rplR:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rplR

Mnemonic

Ribosomal protein, large

Synonyms

ECK3291, b3304, JW3266[1], JW3266

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.21 minutes 

MG1655: 3443619..3443266
<gbrowseImage> name=NC_000913:3443266..3443619 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3373502..3373149
<gbrowseImage> name=NC_012967:3373149..3373502 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3330423..3330776
<gbrowseImage> name=NC_012759:3330423..3330776 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4194819..4195172
<gbrowseImage> name=NC_007779:4194819..4195172 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3541364..3541011
<gbrowseImage> name=NC_010473:3541011..3541364 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3443266

Edman degradation

PMID:1098937[2]
PMID:10739205[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3266

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGATAAGAAATCTGCTCGTAT

Primer 2:CCGAACTGAAGGCCAGCTTCACG

E1H9

Kohara Phage

Genobase

PMID:3038334[5]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[6]

est. P1 cotransduction: 60% [7]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[6]

est. P1 cotransduction: 5% [7]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10879

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10879

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000870

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947804

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0872

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010825

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Brosius, J et al. (1975) The primary structure of the 5S RNA binding protein L18 from Escherichia coli ribosomes. FEBS Lett. 56 359-61 PubMed
  3. Hoving, S et al. (2000) A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal. Chem. 72 1006-14 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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