yefM:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

yefM

Mnemonic

Systematic nomenclature

Synonyms

ECK2012, b2017, JW5835[1], JW5835

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

44.99 minutes 

MG1655: 2087737..2087486
<gbrowseImage> name=NC_000913:2087486..2087737 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1979969..1980220
<gbrowseImage> name=NC_012759:1979969..1980220 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2091850..2091599
<gbrowseImage> name=NC_007779:2091599..2091850 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2178745..2178494
<gbrowseImage> name=NC_010473:2178494..2178745 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

yefM::Tn5KAN-I-SceI (FB20573)

Insertion at nt 128 in Minus orientation

PMID:15262929[2]

E. coli Genome Project:FB20573

contains pKD46

yefM::Tn5KAN-I-SceI (FB20574)

Insertion at nt 128 in Minus orientation

PMID:15262929[2]

E. coli Genome Project:FB20574

does not contain pKD46

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5835

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCCGTACAATTAGCTACAGCGA

Primer 2:CCtTCAATGATGTCCTTTTCCGT

6D9

Kohara Phage

Genobase

PMID:3038334[4]

zef-3129::Tn10

Linked marker

CAG12099 = CGSC7397[5]

est. P1 cotransduction: 87% [6]
Synonyms:zee-3129::Tn10

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[5]

est. P1 cotransduction: % [6]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12844

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12844

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002397

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946542

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2693

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006705

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  3. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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