tyrT:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tyrT

Mnemonic

Tyrosine

Synonyms

ECK1226, b1231, JWR0029, Su-3, Su-4, suI_III, su_III, supC, supF, supO, tyrTalpha, su(c), suIII, supFsupO[1][2], su, supFsupO

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

27.73 minutes 

MG1655: 1286845..1286761
<gbrowseImage> name=NC_000913:1286761..1286845 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1287332..1287416
<gbrowseImage> name=NC_012967:1287332..1287416 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1175482..1175566
<gbrowseImage> name=NC_012759:1175482..1175566 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1289199..1289115
<gbrowseImage> name=NC_007779:1289115..1289199 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1327086..1327002
<gbrowseImage> name=NC_010473:1327002..1327086 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

tyrT70(OS)

ochre (UAA) suppressor

CGSC:6359

tyrT58(AS)

amber (UAG) suppressor

CGSC:4540

also known as supF58

tyrT0(OS & AS)

CGSC:6734

tyrT66(AS)

amber (UAG) suppressor

CGSC:7961

tyrT47(OS)

ochre (UAA) suppressor

CGSC:10565

tyrT91(ts, OS)

"temperature sensitive, ochre (UAA) suppressor)"

PMID:4127628[3] PMID:4887525[4]

CGSC:10566

tyrT77(AS)

amber (UAG) suppressor

CGSC:10864

tyrT181(ts, AS)

"temperature sensitive, amber (UAG) suppressor"

CGSC:10865

tyrT35(OS)

ochre (UAA) suppressor

CGSC:11293

tyrT34(AS)

amber (UAG) suppressor

CGSC:11295

tyrT666(ts, AS)

"temperature sensitive, amber (UAG) suppressor"

CGSC:19043

tyrT5888(AS)

amber (UAG) suppressor

CGSC:19045

tyrT94(AS)

amber (UAG) suppressor

CGSC:27779

tyrT9999(ts, AS)

"temperature sensitive, amber (UAG) suppressor"

CGSC:43994

tyrTA81(TS)

Growth Phenotype

carries a typ(am) mutation so it recognizes stop codons and is non-functioning at 42C in the absence of tryptophan

PMID:3330757[5] PMID:4923867[6]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[7]

Status:

Primer 1:

Primer 2:

12H9

Kohara Phage

Genobase

PMID:3038334[8]

4D8

Kohara Phage

Genobase

PMID:3038334[8]

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[9]

est. P1 cotransduction: 8% [10]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[9]

est. P1 cotransduction: 55% [10]
Synonyms:zch-506::Tn10, zci-506::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG30106

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG30106

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002548

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945820

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4269

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004136

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Beckman, D & Cooper, S (1973) Temperature-sensitive nonsense mutations in essential genes of Escherichia coli. J. Bacteriol. 116 1336-42 PubMed
  4. Reiner, AM (1969) Genetic locus for ribonuclease I in Escherichia coli. J. Bacteriol. 97 1522-3 PubMed
  5. Hussain, K et al. (1987) ParD: a new gene coding for a protein required for chromosome partitioning and septum localization in Escherichia coli. Mol. Microbiol. 1 73-81 PubMed
  6. Smith, JD et al. (1970) More mutant tyrosine transfer ribonucleic acids. J. Mol. Biol. 54 1-14 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 CGSC: The Coli Genetics Stock Center
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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