rpmJ:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rpmJ

Mnemonic

Ribosomal protein, large

Synonyms

ECK3286, b3299, JW3261, secX[1], secX

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.16 minutes, 74.16 minutes 

MG1655: 3440756..3440640
<gbrowseImage> name=NC_000913:3440640..3440756 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3370639..3370523
<gbrowseImage> name=NC_012967:3370523..3370639 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3327797..3327913
<gbrowseImage> name=NC_012759:3327797..3327913 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4197682..4197798
<gbrowseImage> name=NC_007779:4197682..4197798 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3538501..3538385
<gbrowseImage> name=NC_010473:3538385..3538501 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3440640

Edman degradation

PMID:3542048[2]
PMID:10739205[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrpmJ (Keio:JW3261)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC10457[5]

ΔrpmJ731::kan

PMID:16738554[4]

CGSC:102788


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3261

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAAGTTCGTGCTTCCGTCAA

Primer 2:CCGCCTTGGCGCTGTTTATGCTT

E1H9

Kohara Phage

Genobase

PMID:3038334[7]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[5]

est. P1 cotransduction: 66% [8]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[5]

est. P1 cotransduction: 4% [8]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11232

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11232

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001210

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947805

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1214

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010812

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Kashiwagi, K & Igarashi, K (1987) Nonspecific inhibition of Escherichia coli ornithine decarboxylase by various ribosomal proteins: detection of a new ribosomal protein possessing strong antizyme activity. Biochim. Biophys. Acta 911 180-90 PubMed
  3. Hoving, S et al. (2000) A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal. Chem. 72 1006-14 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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