rpsG:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rpsG

Mnemonic

Ribosomal protein, small

Synonyms

ECK3328, b3341, JW3303, K12[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.82 minutes 

MG1655: 3472103..3471564
<gbrowseImage> name=NC_000913:3471564..3472103 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3401986..3401516
<gbrowseImage> name=NC_012967:3401516..3401986 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3358721..3359260
<gbrowseImage> name=NC_012759:3358721..3359260 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4166335..4166874
<gbrowseImage> name=NC_007779:4166335..4166874 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3569848..3569309
<gbrowseImage> name=NC_010473:3569309..3569848 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3471567

Edman degradation

PMID:385062[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rpsG

Deletion

rpsG

Deletion

rpsGK36A,E

K36A,E

Defective in ribosome assembly

seeded from UniProt:P02359

rpsGM116G

M116G

Significantly decreases affinity for its own mRNA

seeded from UniProt:P02359

rpsG in strain LE2-10

Resistant to

Resistant to lincomycin

PMID:372756[3]

Strain: LE2-10

figure 2

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3303

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCCCACGTCGTCGCGTCATTGG

Primer 2:CCATTTAAGTAGCCCAAAGCGGG

3F8

Kohara Phage

Genobase

PMID:3038334[5]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[6]

est. P1 cotransduction: 16% [7]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[6]

est. P1 cotransduction: 29% [7]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10906

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10906

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000897

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947846

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0899

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010917

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Reinbolt, J et al. (1979) The primary structure of ribosomal protein S7 from E. coli strains K and B. Biochimie 61 501-22 PubMed
  3. Hummel, H et al. (1979) Analysis of lincomycin resistance mutations in Escherichia coli. Mol. Gen. Genet. 169 345-7 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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