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Expression|

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Standard Name	rraA
Gene Synonym(s)	ECK3921, b3929, JW3900, menG, yiiA, yiiV^[1], yiiV
Product Desc.	ribonuclease E inhibitor protein^[2]^[3]; Component of ribonuclease E inhibitor protein A^[3] Protein inhibitor of RNase E, modulates global mRNA abundance; trimeric^[4]
Product Synonyms(s)	ribonuclease E (RNase E) inhibitor protein^[1], B3929^[2]^[1], YiiV^[2]^[1], MenG^[2]^[1], RraA^[2]^[1] , ECK3921, JW3900, menG, yiiV, b3929
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): rraA^[2]
Regulation/Activity
Quick Links
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Notes

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rraA (menG) was previously, erroneously, reported to encode demethylmenaquinone methyltransferase, in Genbank U56082. UbiE performs this final step, converting demethylmenaquinone to menaquinone.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	rraA
Mnemonic	Regulator of Ribonuclease Activity
Synonyms	ECK3921, b3929, JW3900, menG, yiiA, yiiV^[1], yiiV
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	88.73 minutes	MG1655: 4117353..4116868 <gbrowseImage> name=NC_000913:4116868..4117353 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 4006537..4007022 <gbrowseImage> name=NC_012759:4006537..4007022 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3517351..3517836 <gbrowseImage> name=NC_007779:3517351..3517836 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4217050..4216565 <gbrowseImage> name=NC_010473:4216565..4217050 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔrraA (Keio:JW3900)	deletion	deletion	PMID:16738554	Shigen CGSC10815^[5]
ΔrraA788::kan			PMID:16738554	CGSC:102800
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3900	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAATACGATACTTCCGAGCT Primer 2:CCTTCAATATCCAGCGGATCTTC
12E3	Kohara Phage	Genobase	PMID:3038334
4H12	Kohara Phage	Genobase	PMID:3038334
zih-35::Tn10	Linked marker	CAG18495 = CGSC7467^[5]	est. P1 cotransduction: 8% ^[6]
pflD501::Tn10	Linked marker	CAG18477 = CGSC7470^[5]	est. P1 cotransduction: 37% ^[6] Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11879	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11879	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001809	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948419	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1825	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012837	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	RraA
Synonyms	ribonuclease E (RNase E) inhibitor protein^[1], B3929^[2]^[1], YiiV^[2]^[1], MenG^[2]^[1], RraA^[2]^[1] , ECK3921, JW3900, menG, yiiV, b3929
Product description	ribonuclease E inhibitor protein^[2]^[3]; Component of ribonuclease E inhibitor protein A^[3] Protein inhibitor of RNase E, modulates global mRNA abundance; trimeric^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0008428	ribonuclease inhibitor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010203	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008428	ribonuclease inhibitor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR014339	F	Seeded from EcoCyc (v14.0)	complete
	GO:0051252	regulation of RNA metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010203	P	Seeded from EcoCyc (v14.0)	complete
	GO:0051252	regulation of RNA metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR014339	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of ribonuclease E inhibitor protein A	could be indirect
Protein	iscS		PMID:16606699	Experiment(s):EBI-1147078
Protein	yehH		PMID:16606699	Experiment(s):EBI-1147078
Protein	ahpF		PMID:16606699	Experiment(s):EBI-1147078
Protein	yrbF		PMID:16606699	Experiment(s):EBI-1147078
Protein	yegQ		PMID:16606699	Experiment(s):EBI-1147078
Protein	yehD		PMID:16606699	Experiment(s):EBI-1147078
Protein	btuF		PMID:16606699	Experiment(s):EBI-1147078
Protein	rplQ		PMID:16606699	Experiment(s):EBI-1147078
Protein	yeaH		PMID:19402753	LCMS(ID Probability):99.6
Protein	yfiF		PMID:19402753	LCMS(ID Probability):99.4
Protein	rluB		PMID:19402753	LCMS(ID Probability):99.6
Protein	ybeZ		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKYDTSELCD IYQEDVNVVE PLFSNFGGRA SFGGQIITVK CFEDNGLLYD LLEQNGRGRV LVVDGGGSVR RALVDAELAR LAVQNEWEGL VIYGAVRQVD DLEELDIGIQ AMAAIPVGAA GEGIGESDVR VNFGGVTFFS GDHLYADNTG IILSEDPLDI E
Length	161
Mol. Wt	17.359 kDa
pI	3.9 (calculated)
Extinction coefficient	12,950 - 13,200 (calc based on 5 Y, 1 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..153	PF03737 Demethylmenaquinone methyltransferase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rraA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131767	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948419	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012837	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A8R0	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11879	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11879	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948419	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001809	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1825	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	7.63E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	1.387+/-0.023	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
Protein	E. coli K-12 MG1655	3341	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1630	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1055	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
rraA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4117333..4117373 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3929 (EcoliWiki Page)	NCBI GEO profiles for rraA
microarray	GenExpDB:b3929 (EcoliWiki Page)	Summary of data for rraA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4116828..4117008) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ07; Well:B1^[7]
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Notes

Accessions Related to rraA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11879	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1825	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3929
EcoGene	EcoGene:EG11879	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001809	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012837	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT3G02770 (score: 1.000; bootstrap: 100%) AT5G16450 (score: 1.000; bootstrap: 100%) AT5G56260 (score: 0.518)	From Inparanoid:20070104
Oryza gramene	Q6Z6G8 (score: 1.000; bootstrap: 100%) Q6Z6H0 (score: 1.000; bootstrap: 100%) Q8W0C5 (score: 0.962)	From Inparanoid:20070104
Shigella flexneri	MENG	From SHIGELLACYC
E. coli O157	MENG	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03737 Demethylmenaquinone methyltransferase
Superfamily (EcoliWiki Page)	SUPERFAMILY:89562
EcoCyc	EcoCyc:EG11879	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11879	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001809	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1825	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012837	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

rraA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to rraA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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