]^[1], YhjH^[2]^[1] , ECK3510, JW3493, b3525
Product description	protein involved in flagellar function^[2]^[3] Cyclic-di-GMP phosphodiesterase, FlhDC-regulated; suppresses motility defect of hns mutants in multicopy^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	Aspect	Notes	Status
	GO:0048870	cell motility	PMID:18765794	IMP: Inferred from Mutant Phenotype	P	Seeded from EcoCyc (v14.0)	complete
	GO:0071111	cyclic-guanylate-specific phosphodiesterase activity	PMID:18765794	IDA: Inferred from Direct Assay	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	groL		PMID:16606699	Experiment(s):EBI-1146077
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMIRQVIQRI SNPEASIESL QERRFWLQCE RAYTWQPIYQ TCGRLMAVEL LTVVTHPLNP SQRLPPDRYF TEITVSHRME VVKEQIDLLA QKADFFIEHG LLASVNIDGP TLIALRQQPK ILRQIERLPW LRFELVEHIR LPKDSTFASM CEFGPLWLDD FGTGMANFSA LSEVRYDYIK IARELFVMLR QSPEGRTLFS QLLHLMNRYC RGVIVEGVET PEEWRDVQNS PAFAAQGWFL SRPAPIETLN TAVLAL
Length	256
Mol. Wt	29.732 kDa
pI	6.4 (calculated)
Extinction coefficient	41,940 - 42,440 (calc based on 6 Y, 6 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	17..242	PF00563 EAL domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yhjH taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111605	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948042	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011515	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P37646	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12252	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12252	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948042	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002134	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2162	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	2^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yhjH
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3677190..3677230 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3525 (EcoliWiki Page)	NCBI GEO profiles for yhjH
microarray	GenExpDB:b3525 (EcoliWiki Page)	Summary of data for yhjH from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to yhjH Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12252	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2162	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3525
EcoGene	EcoGene:EG12252	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002134	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011515	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YHJH	From SHIGELLACYC
E. coli O157	YHJH	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00563 EAL domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:141868
EcoCyc	EcoCyc:EG12252	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12252	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002134	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2162	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011515	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

yhjH:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yhjH Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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