tnaC:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tnaC

Mnemonic

Tryptophanase

Synonyms

ECK3700, b3707, JW3685, tnaL[1], tnaL

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

83.77 minutes 

MG1655: 3886458..3886532
<gbrowseImage> name=NC_000913:3886458..3886532 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3848796..3848870
<gbrowseImage> name=NC_012967:3848796..3848870 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3774791..3774865
<gbrowseImage> name=NC_012759:3774791..3774865 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3751980..3751906
<gbrowseImage> name=NC_007779:3751906..3751980 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3984042..3984116
<gbrowseImage> name=NC_010473:3984042..3984116 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

tnaC(del) (Keio:JW3685)

deletion

deletion

PMID:16738554[2]

Shigen

CGSC10694[3]

tnaC-738(del)::kan

PMID:16738554[2]

CGSC:103160

tnaC(del)

deletion

deletion

motility

Increased motility compared to wild-type strain, figure 6.

PMID:17511876[4]

tnaC(del)

deletion

deletion

Intracellular metabolites

Decreased intracellular indole (10% of wild-type levels)-Fig. 2

PMID:17511876[4]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3685

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAATATCTTACATATATGTGT

Primer 2:CCAGGGCGGTGATCGACAATTTT

2A2

Kohara Phage

Genobase

PMID:3038334[6]

3F11

Kohara Phage

Genobase

PMID:3038334[6]

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[3]

est. P1 cotransduction: 65% [7]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[3]

est. P1 cotransduction: 15% [7]
Synonyms:zie-296::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11276

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11276

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001250

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948223

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1254

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012128

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 Lee, J et al. (2007) Indole is an inter-species biofilm signal mediated by SdiA. BMC Microbiol. 7 42 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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