tolA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tolA

Mnemonic

Tolerance

Synonyms

ECK0728, b0739, JW0729, cim, excC, lky, tol-2[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

16.72 minutes 

MG1655: 775565..776830
<gbrowseImage> name=NC_000913:775565..776830 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 757224..758489
<gbrowseImage> name=NC_012967:757224..758489 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 678325..679590
<gbrowseImage> name=NC_012759:678325..679590 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 776764..778029
<gbrowseImage> name=NC_007779:776764..778029 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 828157..829422
<gbrowseImage> name=NC_010473:828157..829422 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

tolA(del) (Keio:JW0729)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8795[3]

tolA::Tn5KAN-2 (FB20229)

Insertion at nt 197 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20229

does not contain pKD46

tolA1

CGSC:7864

tolA788(del)::kan

PMID:16738554[2]

CGSC:103162

tolA mutation in A592

Resistant to

Resistance to Colicins E1 & E2

PMID:3001021[5]

A mutation to the tolA gene conferred resistance to the Colicins E1 and E2.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0729

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCAAAGGCAACCGAACAAAA

Primer 2:CCCGGTTTGAAGTCCAATGGCGC

18F11

Kohara Phage

Genobase

PMID:3038334[7]

10G5

Kohara Phage

Genobase

PMID:3038334[7]

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[3]

est. P1 cotransduction: 1% [8]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[3]

est. P1 cotransduction: 88% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11007

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11007

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000996

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946625

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1000

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002515

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Sun, TP & Webster, RE (1986) fii, a bacterial locus required for filamentous phage infection and its relation to colicin-tolerant tolA and tolB. J. Bacteriol. 165 107-15 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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