shiA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

shiA

Gene Synonym(s)

ECK1976, b1981, JW1962, yeeM[1], yeeM

Product Desc.

ShiA shikimate MFS transporter[2][3]

Product Synonyms(s)

shikimate transporter[1], B1981[2][1], YeeM[2][1], ShiA[2][1] , ECK1976, JW1962, yeeM, b1981

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): shiA[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

shiA

Mnemonic

Shikimate

Synonyms

ECK1976, b1981, JW1962, yeeM[1], yeeM

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

44.22 minutes 

MG1655: 2051667..2052983
<gbrowseImage> name=NC_000913:2051667..2052983 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1991642..1992958
<gbrowseImage> name=NC_012967:1991642..1992958 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1944150..1945466
<gbrowseImage> name=NC_012759:1944150..1945466 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2055780..2057096
<gbrowseImage> name=NC_007779:2055780..2057096 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2142675..2143991
<gbrowseImage> name=NC_010473:2142675..2143991 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔshiA (Keio:JW1962)

deletion

deletion

PMID:16738554

Shigen
CGSC9627[4]

shiA352

CGSC:11304

ΔshiA755::kan

PMID:16738554

CGSC:102954


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1962

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGACTCCACGCTCATCTCCAC

Primer 2:CCAGCGCGTTGACTGTCTTTCAT

7D4

Kohara Phage

Genobase

PMID:3038334

9B2

Kohara Phage

Genobase

PMID:3038334

zed-3069::Tn10

Linked marker

CAG18451 = CGSC7395[4]

est. P1 cotransduction: 59% [5]

zef-3129::Tn10

Linked marker

CAG12099 = CGSC7397[4]

est. P1 cotransduction: 29% [5]
Synonyms:zee-3129::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7067

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20205

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003653

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946495

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4148

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006572

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ShiA

Synonyms

shikimate transporter[1], B1981[2][1], YeeM[2][1], ShiA[2][1] , ECK1976, JW1962, yeeM, b1981

Product description

ShiA shikimate MFS transporter[2][3]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005215

transporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004736

F

Seeded from EcoCyc (v14.0)

complete

GO:0005215

transporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005828

F

Seeded from EcoCyc (v14.0)

complete

GO:0005215

transporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005829

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004736

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005828

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005829

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0015293

symporter activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0769

F

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005829

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004736

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005828

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0055085

transmembrane transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005828

P

Seeded from EcoCyc (v14.0)

complete

GO:0055085

transmembrane transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005829

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 12 predicted transmembrane domains

Daley et al. (2005) [6]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MDSTLISTRP DEGTLSLSRA RRAALGSFAG AVVDWYDFLL YGITAALVFN REFFPQVSPA
MGTLAAFATF GVGFLFRPLG GVIFGHFGDR LGRKRMLMLT VWMMGIATAL IGILPSFSTI
GWWAPILLVT LRAIQGFAVG GEWGGAALLS VESAPKNKKA FYSSGVQVGY GVGLLLSTGL
VSLISMMTTD EQFLSWGWRI PFLFSIVLVL GALWVRNGME ESAEFEQQQH YQAAAKKRIP
VIEALLRHPG AFLKIIALRL CELLTMYIVT AFALNYSTQN MGLPRELFLN IGLLVGGLSC
LTIPCFAWLA DRFGRRRVYI TGTLIGTLSA FPFFMALEAQ SIFWIVFFSI MLANIAHDMV
VCVQQPMFTE MFGASYRYSG AGVGYQVASV VGGGFTPFIA AALITYFAGN WHSVAIYLLA
GCLISAMTAL LMKDSQRA
Length

438

Mol. Wt

47.818 kDa

pI

9.4 (calculated)

Extinction coefficient

79,870 - 80,495 (calc based on 13 Y, 11 W, and 5 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

23..433

PF00083 Sugar (and other) transporter

PMID:19920124

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<motif_map/>

tmhmm.php?gene=shiA&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=shiA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129925

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946495

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006572

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P76350

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G7067

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20205

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946495

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003653

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4148

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

163

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

163

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

245

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

shiA

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2051647..2051687 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1981 (EcoliWiki Page)

NCBI GEO profiles for shiA

microarray

GenExpDB:b1981 (EcoliWiki Page)

Summary of data for shiA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (2051074..2051495) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ16; Well:G7[7]

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Notes

Accessions Related to shiA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7067

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4148

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1981

EcoGene

EcoGene:EG20205

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003653

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006572

Escherichia coli str. K-12 substr. MG1655

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Caenorhabditis briggsae

  • WBGene00039162 (score: 1.000; bootstrap: 100%)
  • WBGene00027098 (score: 0.160)
  • WBGene00024393 (score: 0.149)
  • WBGene00027474 (score: 0.140)
  • WBGene00025704 (score: 0.139)
  • WBGene00024395 (score: 0.135)
  • WBGene00032394 (score: 0.133)
  • WBGene00024452 (score: 0.120)
  • WBGene00042042 (score: 0.119)
  • WBGene00030924 (score: 0.116)
  • WBGene00039573 (score: 0.116)
  • WBGene00026048 (score: 0.110)
  • WBGene00024396 (score: 0.109)
  • WBGene00027974 (score: 0.109)
  • WBGene00027345 (score: 0.105)
  • WBGene00032396 (score: 0.104)
  • WBGene00029653 (score: 0.104)
  • WBGene00032547 (score: 0.097)
  • WBGene00031157 (score: 0.081)
  • WBGene00041186 (score: 0.080)
  • WBGene00038285 (score: 0.076)
  • WBGene00039083 (score: 0.074)
  • WBGene00037407 (score: 0.072)
  • WBGene00041731 (score: 0.070)
  • WBGene00033157 (score: 0.066)
  • WBGene00024723 (score: 0.064)
  • WBGene00039084 (score: 0.056)
  • WBGene00024312 (score: 0.053)
  • WBGene00039082 (score: 0.052)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00022348 (score: 1.000; bootstrap: 100%)
  • WBGene00009729 (score: 0.309)
  • WBGene00003842 (score: 0.131)
  • WBGene00013977 (score: 0.091)
  • WBGene00019408 (score: 0.056)

From Inparanoid:20070104

E. coli O157

SHIA

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:103473

Pfam (EcoliWiki Page)

PF00083 Sugar (and other) transporter

EcoCyc

EcoCyc:G7067

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20205

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003653

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4148

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006572

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  7. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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