rplC:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rplC

Mnemonic

Ribosomal protein, large

Synonyms

ECK3307, b3320, JW3282[1], JW3282

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.37 minutes, 74.37 minutes 

MG1655: 3450948..3450319
<gbrowseImage> name=NC_000913:3450319..3450948 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3380831..3380202
<gbrowseImage> name=NC_012967:3380202..3380831 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3337476..3338105
<gbrowseImage> name=NC_012759:3337476..3338105 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4187490..4188119
<gbrowseImage> name=NC_007779:4187490..4188119 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3548693..3548064
<gbrowseImage> name=NC_010473:3548064..3548693 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3450319

Edman degradation

PMID:365579[2]
PMID:9298646[3]
PMID:10739205[4]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rplC in strain TS10-21

Resistant to

rplC(L3) two step tiamulin resistant

PMID:372756[5]

Strain: TS10-21

table 1 and 2

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3282

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCATTGGTTTAGTCGGTAAAAA

Primer 2:CCgGCCTTCACAGCTGGTTTAAC

9H3

Kohara Phage

Genobase

PMID:3038334[7]

E1H9

Kohara Phage

Genobase

PMID:3038334[7]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[8]

est. P1 cotransduction: 45% [9]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[8]

est. P1 cotransduction: 8% [9]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10866

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10866

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000857

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947817

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0859

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010860

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Muranova, TA et al. (1978) The primary structure of ribosomal protein L3 from Escherichia coli 70 S ribosomes. FEBS Lett. 96 301-5 PubMed
  3. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. Hoving, S et al. (2000) A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal. Chem. 72 1006-14 PubMed
  5. Hummel, H et al. (1979) Analysis of lincomycin resistance mutations in Escherichia coli. Mol. Gen. Genet. 169 345-7 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 CGSC: The Coli Genetics Stock Center
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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