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Standard Name	solA
Gene Synonym(s)	ECK1044, b1059, JW1046^[1], JW1046
Product Desc.	N-methyltryptophan oxidase, FAD-containing; monomeric^[2]
Product Synonyms(s)	N-methyltryptophan oxidase, FAD-binding^[1], B1059^[3]^[1], SolA^[3]^[1] , ECK1044, JW1046, b1059
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yceO^[3]
Regulation/Activity
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Notes

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SolA has weak activity on carbinolamines, sarcosine and other secondary amino acids. True physiological substrate is unknown. Cys308 is the probable FAD attachment site. Mass spectral analysis gives an observed MW of 41684 daltons. FAD-SolA holoenzyme MW is predicted as 41688, indicating the sequence and start codon is correct and that the initial Met is not clipped.^[2]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	solA
Mnemonic	Sarcosine oxidase-like
Synonyms	ECK1044, b1059, JW1046^[1], JW1046
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	24.11 minutes	MG1655: 1119809..1118691 <gbrowseImage> name=NC_000913:1118691..1119809 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1135155..1134037 <gbrowseImage> name=NC_012967:1134037..1135155 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1022616..1023734 <gbrowseImage> name=NC_012759:1022616..1023734 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1122163..1121045 <gbrowseImage> name=NC_007779:1121045..1122163 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1175254..1174136 <gbrowseImage> name=NC_010473:1174136..1175254 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔsolA (Keio:JW1046)	deletion	deletion	PMID:16738554	Shigen CGSC9008^[4]
ΔsolA729::kan			PMID:16738554	CGSC:102988
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1046	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAATACGATCTCATCATTAT Primer 2:CCTTGGAAGCGGGAAAGCCTGAA
1H7	Kohara Phage	Genobase	PMID:3038334
E4H10S	Kohara Phage	Genobase	PMID:3038334
zcc-282::Tn10	Linked marker	CAG18466 = CGSC7359^[4]	est. P1 cotransduction: 6% ^[5]
zce-726::Tn10	Linked marker	CAG12078 = CGSC7361^[4]	est. P1 cotransduction: 22% ^[5]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6556	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12669	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003152	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944983	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2535	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003590	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	SolA
Synonyms	N-methyltryptophan oxidase, FAD-binding^[1], B1059^[3]^[1], SolA^[3]^[1] , ECK1044, JW1046, b1059
Product description	N-methyltryptophan oxidase, FAD-containing; monomeric^[2]
EC number (for enzymes)	1.5.3.2^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	PMID:11967071	IEP: Inferred from Expression Pattern		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	purC	PMID:16606699	Experiment(s):EBI-1138724
Protein	yjdA	PMID:16606699	Experiment(s):EBI-1138724
Protein	leuS	PMID:16606699	Experiment(s):EBI-1138724
Protein	rmuC	PMID:16606699	Experiment(s):EBI-1138724
Protein	yibA	PMID:16606699	Experiment(s):EBI-1138724
Protein	nrfA	PMID:16606699	Experiment(s):EBI-1138724
Protein	envZ	PMID:16606699	Experiment(s):EBI-1138724
Protein	rplB	PMID:16606699	Experiment(s):EBI-1138724
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKYDLIIIGS GSVGAAAGYY ATRAGLNVLM TDAHMPPHQH GSHHGDTRLI RHAYGEGEKY VPLVLRAQTL WDELSRHNEE DPIFVRSGVI NLGPADSTFL ANVAHSAEQW QLNVEKLDAQ GIMARWPEIR VPDNYIGLFE TDSGFLRSEL AIKTWIQLAK EAGCAQLFNC PVTAIRHDDD GVTIETADGE YQAKKAIVCA GTWVKDLLPE LPVQPVRKVF AWYQADGRYS VKNKFPAFTG ELPNGDQYYG FPAENDALKI GKHNGGQVIH SADERVPFAE VASDGSEAFP FLRNVLPGIG CCLYGAACTY DNSPDEDFII DTLPGHDNTL LITGLSGHGF KFASVLGEIA ADFAQDKKSD FDLTPFRLSR FQ
Length	372
Mol. Wt	40.902 kDa
pI	5.1 (calculated)
Extinction coefficient	52,370 - 53,120 (calc based on 13 Y, 6 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	4..353	PF01266 FAD dependent oxidoreductase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=solA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129022	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944983	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003590	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P40874	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6556	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12669	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944983	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003152	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2535	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	3.01E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	1014	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	800	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	784	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yceO
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1119789..1119829 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1059 (EcoliWiki Page)	NCBI GEO profiles for solA
microarray	GenExpDB:b1059 (EcoliWiki Page)	Summary of data for solA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1119739..1119969) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ07; Well:C12^[6]
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Notes

Accessions Related to solA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6556	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2535	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1059
EcoGene	EcoGene:EG12669	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003152	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003590	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT2G24580 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000010444 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00027620 (score: 1.000; bootstrap: 100%) WBGene00027621 (score: 0.590)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00015788 (score: 1.000; bootstrap: 100%) WBGene00015783 (score: 0.570)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000027853 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000020711 (score: 1.000; bootstrap: 100%) ENSCINP00000012361 (score: 0.181) ENSCINP00000006592 (score: 0.172)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0215209 (score: 1.000; bootstrap: 91%)	From Inparanoid:20070104
Homo sapiens	ENSP00000317721 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000012999 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000024114 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:1197006 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000015268 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000012154 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC3545 (score: 1.000; bootstrap: 100%) SPAC1394c (score: 0.161)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000130406 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00008761001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000020868 (score: 1.000; bootstrap: 98%)	From Inparanoid:20070104
Shigella flexneri	SOLA	From SHIGELLACYC
E. coli O157	SOLA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01266 FAD dependent oxidoreductase
Superfamily (EcoliWiki Page)	SUPERFAMILY:51905
Panther (EcoliWiki Page)	PTHR10961:SF7
Superfamily (EcoliWiki Page)	SUPERFAMILY:54373
EcoCyc	EcoCyc:G6556	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12669	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003152	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2535	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003590	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

solA:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to solA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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