rpsS:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rpsS

Mnemonic

Ribosomal protein, small

Synonyms

ECK3303, b3316, JW3278[1], JW3278

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.32 minutes, 74.32 minutes 

MG1655: 3448548..3448270
<gbrowseImage> name=NC_000913:3448270..3448548 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3378431..3378153
<gbrowseImage> name=NC_012967:3378153..3378431 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3335427..3335705
<gbrowseImage> name=NC_012759:3335427..3335705 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4189890..4190168
<gbrowseImage> name=NC_007779:4189890..4190168 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3546293..3546015
<gbrowseImage> name=NC_010473:3546015..3546293 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3448273

Edman degradation

PMID:348496[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rpsSH83Y

H83Y

(in MW145; suppresses a rimM deletion)

Strain variation; seeded from UniProt:P0A7U3

rpsS in strain TS14-13-10

Resistant to

Three step tiamulin resistance

PMID:372756[3]

Strain: TS14-13-10

in table 1 and 2

rpsS6

Resistant to

Resistance to gentamicin.

PMID:342908[4]

GE20-6


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3278

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCCCACGTTCTCTCAAGAAAGG

Primer 2:CCTTTCTTCTTCGCTTTTTTATC

E1H9

Kohara Phage

Genobase

PMID:3038334[6]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[7]

est. P1 cotransduction: 49% [8]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[7]

est. P1 cotransduction: 7% [8]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10918

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10918

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000909

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947811

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0911

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010852

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Yaguchi, M & Wittmann, HG (1978) Primary structure of protein S19 from the small ribosomal subunit of Escherichia coli. FEBS Lett. 88 227-30 PubMed
  3. Hummel, H et al. (1979) Analysis of lincomycin resistance mutations in Escherichia coli. Mol. Gen. Genet. 169 345-7 PubMed
  4. Buckel, P et al. (1977) Alteration of ribosomal protein L6 in mutants of Escherichia coli resistant to gentamicin. Mol. Gen. Genet. 158 47-54 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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