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Expression|

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Standard Name	srlB
Gene Synonym(s)	ECK2699, b2704, JW2673, gutB^[1], gutB
Product Desc.	glucitol/sorbitol-specific enzyme IIA component of PTS^[2]^[3]; Component of glucitol/sorbitol PTS permease^[2]^[3] D-glucitol (sorbitol)-specific enzyme III of the phosphotransferase system^[4]
Product Synonyms(s)	glucitol/sorbitol-specific enzyme IIA component of PTS^[1], B2704^[2]^[1], GutB^[2]^[1], SrlB^[2]^[1] , ECK2699, gutB, JW2673, b2704
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): srlAEBD-gutM-srlR-gutQ^[2], OP00177, gutABDMR, srlABDMR
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	srlB
Mnemonic	Sorbitol
Synonyms	ECK2699, b2704, JW2673, gutB^[1], gutB
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	60.9 minutes	MG1655: 2825384..2825755 <gbrowseImage> name=NC_000913:2825384..2825755 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2722221..2722592 <gbrowseImage> name=NC_012967:2722221..2722592 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2711196..2711567 <gbrowseImage> name=NC_012759:2711196..2711567 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2826018..2826389 <gbrowseImage> name=NC_007779:2826018..2826389 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2917926..2918297 <gbrowseImage> name=NC_010473:2917926..2918297 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
srlB(del) (Keio:JW2673)	deletion	deletion		PMID:16738554	Shigen CGSC10108^[5]
srlBI17T		I17T	(in strain: ECOR 6, ECOR 28, ECOR 35, ECOR 37, ECOR 51, ECOR 58, ECOR 61, ECOR 66 and ECOR 69)			Strain variation; seeded from UniProt:P05706
srlBA51E		A51E	(in strain: ECOR 58)			Strain variation; seeded from UniProt:P05706
srlBS59T		S59T	(in strain: ECOR 28, ECOR 37, ECOR 58 and ECOR 69)			Strain variation; seeded from UniProt:P05706
srlBQ62R		Q62R	(in strain: ECOR 51)			Strain variation; seeded from UniProt:P05706
srlBN92S		N92S	(in strain: ECOR 51, ECOR 61 and ECOR 66)			Strain variation; seeded from UniProt:P05706
srlB779(del)::kan				PMID:16738554	CGSC:103012
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2673	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACCGTTATTTATCAGACCAC Primer 2:CCtTCCTTAACAGATTCAAACTT
23B7	Kohara Phage	Genobase	PMID:3038334
9G10	Kohara Phage	Genobase	PMID:3038334
pheA18::Tn10	Linked marker	CAG12158 = CGSC7421^[5]	est. P1 cotransduction: % ^[6]
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[5]	est. P1 cotransduction: 99% ^[6] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10970	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10970	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000959	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948971	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0963	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008894	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	SrlB
Synonyms	glucitol/sorbitol-specific enzyme IIA component of PTS^[1], B2704^[2]^[1], GutB^[2]^[1], SrlB^[2]^[1] , ECK2699, gutB, JW2673, b2704
Product description	glucitol/sorbitol-specific enzyme IIA component of PTS^[2]^[3]; Component of glucitol/sorbitol PTS permease^[2]^[3] D-glucitol (sorbitol)-specific enzyme III of the phosphotransferase system^[4]
EC number (for enzymes)	2.7.1.69^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004716	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018454	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008643	carbohydrate transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0762	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008982	protein-N(PI)-phosphohistidine-sugar phosphotransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004716	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008982	protein-N(PI)-phosphohistidine-sugar phosphotransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018454	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004716	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018454	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0598	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016301	kinase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0418	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of glucitol/sorbitol PTS permease	could be indirect
Protein	gsp		PMID:16606699	Experiment(s):EBI-1143708
Protein	arnA		PMID:16606699	Experiment(s):EBI-1143708
Protein	ydcP		PMID:16606699	Experiment(s):EBI-1143708
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTVIYQTTIT RIGASAIDAL SDQMLITFRE GAPADLEEYC FIHCHGELKG ALHPGLQFSL GQHRYPVTAV GSVAEDNLRE LGHVTLRFDG LNEAEFPGTV HVAGPVPDDI APGSVLKFES VKE
Length	123
Mol. Wt	13.303 kDa
pI	4.8 (calculated)
Extinction coefficient	4,470 - 4,720 (calc based on 3 Y, 0 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..118	PF03829 PTS system glucitol/sorbitol-specific IIA component		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=srlB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130611	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948971	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008894	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P05706	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10970	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10970	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948971	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000959	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0963	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	83	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	28	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	78^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
srlAEBD-gutM-srlR-gutQ
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2825364..2825404 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2704 (EcoliWiki Page)	NCBI GEO profiles for srlB
microarray	GenExpDB:b2704 (EcoliWiki Page)	Summary of data for srlB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to srlB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10970	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0963	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2704
EcoGene	EcoGene:EG10970	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000959	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008894	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	SRLB	From SHIGELLACYC
E. coli O157	SRLB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:141530
Pfam (EcoliWiki Page)	PF03829 PTS system glucitol/sorbitol-specific IIA component
EcoCyc	EcoCyc:EG10970	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10970	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000959	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0963	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008894	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

srlB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to srlB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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