ydeI:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

ydeI

Gene Synonym(s)

ECK1529, b1536, JW1529[1], JW1529

Product Desc.

conserved protein[2][3]

Predicted secreted protein, function unknown[4]

Product Synonyms(s)

conserved protein[1], B1536[2][1], YdeI[2][1] , ECK1529, JW1529, b1536

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ydeI[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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First 19 aa are predicted to be a type I signal peptide. ydeI and ygiW are paralogs. Secreted protein, function unknown[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ydeI

Mnemonic

Systematic nomenclature

Synonyms

ECK1529, b1536, JW1529[1], JW1529

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

34.96 minutes 

MG1655: 1622521..1622129
<gbrowseImage> name=NC_000913:1622129..1622521 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1592263..1591871
<gbrowseImage> name=NC_012967:1591871..1592263 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1514188..1514580
<gbrowseImage> name=NC_012759:1514188..1514580 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1626211..1625819
<gbrowseImage> name=NC_007779:1625819..1626211 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1712146..1711754
<gbrowseImage> name=NC_010473:1711754..1712146 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔydeI (Keio:JW1529)

deletion

deletion

PMID:16738554

Shigen
CGSC9316[5]

ΔydeI757::kan

PMID:16738554

CGSC:104220


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1529

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAATTTCAGGCCATTGTATT

Primer 2:CCTTTCTGCAAATGAGTGACCCG

2H2

Kohara Phage

Genobase

PMID:3038334

2B2

Kohara Phage

Genobase

PMID:3038334

zdd-235::Tn10

Linked marker

CAG18461 = CGSC7378[5]

est. P1 cotransduction: % [6]
Synonyms:zdc-235::Tn10

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[5]

est. P1 cotransduction: % [6]
Synonyms:zdh-925::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11644

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11644

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001588

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946068

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1597

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005127

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YdeI

Synonyms

conserved protein[1], B1536[2][1], YdeI[2][1] , ECK1529, JW1529, b1536

Product description

conserved protein[2][3]

Predicted secreted protein, function unknown[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0044011

single-species biofilm formation on inanimate substrate

PMID:19919618

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0070301

cellular response to hydrogen peroxide

PMID:19919618

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

uspE

PMID:19402753

LCMS(ID Probability):98.2

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKFQAIVLAS FLVMPYALAD DQGGLKQDAA PPPPHAIEDG YRGTDDAKKM TVDFAKTMHD
GASVSLRGNL ISHKGEDRYV FRDKSGEINV VIPAAVFDGR EVQPDQMINI SGSLDKKSAP
AVVRVTHLQK
Length

130

Mol. Wt

14.12 kDa

pI

6.9 (calculated)

Extinction coefficient

4,470 (calc based on 3 Y, 0 W, and 0 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-19

UniProt Manual:Signal Peptides

UniProt:P31130

Domain

1..130

PF04076 Bacterial OB fold (BOF) protein

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ydeI taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129495

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946068

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005127

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P31130

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11644

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11644

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946068

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001588

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1597

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

0.653+/-0.342

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.100765306

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

34a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

213

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

27a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ydeI

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1622501..1622541 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1536 (EcoliWiki Page)

NCBI GEO profiles for ydeI

microarray

GenExpDB:b1536 (EcoliWiki Page)

Summary of data for ydeI from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (1622445..1622848) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ13; Well:A11[7]

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Notes

Accessions Related to ydeI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11644

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1597

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1536

EcoGene

EcoGene:EG11644

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001588

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005127

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

YDEI

From SHIGELLACYC

E. coli O157

YDEI

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF04076 Bacterial OB fold (BOF) protein

Superfamily (EcoliWiki Page)

SUPERFAMILY:101756

EcoCyc

EcoCyc:EG11644

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11644

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001588

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1597

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005127

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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