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Product(s) |

Expression|

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Standard Name	tusA
Gene Synonym(s)	yhhP, ECK3454, b3470, JW3435, tusA^[1]^[2]^[3], sirA, tusA
Product Desc.	sulfur transfer protein^[3]^[2] 2-thiolation step of mnm(5)-s(2)U34-tRNA synthesis; motility defect; affects RpoS stability; may bind RNA; mutants form filaments dues to disruption of ftsZ ring; suppressed by multicopy ftsZ or dksA; required for cell growth^[4]
Product Synonyms(s)	conserved protein required for cell growth^[1], B3470^[3]^[1], YhhP^[3]^[1], SirA^[3]^[1], TusA^[3]^[1] , TusA, ECK3454, JW3435, tusA, yhhP, b3470
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): tusA^[3]
Regulation/Activity
Quick Links
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Notes

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SirA(TusA) interacts with and activates IscS, accepting sulfur from IscS to form C19 persulfide; transfers C19 persulfide to C78 of YheN(TusD). The gene name tus has already been used as a primary gene name, so tusA-E are kept as synonyms in EcoGene.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	tusA
Mnemonic	Sigma S regulation
Synonyms	yhhP, ECK3454, b3470, JW3435, tusA^[1]^[2]^[3], sirA, tusA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	77.74 minutes	MG1655: 3607019..3606774 <gbrowseImage> name=NC_000913:3606774..3607019 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3539684..3539439 <gbrowseImage> name=NC_012967:3539439..3539684 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3495268..3495513 <gbrowseImage> name=NC_012759:3495268..3495513 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4031419..4031664 <gbrowseImage> name=NC_007779:4031419..4031664 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3704764..3704519 <gbrowseImage> name=NC_010473:3704519..3704764 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	AA change(s)	Phenotype: Description	Reference	Availability	Comments
tusAC19S	C19S	Loss of activity			seeded from UniProt:P0A890
tusAE21K	E21K	In sirA1; affects the stability of sigma-S during the logarithmic growth phase			seeded from UniProt:P0A890
tusAC56S	C56S	No effect			seeded from UniProt:P0A890
ΔyhhP725::kan			PMID:16738554	CGSC:105224
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource

Resource Type

Source

Notes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

7H7

Kohara Phage

Genobase

PMID:3038334

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12216	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12216	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002103	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947974	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2130	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011336	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	TusA
Synonyms	conserved protein required for cell growth^[1], B3470^[3]^[1], YhhP^[3]^[1], SirA^[3]^[1], TusA^[3]^[1] , TusA, ECK3454, JW3435, tusA, yhhP, b3470
Product description	sulfur transfer protein^[3]^[2] 2-thiolation step of mnm(5)-s(2)U34-tRNA synthesis; motility defect; affects RpoS stability; may bind RNA; mutants form filaments dues to disruption of ftsZ ring; suppressed by multicopy ftsZ or dksA; required for cell growth^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005515	protein binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001455	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00413	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001455	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008033	tRNA processing	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001455	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008033	tRNA processing	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0819	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016783	sulfurtransferase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00413	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016783	sulfurtransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001455	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	lacZ	PMID:16606699	Experiment(s):EBI-1145987
Protein	nadE	PMID:16606699	Experiment(s):EBI-1145987
Protein	glnA	PMID:16606699	Experiment(s):EBI-1145987
Protein	aceA	PMID:16606699	Experiment(s):EBI-1145987
Protein	mngB	PMID:16606699	Experiment(s):EBI-1145987
Protein	rho	PMID:16606699	Experiment(s):EBI-1145987
Protein	iscS	PMID:16606699	Experiment(s):EBI-1145987
Protein	prs	PMID:16606699	Experiment(s):EBI-1145987
Protein	ybaA	PMID:16606699	Experiment(s):EBI-1145987
Protein	aceE	PMID:15690043	Experiment(s):EBI-888431
Protein	iscS	PMID:15690043	Experiment(s):EBI-888431, EBI-893577
Protein	gapA	PMID:15690043	Experiment(s):EBI-893805
Protein	skp	PMID:15690043	Experiment(s):EBI-893805
Protein	rplL	PMID:15690043	Experiment(s):EBI-893805
Protein	rpoB	PMID:15690043	Experiment(s):EBI-893805
Protein	yjeE	PMID:15690043	Experiment(s):EBI-893805
Protein	skp	PMID:19402753	LCMS(ID Probability):99.6
Protein	ravA	PMID:19402753	MALDI(Z-score):27.217898
Protein	iscS	PMID:19402753	LCMS(ID Probability):99.6 MALDI(Z-score):39.615124
Protein	rpoB	PMID:19402753	LCMS(ID Probability):99.6
Protein	yjeE	PMID:19402753	LCMS(ID Probability):99.6
Protein	gapA	PMID:19402753	LCMS(ID Probability):99.6
Protein	ddpA	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplL	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTDLFSSPDH TLDALGLRCP EPVMMVRKTV RNMQPGETLL IIADDPATTR DIPGFCTFME HELVAKETDG LPYRYLIRKG G
Length	81
Mol. Wt	9.094 kDa
pI	5.1 (calculated)
Extinction coefficient	2,980 - 3,230 (calc based on 2 Y, 0 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	10..79	PF01206 SirA-like protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=sirA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131342	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947974	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011336	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A890	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12216	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12216	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947974	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002103	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2130	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	10.855+/-0.064	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.044897959	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	2167	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	287	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1251	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
tusA
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File:.jpg

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3606999..3607039 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3470 (EcoliWiki Page)	NCBI GEO profiles for sirA
microarray	GenExpDB:b3470 (EcoliWiki Page)	Summary of data for sirA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3606541..3606857) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ04; Well:A8^[5]
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Notes

Accessions Related to sirA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12216	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2130	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3470
EcoGene	EcoGene:EG12216	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002103	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011336	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000023105 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YHHP	From SHIGELLACYC
E. coli O157	YHHP	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:64307
Pfam (EcoliWiki Page)	PF01206 SirA-like protein
EcoCyc	EcoCyc:EG12216	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12216	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002103	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2130	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011336	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.00 ^3.01 ^3.02 ^3.03 ^3.04 ^3.05 ^3.06 ^3.07 ^3.08 ^3.09 ^3.10 ^3.11 ^3.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

tusA:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to sirA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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