ydiB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ydiB

Mnemonic

Systematic nomenclature

Synonyms

ECK1690, b1692, JW1682, ydiB'[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

38.19 minutes 

MG1655: 1771813..1772679
<gbrowseImage> name=NC_000913:1771813..1772679 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1751055..1751921
<gbrowseImage> name=NC_012967:1751055..1751921 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1663872..1664738
<gbrowseImage> name=NC_012759:1663872..1664738 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1775503..1776369
<gbrowseImage> name=NC_007779:1775503..1776369 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1862384..1863250
<gbrowseImage> name=NC_010473:1862384..1863250 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔydiB (Keio:JW1682)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9425[4]

ydiBT106A

T106A

Increases KM for quinate 2000-fold. Increases KM for shikimate 70-fold

seeded from UniProt:P0A6D5

ydiBN92A

N92A

Alters protein structure. Loss of activity

seeded from UniProt:P0A6D5

ydiBD107A

D107A

Loss of activity towards quinate. Increases KM for shikimate 20000-fold

seeded from UniProt:P0A6D5

ydiBK71A

K71A

Increases KM for quinate 3200-fold. Increases KM for shikimate 170-fold

seeded from UniProt:P0A6D5

ydiBS67A

S67A

Reduces activity towards quinate about 6-fold

seeded from UniProt:P0A6D5

ΔydiB::kan

deletion

Biolog:respiration

unable to respire N-Acetyl-D-glucosamine

PMID:16095938[5]

ΔydiB::kan

deletion

Biolog:respiration

unable to respire a-D-Glucose-1-phosphate

PMID:16095938[5]

ΔydiB::kan

deletion

Biolog:respiration

unable to respire a-D-Glucose-6-phosphate

PMID:16095938[5]

ΔydiB::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[5]

ΔydiB766::kan

PMID:16738554[3]

CGSC:104314


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1682

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGATGTTACCGCAAAATACGA

Primer 2:CCGGCACCGAACCCCATGACCTG

15G10

Kohara Phage

Genobase

PMID:3038334[7]

8G8

Kohara Phage

Genobase

PMID:3038334[7]

zdd-235::Tn10

Linked marker

CAG18461 = CGSC7378[4]

est. P1 cotransduction: % [8]
Synonyms:zdc-235::Tn10

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[4]

est. P1 cotransduction: 84% [8]
Synonyms:zdh-925::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11234

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11234

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001212

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946200

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1216

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005651

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 5.2 5.3 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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