ydhX:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

ydhX

Gene Synonym(s)

ECK1667, b1671, JW5271[1], JW5271

Product Desc.

predicted 4Fe-4S ferredoxin-type protein[2][3]

Ferredoxin-like protein, function unknown; FNR, NarL and NarP regulons[4]

Product Synonyms(s)

predicted 4Fe-4S ferridoxin-type protein[1], B1671[2][1], YdhX[2][1] , ECK1667, JW5271, b1671

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ydhT[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Verified Tat substrate: YdhX has a Tat/Sec (Class II) predicted 44 aa signal peptide (Tullman-Ercek, 2007).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ydhX

Mnemonic

Systematic nomenclature

Synonyms

ECK1667, b1671, JW5271[1], JW5271

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

37.68 minutes 

MG1655: 1749037..1748369
<gbrowseImage> name=NC_000913:1748369..1749037 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1728280..1727612
<gbrowseImage> name=NC_012967:1727612..1728280 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1640428..1641147
<gbrowseImage> name=NC_012759:1640428..1641147 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1752727..1752059
<gbrowseImage> name=NC_007779:1752059..1752727 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1839608..1838940
<gbrowseImage> name=NC_010473:1838940..1839608 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔydhX (Keio:JW5271)

deletion

deletion

PMID:16738554

Shigen
CGSC11283[5]

ΔydhX746::kan

PMID:16738554

CGSC:104310


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5271

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCCTTCACTCGACGCAAATT

Primer 2:CCTACATTTTCCTTTTTAATCAA

13H4

Kohara Phage

Genobase

PMID:3038334

zdd-235::Tn10

Linked marker

CAG18461 = CGSC7378[5]

est. P1 cotransduction: % [6]
Synonyms:zdc-235::Tn10

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[5]

est. P1 cotransduction: 33% [6]
Synonyms:zdh-925::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6899

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13958

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003488

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947308

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3716

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005583

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YdhX

Synonyms

predicted 4Fe-4S ferridoxin-type protein[1], B1671[2][1], YdhX[2][1] , ECK1667, JW5271, b1671

Product description

predicted 4Fe-4S ferredoxin-type protein[2][3]

Ferredoxin-like protein, function unknown; FNR, NarL and NarP regulons[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

GO:0009055

electron carrier activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017900

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0051536

iron-sulfur cluster binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017900

F

Seeded from EcoCyc (v14.0)

complete

GO:0051536

iron-sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0411

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0004

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

ycbZ

PMID:19402753

MALDI(Z-score):26.707864

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSWIGWTVAA TALGDNQMSF TRRKFVLGMG TVIFFTGSAS SLLANTRQEK EVRYAMIHDE
SRCNGCNICA RACRKTNHVP AQGSRLSIAH IPVTDNDNET QYHFFRQSCQ HCEDAPCIDV
CPTGASWRDE QGIVRVEKSQ CIGCSYCIGA CPYQVRYLNP VTKVADKCDF CAESRLAKGF
PPICVSACPE HALIFGREDS PEIQAWLQQN KYYQYQLPGA GKPHLYRRFG QHLIKKENV
Length

239

Mol. Wt

26.894 kDa

pI

8.2 (calculated)

Extinction coefficient

35,410 - 37,410 (calc based on 9 Y, 4 W, and 16 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ydhX taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:226524717

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947308

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005583

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P77375

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6899

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13958

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947308

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003488

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3716

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

2a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

6a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

1a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ydhT

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1749017..1749057 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1671 (EcoliWiki Page)

NCBI GEO profiles for ydhX

microarray

GenExpDB:b1671 (EcoliWiki Page)

Summary of data for ydhX from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to ydhX Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6899

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3716

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1671

EcoGene

EcoGene:EG13958

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003488

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005583

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Xenopus tropicalis

  • ENSXETP00000047835 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

S1831

From SHIGELLACYC

E. coli O157

Z2698

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:54862

EcoCyc

EcoCyc:G6899

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13958

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003488

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3716

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005583

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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