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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

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Standard Name	wcaK
Gene Synonym(s)	ECK2039, b2045, JW2030^[1], JW2030
Product Desc.	predicted colanic acid biosynthesis pyruvyl transferase^[2]^[3] Colanic acid biosynthesis protein^[4]
Product Synonyms(s)	predicted pyruvyl transferase^[1], B2045^[2]^[1], WcaK^[2]^[1] , ECK2039, JW2030, b2045
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): wcaM^[2]
Regulation/Activity
Quick Links
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Notes

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Overexpression causes a decrease in the amount of biofilm formed.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	wcaK
Mnemonic	Systematic nomenclature
Synonyms	ECK2039, b2045, JW2030^[1], JW2030
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	45.59 minutes	MG1655: 2116428..2115148 <gbrowseImage> name=NC_000913:2115148..2116428 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2051635..2050355 <gbrowseImage> name=NC_012967:2050355..2051635 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2007631..2008911 <gbrowseImage> name=NC_012759:2007631..2008911 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2120541..2119261 <gbrowseImage> name=NC_007779:2119261..2120541 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2207436..2206156 <gbrowseImage> name=NC_010473:2206156..2207436 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔwcaK (Keio:JW2030)	deletion	deletion	PMID:16738554	Shigen CGSC9667^[5]
ΔwcaK743::kan			PMID:16738554	CGSC:103382
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2030	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAATTACTTATTCTGGGCAA Primer 2:CCTTTCACCTCCCCGATGCGCTC
1G1	Kohara Phage	Genobase	PMID:3038334
10A9	Kohara Phage	Genobase	PMID:3038334
zef-3129::Tn10	Linked marker	CAG12099 = CGSC7397^[5]	est. P1 cotransduction: 28% ^[6] Synonyms:zee-3129::Tn10
zbd-3104::Tn10	Linked marker	CAG12021 = CGSC7344^[5]	est. P1 cotransduction: 4% ^[6] Synonyms:zbc-3105::Tn10, zbd-3105::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7096	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13577	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003682	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946569	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3347	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006780	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	WcaK
Synonyms	predicted pyruvyl transferase^[1], B2045^[2]^[1], WcaK^[2]^[1] , ECK2039, JW2030, b2045
Product description	predicted colanic acid biosynthesis pyruvyl transferase^[2]^[3] Colanic acid biosynthesis protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0009103	lipopolysaccharide biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0448	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007345	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rplA	PMID:16606699	Experiment(s):EBI-1141740
Protein	narZ	PMID:16606699	Experiment(s):EBI-1141740
Protein	rpoB	PMID:16606699	Experiment(s):EBI-1141740
Protein	ymfG	PMID:16606699	Experiment(s):EBI-1141740
Protein	slyD	PMID:16606699	Experiment(s):EBI-1141740
Protein	rpsE	PMID:16606699	Experiment(s):EBI-1141740
Protein	rpsI	PMID:16606699	Experiment(s):EBI-1141740
Protein	rplB	PMID:16606699	Experiment(s):EBI-1141740
Protein	rpsD	PMID:16606699	Experiment(s):EBI-1141740
Protein	nrdI	PMID:16606699	Experiment(s):EBI-1141740
Protein	rusA	PMID:16606699	Experiment(s):EBI-1141740
Protein	potE	PMID:16606699	Experiment(s):EBI-1141740
Protein	ftsL	PMID:16606699	Experiment(s):EBI-1141740
Protein	rpoC	PMID:16606699	Experiment(s):EBI-1141740
Protein	lacZ	PMID:16606699	Experiment(s):EBI-1141740
Protein	rpsC	PMID:16606699	Experiment(s):EBI-1141740
Protein	rpsG	PMID:16606699	Experiment(s):EBI-1141740
Protein	rpsM	PMID:16606699	Experiment(s):EBI-1141740
Protein	pspE	PMID:16606699	Experiment(s):EBI-1141740
Protein	yghW	PMID:16606699	Experiment(s):EBI-1141740
Protein	rplQ	PMID:16606699	Experiment(s):EBI-1141740
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKLLILGNHT CGNRGDSAIL RGLLDAINIL NPHAEVDVMS RYPVSSSWLL NRPVMGDPLF LQMKQHNSAA GVVGRVKKVL RRRYQHQVLL SRVTDTGKLR NIAIAQGFTD FVRLLSGYDA IIQVGGSFFV DLYGVPQFEH ALCTFMAKKP LFMIGHSVGP FQDEQFNQLA NYVFGHCDAL ILRESVSFDL MKRSNITTAK VEHGVDTAWL VDHHTEDFTA SYAVQHWLDV AAQQKTVAIT LRELAPFDKR LGTTQQAYEK AFAGVVNRIL DEGYQVIALS TCTGIDSYNK DDRMVALNLR QHISDPARYH VVMDELNDLE MGKILGACEL TVGTRLHSAI ISMNFATPAI AINYEHKSAG IMQQLGLPEM AIDIRHLLDG SLQAMVADTL GQLPALNARL SEAVSRERQT GMQMVQSVLE RIGEVK
Length	426
Mol. Wt	47.343 kDa
pI	7.1 (calculated)
Extinction coefficient	32,890 - 33,515 (calc based on 11 Y, 3 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	13..356	PF04230 Polysaccharide pyruvyl transferase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=wcaK taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129985	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946569	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006780	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P71242	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7096	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13577	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946569	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003682	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3347	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	17	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	10	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
wcaM
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2116408..2116448 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2045 (EcoliWiki Page)	NCBI GEO profiles for wcaK
microarray	GenExpDB:b2045 (EcoliWiki Page)	Summary of data for wcaK from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to wcaK Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7096	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3347	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2045
EcoGene	EcoGene:EG13577	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003682	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006780	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	WCAK	From SHIGELLACYC
E. coli O157	WCAK	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04230 Polysaccharide pyruvyl transferase
EcoCyc	EcoCyc:G7096	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13577	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003682	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3347	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006780	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

wcaK:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to wcaK Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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