rplU:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rplU

Mnemonic

Ribosomal protein, large

Synonyms

ECK3175, b3186, JW3153[1], JW3153

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

71.8 minutes 

MG1655: 3331473..3331162
<gbrowseImage> name=NC_000913:3331162..3331473 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3268685..3268374
<gbrowseImage> name=NC_012967:3268374..3268685 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3218310..3218621
<gbrowseImage> name=NC_012759:3218310..3218621 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3333306..3332995
<gbrowseImage> name=NC_007779:3332995..3333306 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3429218..3428907
<gbrowseImage> name=NC_010473:3428907..3429218 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3331162

Edman degradation

PMID:387076[2]
PMID:8740179[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rplU::Tn5KAN-I-SceI (FB21063)

Insertion at nt 21 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21063

contains pKD46

rplU::Tn5KAN-I-SceI (FB21064)

Insertion at nt 20 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21064

does not contain pKD46

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3153

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCTACGCGGTTTTCCAAAGTGG

Primer 2:CCGGCGCTGATGCCAGTAATTTT

14F11

Kohara Phage

Genobase

PMID:3038334[6]

aer-3075::Tn10

Linked marker

CAG12152 = CGSC7438[7]

est. P1 cotransduction: % [8]
Synonyms:zgh-3075::Tn10, zgj-3075::Tn10

sfsB203::Tn10

Linked marker

CAG12072 = CGSC7440[7]

est. P1 cotransduction: 100% [8]
Synonyms:zgj-203::Tn10, zha-203::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG50001

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG50001

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002560

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:949057

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4295

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010470

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Heiland, I & Wittmann-Liebold, B (1979) Amino acid sequence of the ribosomal protein L21 of Escherichia coli. Biochemistry 18 4605-12 PubMed
  3. Pasquali, C et al. (1996) Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database. Electrophoresis 17 547-55 PubMed
  4. 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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