rnc:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
rnc |
|---|---|
| Mnemonic |
RNase C |
| Synonyms |
ECK2565, b2567, JW2551, ranA[1], ranA |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
58.22 minutes |
MG1655: 2702085..2701405 |
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NC_012967: 2624875..2625555 |
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NC_012759: 2587210..2587890 |
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W3110 |
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W3110: 2702719..2702039 |
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DH10B: 2793850..2793170 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2701405 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
rnc::Tn5KAN-I-SceI (FB20853) |
Insertion at nt 562 in Plus orientation |
contains pKD46 | |||||
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rnc::Tn5KAN-I-SceI (FB20854) |
Insertion at nt 562 in Plus orientation |
does not contain pKD46 | |||||
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rncG44D |
G44D |
In rnc-105; loss of activity |
seeded from UniProt:P0A7Y0 | ||||
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rnc-105 |
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rnc-40::ΔTn10 |
Auxotrophies |
Presence of A40 insertion mutation causes transcription of era gene derives only from the mini-Tn10 and is Tetracycline dependent. In absence of Tet this insertion prevents era expression. Table 2. |
Strain History W3110 | ||||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2551 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAACCCCATCGTAATTAATCG Primer 2:CCTTCCAGCTCCAGTTTTTTCAA | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 20% [8] | ||
|
Linked marker |
est. P1 cotransduction: 76% [8] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Chen, SM et al. (1990) Expression and characterization of RNase III and Era proteins. Products of the rnc operon of Escherichia coli. J. Biol. Chem. 265 2888-95 PubMed
- ↑ 3.0 3.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Takiff, HE et al. (1992) Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon. J. Bacteriol. 174 1544-53 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
