ybaY:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

ybaY

Gene Synonym(s)

ECK0447, b0453, JW0443[1], JW0443

Product Desc.

predicted outer membrane lipoprotein[2][3]

Novel verified lipoprotein, function unknown[4]

Product Synonyms(s)

predicted outer membrane lipoprotein[1], B0453[2][1], YbaY[2][1] , ECK0447, JW0443, b0453

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ybaY[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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First 18 aa are a type II signal peptide. Repressed by OxyS overexpression (Tjaden, 2006).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ybaY

Mnemonic

Systematic nomenclature

Synonyms

ECK0447, b0453, JW0443[1], JW0443

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

10.23 minutes 

MG1655: 474603..475175
<gbrowseImage> name=NC_000913:474603..475175 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 447446..448018
<gbrowseImage> name=NC_012967:447446..448018 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 377362..377934
<gbrowseImage> name=NC_012759:377362..377934 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 474603..475175
<gbrowseImage> name=NC_007779:474603..475175 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 413934..414506
<gbrowseImage> name=NC_010473:413934..414506 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔybaY (Keio:JW0443)

deletion

deletion

PMID:16738554

Shigen
CGSC8604[5]

ΔybaY739::kan

PMID:16738554

CGSC:103652


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0443

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAACTCGTGCACATGGCCAG

Primer 2:CCATATTGCGTAGGAGCTGGAAC

7E2

Kohara Phage

Genobase

PMID:3038334

19B7

Kohara Phage

Genobase

PMID:3038334

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[5]

est. P1 cotransduction: 58% [6]
Synonyms:zba-3054::Tn10

gsk-3055::Tn10

Linked marker

CAG12154 = CGSC7341[5]

est. P1 cotransduction: 36% [6]
Synonyms:zbb-30-55::TnlO, zba-3055::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6250

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13253

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002860

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945070

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3042

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001574

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YbaY

Synonyms

predicted outer membrane lipoprotein[1], B0453[2][1], YbaY[2][1] , ECK0447, JW0443, b0453

Product description

predicted outer membrane lipoprotein[2][3]

Novel verified lipoprotein, function unknown[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0031225

anchored to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9901

C

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rplS

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsN

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplP

PMID:19402753

LCMS(ID Probability):99.6

Protein

gadB

PMID:19402753

LCMS(ID Probability):99.6

Protein

atpD

PMID:19402753

LCMS(ID Probability):99.5

Protein

ftsZ

PMID:19402753

LCMS(ID Probability):99.6

Protein

fimA

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplM

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplJ

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplA

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsC

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsD

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsT

PMID:19402753

LCMS(ID Probability):99.6

Protein

yfiD

PMID:19402753

LCMS(ID Probability):99.6

Protein

hdeB

PMID:19402753

LCMS(ID Probability):99.3

Protein

ompC

PMID:19402753

LCMS(ID Probability):99.6

Protein

dps

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsJ

PMID:19402753

LCMS(ID Probability):99.6

Protein

ygaU

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplD

PMID:19402753

LCMS(ID Probability):99.6

Protein

ompX

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Outer membrane

PMID:15174130

EchoLocation:ybaY


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKLVHMASGL AVAIALAACA DKSADIQTPA PAANTSISAT QQPAIQQPNV SGTVWIRQKV
ALPPDAVLTV TLSDASLADA PSKVLAQKAV RTEGKQSPFS FVLSFNPADV QPNARILLSA
AITVNDKLVF ITDTVQPVIN QGGTKADLTL VPVQQTAVPV QASGGATTTV PSTSPTQVNP
SSAVPAPTQY
Length

190

Mol. Wt

19.43 kDa

pI

8.2 (calculated)

Extinction coefficient

6,990 - 7,115 (calc based on 1 Y, 1 W, and 1 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-18

UniProt Manual:Signal Peptides

UniProt:P77717


<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ybaY taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:49176025

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945070

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001574

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P77717

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6250

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13253

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945070

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002860

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3042

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

559

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2510

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

285

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ybaY

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:474583..474623 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0453 (EcoliWiki Page)

NCBI GEO profiles for ybaY

microarray

GenExpDB:b0453 (EcoliWiki Page)

Summary of data for ybaY from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (474316..474669) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ08; Well:G7[7]

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Notes

Accessions Related to ybaY Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6250

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3042

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0453

EcoGene

EcoGene:EG13253

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002860

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001574

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

YBAY

From SHIGELLACYC

E. coli O157

YBAY

From ECOO157CYC

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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database Accession Notes

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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