PMID:6337120
| Citation |
Bogosian, G, Haydock, PV and Somerville, RL (1983) Indolmycin-mediated inhibition and stimulation of transcription at the trp promoter of Escherichia coli. J. Bacteriol. 153:1120-3 |
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| Abstract |
Escherichia coli cells harboring a non-attenuated trp-lac operon fusion were used to evaluate the effects of indolmycin on the initiation of transcription at the trp promoter. Indolmycin caused repression in trpR+ strains and in trpR deletion mutants, although higher effector concentrations were required in the latter situation. Plasmid-mediated elevation in tryptophanyl-tRNA synthetase reversed the inhibitory effect of indolmycin. Indolmycin did not facilitate the binding of purified Trp repressor protein to trp operator DNA. |
| Links | |
| Keywords |
Bacterial Proteins; DNA, Bacterial/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Indoles/pharmacology; Mutation; Operon; Repressor Proteins/metabolism; Transcription, Genetic/drug effects; Tryptophan/biosynthesis; Tryptophan-tRNA Ligase/metabolism |
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