trpS:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
trpS |
|---|---|
| Mnemonic |
Tryptophan |
| Synonyms |
ECK3371, b3384, JW3347[1], JW3347 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
75.67 minutes, 75.67 minutes |
MG1655: 3511660..3510656 |
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NC_012967: 3442320..3441316 |
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NC_012759: 3397813..3398817 |
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W3110 |
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W3110: 4126778..4127782 |
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DH10B: 3609405..3608401 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
3510659 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
trpST60R |
T60R |
(in TRPS567C; reduced activity, thermolabile) |
Strain variation; seeded from UniProt:P00954 | ||||
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trpSL91F |
L91F |
(in TRPS10330; reduced activity) |
Strain variation; seeded from UniProt:P00954 | ||||
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trpSD112E |
D112E |
(in TRPS9969; no effect in activity) |
Strain variation; seeded from UniProt:P00954 | ||||
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trpSP129S |
P129S |
(in TRPS9969; reduced activity) |
Strain variation; seeded from UniProt:P00954 | ||||
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trpSA133E |
A133E |
(in TRPS42C; reduced activity) |
Strain variation; seeded from UniProt:P00954 | ||||
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trpSM196I |
M196I |
(in TRPS271C; activity largely reduced) |
Strain variation; seeded from UniProt:P00954 | ||||
|
trpSG329S |
G329S |
(in TRPS4040; reduced activity) |
Strain variation; seeded from UniProt:P00954 | ||||
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trpS44 |
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SP361(lamGB2)(ptrpS13) |
Deletion of trpR and insertion of extra trpS gene. |
Resistant to |
There was increase resistance toward Indolmycin |
See Figure 2 for full experimental results. | |||
|
SP361(lamGB2) |
Deletion of trpR gene. |
Resistant to |
There was a slight increase in resistance toward Indolmycin. |
See Figure 2 for full experimental results. | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3347 |
Plasmid clone |
Status:Clone OK Primer 1:GCCACTAAGCCCATCGTTTTTAG Primer 2:CCtGGCTTCGCCACAAAACCAAT | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 77% [7] | ||
|
Linked marker |
est. P1 cotransduction: 8% [7] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Winter, GP et al. (1977) Sequence homologies between the tryptophanyl tRNA synthetases of Bacillus stearothermophilus and Escherichia coli. FEBS Lett. 82 348-50 PubMed
- ↑ 3.0 3.1 Bogosian, G et al. (1983) Indolmycin-mediated inhibition and stimulation of transcription at the trp promoter of Escherichia coli. J. Bacteriol. 153 1120-3 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
- ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
