trpR:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
trpR |
|---|---|
| Mnemonic |
Tryptophan |
| Synonyms |
ECK4385, b4393, JW4356, rtrY[1] |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
99.81 minutes |
MG1655: 4630783..4631109 |
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NC_012967: 4620973..4621299 |
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NC_012759: 4569267..4569593 |
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W3110 |
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W3110: 4637440..4637766 |
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DH10B: 4677245..4677571 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
4630786 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
trpR(del) (Keio:JW4356) |
deletion |
deletion |
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trpR55 |
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trpR72(Am) |
amber (UAG) mutation | ||||||
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trpR80 |
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trpR47 |
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trpR789(del)::kan |
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SP361(lamGB2)(ptrpS13) |
Deletion of trpR and insertion of extra trpS gene. |
Resistant to |
Increase resistance toward Indolmycin. |
See figure 2 for experimental results. | |||
|
SP361(lamGB2) |
Deletion of trpR gene. |
Resistant to |
Increase resistance toward Indolmycin |
See figure 2 for full experimental results. | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW4356 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGCCCAACAATCACCCTATTC Primer 2:CCATCGCTTTTCAGCAACACCTC | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 21% [11] | ||
|
Linked marker |
est. P1 cotransduction: 74% [11] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Tsapakos, MJ et al. (1985) Ligand-mediated conformational changes in Trp repressor protein of Escherichia coli probed through limited proteolysis and the use of specific antibodies. J. Biol. Chem. 260 16383-94 PubMed
- ↑ Joachimiak, A et al. (1983) Purification and characterization of trp aporepressor. Proc. Natl. Acad. Sci. U.S.A. 80 668-72 PubMed
- ↑ Gunsalus, RP & Yanofsky, C (1980) Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor. Proc. Natl. Acad. Sci. U.S.A. 77 7117-21 PubMed
- ↑ 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
- ↑ Yanofsky, C et al. (1984) Repression is relieved before attenuation in the trp operon of Escherichia coli as tryptophan starvation becomes increasingly severe. J. Bacteriol. 158 1018-24 PubMed
- ↑ 8.0 8.1 Bogosian, G et al. (1983) Indolmycin-mediated inhibition and stimulation of transcription at the trp promoter of Escherichia coli. J. Bacteriol. 153 1120-3 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 10.0 10.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
