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Product(s) |

Expression|

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Standard Name	ygjK
Gene Synonym(s)	ECK3070, b3080, JW3051^[1], JW3051
Product Desc.	predicted glycosyl hydrolase^[2]^[3] alpha-Glucosidase activity in vitro, function unknown^[4]
Product Synonyms(s)	predicted glycosyl hydrolase^[1], B3080^[2]^[1], YgjK^[2]^[1] , ECK3070, JW3051, b3080
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ygjJ^[2]
Regulation/Activity
Quick Links
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Notes

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The first 22 aa are predicted to be a type I signal peptide. YgjK binds glucose, mannose, and galactose and hydrolyzes nigerose, trehalose and maltooligosaccharides, with low activities (Kurakata, 2008). Glycoside hydrolase family 63.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ygjK
Mnemonic	Systematic nomenclature
Synonyms	ECK3070, b3080, JW3051^[1], JW3051
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	69.55 minutes	MG1655: 3226910..3229261 <gbrowseImage> name=NC_000913:3226910..3229261 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3161705..3164056 <gbrowseImage> name=NC_012967:3161705..3164056 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3114058..3116409 <gbrowseImage> name=NC_012759:3114058..3116409 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3227544..3229895 <gbrowseImage> name=NC_007779:3227544..3229895 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3324655..3327006 <gbrowseImage> name=NC_010473:3324655..3327006 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔygjK (Keio:JW3051)	deletion	deletion	PMID:16738554	Shigen CGSC10329^[5]
ΔygjK770::kan			PMID:16738554	CGSC:105064
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3051	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAATAAAAACTATTTTAAC Primer 2:CCTTGCTTACGGAAAAAATCGTT
17E4	Kohara Phage	Genobase	PMID:3038334
4A1	Kohara Phage	Genobase	PMID:3038334
aer-3075::Tn10	Linked marker	CAG12152 = CGSC7438^[5]	est. P1 cotransduction: 67% ^[6] Synonyms:zgh-3075::Tn10, zgj-3075::Tn10
sfsB203::Tn10	Linked marker	CAG12072 = CGSC7440^[5]	est. P1 cotransduction: % ^[6] Synonyms:zgj-203::Tn10, zha-203::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7599	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12722	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004153	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947596	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2581	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010118	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YgjK
Synonyms	predicted glycosyl hydrolase^[1], B3080^[2]^[1], YgjK^[2]^[1] , ECK3070, JW3051, b3080
Product description	predicted glycosyl hydrolase^[2]^[3] alpha-Glucosidase activity in vitro, function unknown^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004555	alpha,alpha-trehalase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001661	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005991	trehalose metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001661	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	PMID:11967071	IEP: Inferred from Expression Pattern		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	recG	PMID:16606699	Experiment(s):EBI-1144765
Protein	fecA	PMID:16606699	Experiment(s):EBI-1144765
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKIKTILTPV TCALLISFSA HAANADNYKN VINRTGAPQY MKDYDYDDHQ RFNPFFDLGA WHGHLLPDGP NTMGGFPGVA LLTEEYINFM ASNFDRLTVW QDGKKVDFTL EAYSIPGALV QKLTAKDVQV EMTLRFATPR TSLLETKITS NKPLDLVWDG ELLEKLEAKE GKPLSDKTIA GEYPDYQRKI SATRDGLKVT FGKVRATWDL LTSGESEYQV HKSLPVQTEI NGNRFTSKAH INGSTTLYTT YSHLLTAQEV SKEQMQIRDI LARPAFYLTA SQQRWEEYLK KGLTNPDATP EQTRVAVKAI ETLNGNWRSP GGAVKFNTVT PSVTGRWFSG NQTWPWDTWK QAFAMAHFNP DIAKENIRAV FSWQIQPGDS VRPQDVGFVP DLIAWNLSPE RGGDGGNWNE RNTKPSLAAW SVMEVYNVTQ DKTWVAEMYP KLVAYHDWWL RNRDHNGNGV PEYGATRDKA HNTESGEMLF TVKKGDKEET QSGLNNYARV VEKGQYDSLE IPAQVAASWE SGRDDAAVFG FIDKEQLDKY VANGGKRSDW TVKFAENRSQ DGTLLGYSLL QESVDQASYM YSDNHYLAEM ATILGKPEEA KRYRQLAQQL ADYINTCMFD PTTQFYYDVR IEDKPLANGC AGKPIVERGK GPEGWSPLFN GAATQANADA VVKVMLDPKE FNTFVPLGTA ALTNPAFGAD IYWRGRVWVD QFWFGLKGME RYGYRDDALK LADTFFRHAK GLTADGPIQE NYNPLTGAQQ GAPNFSWSAA HLYMLYNDFF RKQ
Length	783
Mol. Wt	88.322 kDa
pI	6.1 (calculated)
Extinction coefficient	182,660 - 183,035 (calc based on 34 Y, 24 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-22	UniProt Manual:Signal Peptides	UniProt:P42592
Domain	408..770	PF01204 Trehalase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ygjK taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130975	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947596	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010118	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P42592	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7599	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12722	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947596	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004153	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2581	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	2^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ygjJ
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3226890..3226930 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3080 (EcoliWiki Page)	NCBI GEO profiles for ygjK
microarray	GenExpDB:b3080 (EcoliWiki Page)	Summary of data for ygjK from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to ygjK Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7599	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2581	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3080
EcoGene	EcoGene:EG12722	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004153	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010118	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YGJK	From SHIGELLACYC
E. coli O157	YGJK	From ECOO157CYC
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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01204 Trehalase
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

ygjK:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ygjK Expression

Notes

Links

Evolution

Homologs in Other Organisms

Notes

Families

Notes

Links

References

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