yajL:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

yajL

Mnemonic

Systematic nomenclature

Synonyms

ECK0418, b0424, JW5057, thiJ[1](incorrect), thiJ

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Notes

"YajL was mistakenly identified as ThiJ and was implicated in thiamine biosynthesis. However, later experiments showed that the original complementation phenotype was due to the neighboring gene, thiI [2]. The biochemical function of YajL is therefore unclear." (from EcoCyc)

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

9.53 minutes 

MG1655: 442865..442275
<gbrowseImage> name=NC_000913:442275..442865 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 411473..412063
<gbrowseImage> name=NC_012967:411473..412063 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 345034..345624
<gbrowseImage> name=NC_012759:345034..345624 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 442865..442275
<gbrowseImage> name=NC_007779:442275..442865 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 382196..381606
<gbrowseImage> name=NC_010473:381606..382196 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

yajL(del) (Keio:JW5057)

deletion

deletion

PMID:16738554[3]

Shigen


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5057

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAGCGCATCGGCACTGGTTTG

Primer 2:CCtTCGTAATAATTATAAATCCC

19F6

Kohara Phage

Genobase

PMID:3038334[5]

2H5

Kohara Phage

Genobase

PMID:3038334[5]

tsx-247::Tn10

Linked marker

CAG12148 = CGSC7337[6]

est. P1 cotransduction: 69% [7]

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[6]

est. P1 cotransduction: 54% [7]
Synonyms:zba-3054::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G198

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13272

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002673

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945066

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3057

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001472

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Mueller, EG et al. (1998) Identification of a gene involved in the generation of 4-thiouridine in tRNA. Nucleic Acids Res. 26 2606-10 PubMed
  3. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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