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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

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Standard Name	ydjI
Gene Synonym(s)	ECK1771, b1773, JW1762^[1], JW1762
Product Desc.	predicted aldolase^[2]^[3] Putative sugar 1,6-bisphosphate aldolase, function unknown^[4]
Product Synonyms(s)	predicted aldolase^[1], B1773^[2]^[1], YdjI^[2]^[1] , ECK1771, JW1762, b1773
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ydjG^[2]
Regulation/Activity
Quick Links
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Notes

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kbaY, gatY, fbaA and ydjI are paralogs.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ydjI
Mnemonic	Systematic nomenclature
Synonyms	ECK1771, b1773, JW1762^[1], JW1762
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	39.98 minutes	MG1655: 1855793..1854957 <gbrowseImage> name=NC_000913:1854957..1855793 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1834998..1834162 <gbrowseImage> name=NC_012967:1834162..1834998 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1747016..1747852 <gbrowseImage> name=NC_012759:1747016..1747852 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1859483..1858647 <gbrowseImage> name=NC_007779:1858647..1859483 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
Strain DH10B		I268F	Nonsynonomous mutation	PMID:18245285	ASAP	This is a SNP in E. coli K-12 Strain DH10B
ΔydjI (Keio:JW1762)	deletion	deletion		PMID:16738554	Shigen CGSC9481^[5]
ΔydjI771::kan				PMID:16738554	CGSC:104354
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1762	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCTCGCAGATATCAGATATTG Primer 2:CCTTCCGCTTTGCCATCAGAACC
12H7	Kohara Phage	Genobase	PMID:3038334
3E12	Kohara Phage	Genobase	PMID:3038334
zdj-276::Tn10	Linked marker	CAG18464 = CGSC7387^[5]	est. P1 cotransduction: 44% ^[6] Synonyms:zdi-276::Tn10
zea-225::Tn10	Linked marker	CAG18465 = CGSC7388^[5]	est. P1 cotransduction: 59% ^[6] Synonyms:zdj-225::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6960	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13485	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003548	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946291	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3258	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005906	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YdjI
Synonyms	predicted aldolase^[1], B1773^[2]^[1], YdjI^[2]^[1] , ECK1771, JW1762, b1773
Product description	predicted aldolase^[2]^[3] Putative sugar 1,6-bisphosphate aldolase, function unknown^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013785	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004332	fructose-bisphosphate aldolase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000771	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006096	glycolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000771	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013785	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000771	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	prsA	PMID:15690043	Experiment(s):EBI-884468
Protein	ydeP	PMID:15690043	Experiment(s):EBI-884468
Protein	dsbG	PMID:15690043	Experiment(s):EBI-884468
Protein	rplL	PMID:15690043	Experiment(s):EBI-884468
Protein	cbrA	PMID:15690043	Experiment(s):EBI-884468
Protein	zwf	PMID:16606699	Experiment(s):EBI-1140766
Protein	sucB	PMID:16606699	Experiment(s):EBI-1140766
Protein	prs	PMID:19402753	LCMS(ID Probability):99.6
Protein	dsbG	PMID:19402753	LCMS(ID Probability):99.6
Protein	ydeP	PMID:19402753	LCMS(ID Probability):99.6
Protein	ydjF	PMID:19402753	MALDI(Z-score):17.053160
Protein	cbrA	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplL	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MLADIRYWEN DATNKHYAIA HFNVWNAEML MGVIDAAEEA KSPVIISFGT GFVGNTSFED FSHMMVSMAQ KATVPVITHW DHGRSMEIIH NAWTHGMNSL MRDASAFDFE ENIRLTKEAV DFFHPLGIPV EAELGHVGNE TVYEEALAGY HYTDPDQAAE FVERTGCDSL AVAIGNQHGV YTSEPQLNFE VVKRVRDAVS VPLVLHGASG ISDADIKTAI SLGIAKINIH TELCQAAMVA VKENQDQPFL HLEREVRKAV KERALEKIKL FGSDGKAE
Length	278
Mol. Wt	30.809 kDa
pI	5.1 (calculated)
Extinction coefficient	30,940 - 31,190 (calc based on 6 Y, 4 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..277	PF01116 Fructose-bisphosphate aldolase class-II		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ydjI taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129727	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946291	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005906	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P77704	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6960	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13485	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946291	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003548	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3258	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	19	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	17	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	17^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ydjG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1855773..1855813 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1773 (EcoliWiki Page)	NCBI GEO profiles for ydjI
microarray	GenExpDB:b1773 (EcoliWiki Page)	Summary of data for ydjI from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to ydjI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6960	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3258	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1773
EcoGene	EcoGene:EG13485	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003548	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005906	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	S1565	From SHIGELLACYC
E. coli O157	Z2811	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01116 Fructose-bisphosphate aldolase class-II
Superfamily (EcoliWiki Page)	SUPERFAMILY:51569
EcoCyc	EcoCyc:G6960	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13485	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003548	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3258	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005906	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

ydjI:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ydjI Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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