rplJ:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rplJ

Mnemonic

Ribosomal protein, large

Synonyms

ECK3976, b3985, JW3948[1], JW3948

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

90.05 minutes 

MG1655: 4178019..4178516
<gbrowseImage> name=NC_000913:4178019..4178516 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4159607..4160104
<gbrowseImage> name=NC_012967:4159607..4160104 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4067699..4068196
<gbrowseImage> name=NC_012759:4067699..4068196 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3456685..3456188
<gbrowseImage> name=NC_007779:3456188..3456685 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4277715..4278212
<gbrowseImage> name=NC_010473:4277715..4278212 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4178022

Edman degradation

PMID:773698[2]
PMID:782920[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3948

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGCTTTAAATCTTCAAGACAA

Primer 2:CCAGCAGCTTCTTTCGCATCGCG

argE86::Tn10

Linked marker

CAG12185 = CGSC7471[5]

est. P1 cotransduction: 38% [6]

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[5]

est. P1 cotransduction: 56% [6]
Synonyms:thi-39::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10871

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10871

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000862

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948490

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0864

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013035

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Pettersson, I et al. (1976) The ribosomal protein L8 is a complex L7/L12 and L10. FEBS Lett. 64 135-8 PubMed
  3. Dovgas, NV et al. (1976) The primary structure of protein L10 from Escherichia coli ribosomes. FEBS Lett. 67 58-61 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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