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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	wzc
Gene Synonym(s)	ECK2054, b2060, JW2045^[1], JW2045
Product Desc.	tyrosine kinase involved in colanic acid biosynthesis^[2]^[3]; Component of capsular polysaccharide export apparatus^[3] Autophosphorylating protein tyrosine kinase Wzc, a regulator of capsule production; mutants do not make capsules; cognate with Wzb phosphatase; phosphorylates Ugd^[4]
Product Synonyms(s)	protein-tyrosine kinase^[1], B2060^[2]^[1], Wzc^[2]^[1] , ECK2054, JW2045, b2060
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): wza-wzb-wzc-wcaAB^[2], OP00290, cps, cpsB, cpsGB
Regulation/Activity
Quick Links
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Notes

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Wzc, but not Wzc-P, activates colanic acid synthesis, needed for capsule production. Wzc-P is dephosphorylated in vivo and in vitro by the Wzb phosphatase. Wzc-P is also dephosphorylated in vitro by the cryptic Etp phosphatase. Wzc has ATPAse activity (Soulat, 2007).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	wzc
Mnemonic	Systematic nomenclature
Synonyms	ECK2054, b2060, JW2045^[1], JW2045
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	45.94 minutes, 45.94 minutes	MG1655: 2133676..2131514 <gbrowseImage> name=NC_000913:2131514..2133676 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2068858..2066696 <gbrowseImage> name=NC_012967:2066696..2068858 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2023997..2026159 <gbrowseImage> name=NC_012759:2023997..2026159 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2137789..2135627 <gbrowseImage> name=NC_007779:2135627..2137789 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2224684..2222522 <gbrowseImage> name=NC_010473:2222522..2224684 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
Δwzc (Keio:JW2045)	deletion	deletion		PMID:16738554	Shigen CGSC9680^[5]
wzcY491F		Y491F	No loss of autophosphorylation			seeded from UniProt:P76387
wzcK540M		K540M	Loss of autophosphorylation			seeded from UniProt:P76387
wzcY636F		Y636F	No loss of autophosphorylation			seeded from UniProt:P76387
wzcY467F		Y467F	No loss of autophosphorylation			seeded from UniProt:P76387
wzcY569F		Y569F	Loss of autophosphorylation			seeded from UniProt:P76387
wzcY668F		Y668F	No loss of autophosphorylation			seeded from UniProt:P76387
Δwzc-758::kan				PMID:16738554	CGSC:103394
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2045	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACAGAAAAAGTAAAACAACA Primer 2:CCTTTCGCATCCGACTTATATTC
10A9	Kohara Phage	Genobase	PMID:3038334
7A3	Kohara Phage	Genobase	PMID:3038334
2E1	Kohara Phage	Genobase	PMID:3038334
zef-3129::Tn10	Linked marker	CAG12099 = CGSC7397^[5]	est. P1 cotransduction: 11% ^[6] Synonyms:zee-3129::Tn10
zbd-3104::Tn10	Linked marker	CAG12021 = CGSC7344^[5]	est. P1 cotransduction: 14% ^[6] Synonyms:zbc-3105::Tn10, zbd-3105::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7105	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13568	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003691	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946567	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3338	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006819	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Wzc
Synonyms	protein-tyrosine kinase^[1], B2060^[2]^[1], Wzc^[2]^[1] , ECK2054, JW2045, b2060
Product description	tyrosine kinase involved in colanic acid biosynthesis^[2]^[3]; Component of capsular polysaccharide export apparatus^[3] Autophosphorylating protein tyrosine kinase Wzc, a regulator of capsule production; mutants do not make capsules; cognate with Wzb phosphatase; phosphorylates Ugd^[4]
EC number (for enzymes)	2.7.1.112^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000271	polysaccharide biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0270	P	Seeded from EcoCyc (v14.0)	complete
	GO:0004713	protein tyrosine kinase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0829	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009103	lipopolysaccharide biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003856	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009236	cobalamin biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002586	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003856	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016301	kinase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0418	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030234	enzyme regulator activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005702	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042242	cobyrinic acid a,c-diamide synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002586	F	Seeded from EcoCyc (v14.0)	complete
	GO:0045227	capsule polysaccharide biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005702	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of capsular polysaccharide export apparatus	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 2 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MGAGIERRAG IRMTEKVKQH AAPVTGSDEI DIGRLVGTVI EARWWVIGIT TVFALCAVVY TFFATPIYSA DALVQIEQNS GNSLVQDIGS ALANKPPASD AEIQLIRSRL VLGKTVDDLD LDIAVSKNTF PIFGAGWDRL MGRQNETVKV TTFNRPKEMA DQVFTLNVLD NKNYTLSSDG GFSARGQAGQ MLKKEGVTLM VEAIHASPGS EFTVTKYSTL GMINQLQNSL TVTENGKDAG VLSLTYTGED REQIRDILNS IARNYQEQNI ERKSAEASKS LAFLAQQLPE VRSRLDVAEN KLNAFRQDKD SVDLPLEAKA VLDSMVNIDA QLNELTFKEA EISKLYTKVH PAYRTLLEKR QALEDEKAKL NGRVTAMPKT QQEIVRLTRD VESGQQVYMQ LLNKEQELKI TEASTVGDVR IVDPAITQPG VLKPKKGLII LGAIILGLML SIVGVLLRSL FNRGIESPQV LEEHGISVYA SIPLSEWQKA RDSVKTIKGI KRYKQSQLLA VGNPTDLAIE AIRSLRTSLH FAMMQAQNNV LMMTGVSPSI GKTFVCANLA AVISQTNKRV LLIDCDMRKG YTHELLGTNN VNGLSEILIG QGDITTAAKP TSIAKFDLIP RGQVPPNPSE LLMSERFAEL VNWASKNYDL VLIDTPPILA VTDAAIVGRH VGTTLMVARY AVNTLKEVET SLSRFEQNGI PVKGVILNSI FRRASAYQDY GYYEYEYKSD AK
Length	732
Mol. Wt	80.613 kDa
pI	7.6 (calculated)
Extinction coefficient	57,300 - 57,675 (calc based on 20 Y, 5 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	16..252	PF02706 Chain length determinant protein	PMID:19920124
Domain	529..716	PF01656 CobQ/CobB/MinD/ParA nucleotide binding domain	PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=wzc taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111377	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946567	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006819	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P76387	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7105	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13568	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946567	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003691	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3338	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
wza-wzb-wzc-wcaAB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2133656..2133696 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2060 (EcoliWiki Page)	NCBI GEO profiles for wzc
microarray	GenExpDB:b2060 (EcoliWiki Page)	Summary of data for wzc from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to wzc Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7105	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3338	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2060
EcoGene	EcoGene:EG13568	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003691	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006819	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	S2248	From SHIGELLACYC
E. coli O157	Z3224	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02706 Chain length determinant protein
Pfam (EcoliWiki Page)	PF01656 CobQ/CobB/MinD/ParA nucleotide binding domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:52540
Panther (EcoliWiki Page)	PTHR13696:SF16
EcoCyc	EcoCyc:G7105	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13568	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003691	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3338	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006819	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

wzc:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to wzc Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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