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Standard Name	sfsA
Gene Synonym(s)	ECK0145, b0146, JW0142, sfs1, sfs^[1], sfs
Product Desc.	predicted DNA-binding transcriptional regulator of maltose metabolism^[2]^[3] Overexpression increases maltose utilization; stimulates MalQ^[4]
Product Synonyms(s)	predicted DNA-binding transcriptional regulator^[1], B0146^[2]^[1], Sfs^[2]^[1], SfsA^[2]^[1] , ECK0145, JW0142, sfs, sfs1, b0146
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): sfsA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	sfsA
Mnemonic	sugar fermentation stimulation Sugar fermentation stimulation
Synonyms	ECK0145, b0146, JW0142, sfs1, sfs^[1], sfs
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Notes

The gene was named sfs because when present in multicopy it allows a ∆cya crp*1 mutant to ferment maltose ^[5]. Whether it is normally involved in maltose catabolism has not been determined.

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	3.47 minutes	MG1655: 161486..160782 <gbrowseImage> name=NC_000913:160782..161486 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 164337..163633 <gbrowseImage> name=NC_012967:163633..164337 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 160781..161485 <gbrowseImage> name=NC_012759:160781..161485 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 161486..160782 <gbrowseImage> name=NC_007779:160782..161486 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 135590..134886 <gbrowseImage> name=NC_010473:134886..135590 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
sfsA(del) (Keio:JW0142)	deletion	deletion	PMID:16738554	Shigen CGSC8413^[6]
sfsA762(del)::kan			PMID:16738554	CGSC:102934
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0142	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGAATTTTCTCCCCCTCTACA Primer 2:CCCAATGTAACCGGCAGTGATTT
11C5	Kohara Phage	Genobase	PMID:3038334
15A7	Kohara Phage	Genobase	PMID:3038334
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[6]	est. P1 cotransduction: 65% ^[7]
yafC502::Tn10	Linked marker	CAG18436 = CGSC7328^[6]	est. P1 cotransduction: 1% ^[7] Synonyms:zae-502::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10949	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10949	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000938	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944855	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0942	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000503	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	SfsA
Synonyms	predicted DNA-binding transcriptional regulator^[1], B0146^[2]^[1], Sfs^[2]^[1], SfsA^[2]^[1] , ECK0145, JW0142, sfs, sfs1, b0146
Product description	predicted DNA-binding transcriptional regulator of maltose metabolism^[2]^[3] Overexpression increases maltose utilization; stimulates MalQ^[4]
EC number (for enzymes)
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Notes

When present in multicopy the sfsA gene allows a ∆cya crp*1 strain, but not a ∆cya crp⁺ strain, to ferment maltose ^[5]. A total of 12 different E. coli DNA fragments with this phenotype were isolated ^[5]. One other plasmid insert was sequenced and named sfs7 ^[8]. The sfs7 gene was later renamed sfsB. Whether sfsA and/or sfsB are normally involved in regulating maltose catabolism has not been demonstrated.

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	slyD	PMID:16606699	Experiment(s):EBI-1135713
Protein	gspJ	PMID:16606699	Experiment(s):EBI-1135713
Protein	ybiH	PMID:16606699	Experiment(s):EBI-1135713
Protein	rplC	PMID:16606699	Experiment(s):EBI-1135713
Protein	rpsD	PMID:16606699	Experiment(s):EBI-1135713
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MEFSPPLQRA TLIQRYKRFL ADVITPDGRE LTLHCPNTGA MTGCATPGDT VWYSTSDNTK RKYPHTWELT QSQSGAFICV NTLWANRLTK EAILNESISE LSGYSSLKSE VKYGAERSRI DFMLQADSRP DCYIEVKSVT LAENEQGYFP DAVTERGQKH LRELMSVAAE GQRAVIFFAV LHSAITRFSP ARHIDEKYAQ LLSEAQQRGV EILAYKAEIS AEGMALKKSL PVTL
Length	234
Mol. Wt	26.228 kDa
pI	10.5 (calculated)
Extinction coefficient	29,910 - 30,410 (calc based on 9 Y, 3 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	13..231	PF03749 Sugar fermentation stimulation protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=sfsA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128139	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944855	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000503	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A823	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10949	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10949	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944855	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000938	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0942	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	349	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	126	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	231	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
sfsA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:161466..161506 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0146 (EcoliWiki Page)	NCBI GEO profiles for sfsA
microarray	GenExpDB:b0146 (EcoliWiki Page)	Summary of data for sfsA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to sfsA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10949	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0942	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0146
EcoGene	EcoGene:EG10949	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000938	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000503	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	SFSA	From SHIGELLACYC
E. coli O157	SFSA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Among the bacteria, homologs of SfsA have been identified in members of the following phyla: Cyanobacteria, Aquificae, Thermotogae, Deinococcus-thermus, Proteobacteria, Chloroflexi, and the Firmucutes (Pfam accession PF03749).

In the Archaea, SfsA homologs have been found among the Crenarchaeota and the Euryarchaeota (Pfam accession PF03749).

Families

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Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03749 Sugar fermentation stimulation protein
InterPro	IPR005224
EcoCyc	EcoCyc:EG10949	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10949	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000938	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0942	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000503	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 Kawamukai, M et al. (1991) Nucleotide sequence and characterization of the sfs1 gene: sfs1 is involved in CRP*-dependent mal gene expression in Escherichia coli. J. Bacteriol. 173 2644-8 PubMed
↑ ^6.0 ^6.1 ^6.2 CGSC: The Coli Genetics Stock Center
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Choi, YL et al. (1989) Cloning and sequencing of an Escherichia coli gene, nlp, highly homologous to the ner genes of bacteriophages Mu and D108. J. Bacteriol. 171 5222-5 PubMed

sfsA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to sfsA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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