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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	yeiG
Gene Synonym(s)	ECK2147, b2154, JW2141^[1], JW2141
Product Desc.	EG12026^[2]^[3] S-formylglutathione hydrolase, formaldehyde degradation; S-lactoylglutathione hydrolase, may be involved in methylglyoxal detoxification in the cytoplasm; constitutively expressed, not induced by formaldehyde^[4]
Product Synonyms(s)	predicted esterase^[1], B2154^[2]^[1], YeiG^[2]^[1] , ECK2147, JW2141, b2154
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yeiG^[2]
Regulation/Activity
Quick Links
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Notes

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YeiG and FrmB are 50% identical paralogs; double mutant is formaldehyde sensitive. YeiG also has p-nitrophenyl C2-C6 fatty acid ester and a-naphthyl acetate carboxylesterase activities in vitro. The amino terminal half of YeiG is homologous to the C-terminal half of YieL. S9 peptidase family; YeiG or FrmB are not in MEROPS yet, but it has homologs.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	yeiG
Mnemonic	Systematic nomenclature
Synonyms	ECK2147, b2154, JW2141^[1], JW2141
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	48.32 minutes	MG1655: 2241932..2242768 <gbrowseImage> name=NC_000913:2241932..2242768 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2195148..2195984 <gbrowseImage> name=NC_012967:2195148..2195984 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2134415..2135251 <gbrowseImage> name=NC_012759:2134415..2135251 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2247244..2248080 <gbrowseImage> name=NC_007779:2247244..2248080 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2332921..2333757 <gbrowseImage> name=NC_010473:2332921..2333757 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔyeiG (Keio:JW2141)	deletion	deletion		PMID:16738554	Shigen CGSC9735^[5]
yeiGD223A		D223A	Loss of activity			seeded from UniProt:P33018
yeiGD255A		D255A	Increases affinity for substrate, but reduces catalytic efficiency			seeded from UniProt:P33018
yeiGH256A		H256A	Loss of activity			seeded from UniProt:P33018
yeiGS145A		S145A	Loss of activity			seeded from UniProt:P33018
yeiGD199A		D199A	Reduces catalytic efficiency, but has no effect on affinity for substrate			seeded from UniProt:P33018
yeiGD80A		D80A	Increases affinity for substrate, but reduces catalytic efficiency			seeded from UniProt:P33018
yeiGC26A		C26A	Reduces catalytic efficiency, but has no effect on affinity for substrate			seeded from UniProt:P33018
yeiGD218A		D218A	Reduces affinity for substrate and catalytic efficiency			seeded from UniProt:P33018
yeiGC54A		C54A	Reduces affinity for substrate and catalytic efficiency			seeded from UniProt:P33018
ΔyeiG781::kan				PMID:16738554	CGSC:104572
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2141	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGAAATGCTCGAAGAGCACCG Primer 2:CCtTTCAGTAAATACTGCGCATG
7F1	Kohara Phage	Genobase	PMID:3038334
7H12	Kohara Phage	Genobase	PMID:3038334
5H11	Kohara Phage	Genobase	PMID:3038334
zbd-3104::Tn10	Linked marker	CAG12021 = CGSC7344^[5]	est. P1 cotransduction: 2% ^[6] Synonyms:zbc-3105::Tn10, zbd-3105::Tn10
napA722::Tn10	Linked marker	CAG12098 = CGSC7400^[5]	est. P1 cotransduction: 5% ^[6] Synonyms:zeg-722, zei-722::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12026	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12026	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001943	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:949045	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1961	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007124	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YeiG
Synonyms	predicted esterase^[1], B2154^[2]^[1], YeiG^[2]^[1] , ECK2147, JW2141, b2154
Product description	EG12026^[2]^[3] S-formylglutathione hydrolase, formaldehyde degradation; S-lactoylglutathione hydrolase, may be involved in methylglyoxal detoxification in the cytoplasm; constitutively expressed, not induced by formaldehyde^[4]
EC number (for enzymes)	3.1.1.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0004091	carboxylesterase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR014186	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004091	carboxylesterase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0719	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016023	cytoplasmic membrane-bounded vesicle	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR014186	C	Seeded from EcoCyc (v14.0)	complete
	GO:0018738	S-formylglutathione hydrolase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR014186	F	Seeded from EcoCyc (v14.0)	complete
	GO:0018738	S-formylglutathione hydrolase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.1.2.12	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rplK	PMID:16606699	Experiment(s):EBI-1142034
Protein	nadE	PMID:16606699	Experiment(s):EBI-1142034
Protein	rplD	PMID:16606699	Experiment(s):EBI-1142034
Protein	tatE	PMID:16606699	Experiment(s):EBI-1142034
Protein	rplW	PMID:19402753	LCMS(ID Probability):99.6
Protein	secA	PMID:19402753	LCMS(ID Probability):99.6
Protein	yccJ	PMID:19402753	LCMS(ID Probability):99.6
Protein	glpK	PMID:19402753	LCMS(ID Probability):99.6
Protein	gpmA	PMID:19402753	MALDI(Z-score):33.496090
Protein	frmB	PMID:19402753	LCMS(ID Probability):99.6 MALDI(Z-score):34.497403
Protein	nepI	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MEMLEEHRCF EGWQQRWRHD SSTLNCPMTF SIFLPPPRDH TPPPVLYWLS GLTCNDENFT TKAGAQRVAA ELGIVLVMPD TSPRGEKVAN DDGYDLGQGA GFYLNATQPP WATHYRMYDY LRDELPALVQ SQFNVSDRCA ISGHSMGGHG ALIMALKNPG KYTSVSAFAP IVNPCSVPWG IKAFSSYLGE DKNAWLEWDS CALMYASNAQ DAIPTLIDQG DNDQFLADQL QPAVLAEAAR QKAWPMTLRI QPGYDHSYYF IASFIEDHLR FHAQYLLK
Length	278
Mol. Wt	31.259 kDa
pI	5.2 (calculated)
Extinction coefficient	63,370 - 64,120 (calc based on 13 Y, 8 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	21..271	PF00756 Putative esterase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yeiG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130092	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:949045	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007124	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P33018	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12026	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12026	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:949045	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001943	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1961	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	489	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	987	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	321	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yeiG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2241912..2241952 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2154 (EcoliWiki Page)	NCBI GEO profiles for yeiG
microarray	GenExpDB:b2154 (EcoliWiki Page)	Summary of data for yeiG from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2241600..2241941) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ07; Well:G4^[7]
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Notes

Accessions Related to yeiG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12026	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1961	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2154
EcoGene	EcoGene:EG12026	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001943	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007124	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000010587 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000000108 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Arabidopsis thaliana	AT2G41530 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000004431 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00029893 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00013018 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000006673 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000008379 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-050417-328 (score: 1.000; bootstrap: 100%) ZDB-CDNA-050417-329 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0185342 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0038771 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000027399 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000267160 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000005628 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000014128 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:95421 (score: 1.000; bootstrap: 100%) MGI:3643775 (score: 0.902) MGI:3643601 (score: 0.892)	From Inparanoid:20070104
Oryza gramene	Q5JLP6 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000009965 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000013324 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YJL068C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00003795001 (score: 1.000; bootstrap: 100%) GSTENP00007723001 (score: 0.210)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000033668 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	YEIG	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00756 Putative esterase
Superfamily (EcoliWiki Page)	SUPERFAMILY:53474
Panther (EcoliWiki Page)	PTHR10061:SF0
EcoCyc	EcoCyc:EG12026	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12026	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001943	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1961	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007124	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

yeiG:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yeiG Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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