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Standard Name	puuE
Gene Synonym(s)	ECK1297, b1302, JW1295, goaG^[1], goaG
Product Desc.	4-aminobutyrate aminotransferase^[2]^[3] GABA aminotransferase, putrescine utilization pathway^[4]
Product Synonyms(s)	GABA aminotransferase, PLP-dependent^[1], B1302^[2]^[1], GoaG^[2]^[1], PuuE^[2]^[1] , ECK1297, goaG, JW1295, b1302
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): puuC^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	puuE
Mnemonic	PUtrescine Utilization
Synonyms	ECK1297, b1302, JW1295, goaG^[1], goaG
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	29.39 minutes	MG1655: 1363574..1364839 <gbrowseImage> name=NC_000913:1363574..1364839 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1363918..1365183 <gbrowseImage> name=NC_012967:1363918..1365183 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1254423..1255688 <gbrowseImage> name=NC_012759:1254423..1255688 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1367264..1368529 <gbrowseImage> name=NC_007779:1367264..1368529 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1452970..1454235 <gbrowseImage> name=NC_010473:1452970..1454235 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔpuuE (Keio:JW1295)	deletion	deletion			PMID:16738554	Shigen
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1295	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGCAACAATGAATTCCATCA Primer 2:CCATCGCTCAGCGCATCCTGCAA
1C2	Kohara Phage	Genobase	PMID:3038334
2D3	Kohara Phage	Genobase	PMID:3038334
trpC83::Tn10	Linked marker	CAG18455 = CGSC7371^[5]	est. P1 cotransduction: 13% ^[6] Synonyms:trpB83::Tn10
zcj-233::Tn10	Linked marker	CAG12028 = CGSC7373^[5]	est. P1 cotransduction: 84% ^[6] Synonyms:zci-233::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6646	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13187	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003241	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945446	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2979	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004380	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PuuE
Synonyms	GABA aminotransferase, PLP-dependent^[1], B1302^[2]^[1], GoaG^[2]^[1], PuuE^[2]^[1] , ECK1297, goaG, JW1295, b1302
Product description	4-aminobutyrate aminotransferase^[2]^[3] GABA aminotransferase, putrescine utilization pathway^[4]
EC number (for enzymes)	2.6.1.19^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0030288	outer membrane-bounded periplasmic space				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003867	4-aminobutyrate transaminase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004632	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008615	pyridoxine biosynthetic process				P	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003867	4-aminobutyrate transaminase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.6.1.19	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009448	gamma-aminobutyric acid metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004632	P	Seeded from EcoCyc (v14.0)	complete
	GO:0030170	pyridoxal phosphate binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005814	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030170	pyridoxal phosphate binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015421	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSNNEFHQRR LSATPRGVGV MCNFFAQSAE NATLKDVEGN EYIDFAAGIA VLNTGHRHPD LVAAVEQQLQ QFTHTAYQIV PYESYVTLAE KINALAPVSG QAKTAFFTTG AEAVENAVKI ARAHTGRPGV IAFSGGFHGR TYMTMALTGK VAPYKIGFGP FPGSVYHVPY PSDLHGISTQ DSLDAIERLF KSDIEAKQVA AIIFEPVQGE GGFNVAPKEL VAAIRRLCDE HGIVMIADEV QSGFARTGKL FAMDHYADKP DLMTMAKSLA GGMPLSGVVG NANIMDAPAP GGLGGTYAGN PLAVAAAHAV LNIIDKESLC ERANQLGQRL KNTLIDAKES VPAIAAVRGL GSMIAVEFND PQTGEPSAAI AQKIQQRALA QGLLLLTCGA YGNVIRFLYP LTIPDAQFDA AMKILQDALS D
Length	421
Mol. Wt	44.729 kDa
pI	6.1 (calculated)
Extinction coefficient	17,880 - 18,380 (calc based on 12 Y, 0 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	25..360	PF00202 Aminotransferase class-III		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=puuE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129263	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945446	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004380	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P50457	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6646	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13187	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945446	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003241	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2979	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	14	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	291	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	5^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
puuC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1363554..1363594 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1302 (EcoliWiki Page)	NCBI GEO profiles for puuE
microarray	GenExpDB:b1302 (EcoliWiki Page)	Summary of data for puuE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to puuE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6646	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2979	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1302
EcoGene	EcoGene:EG13187	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003241	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004380	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000027287 (score: 1.000; bootstrap: 98%) ENSANGP00000015566 (score: 0.137)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00037892 (score: 1.000; bootstrap: 97%) WBGene00033756 (score: 0.075)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00020139 (score: 1.000; bootstrap: 95%) WBGene00020382 (score: 0.073)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000027717 (score: 1.000; bootstrap: 95%) ENSCAFP00000016665 (score: 0.116) ENSCAFP00000000346 (score: 0.099)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-050913-100 (score: 1.000; bootstrap: 98%) ZDB-CDNA-050612-172 (score: 1.000; bootstrap: 98%) ZDB-CDNA-040425-2065 (score: 0.117) ZDB-GENE-040426-1133 (score: 0.117) ZDB-CDNA-051127-18 (score: 0.101) ZDB-GENE-051127-33 (score: 0.101)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0037186 (score: 1.000; bootstrap: 95%) FBgn0036381 (score: 0.115)	From Inparanoid:20070104
Drosophila pseudoobscura	GA10859-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000008293 (score: 1.000; bootstrap: 46%)	From Inparanoid:20070104
Mus musculus	MGI:2146052 (score: 1.000; bootstrap: 99%) MGI:1919010 (score: 0.072)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC10397c (score: 1.000; bootstrap: 55%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000160485 (score: 1.000; bootstrap: 100%) NEWSINFRUP00000164668 (score: 0.102) NEWSINFRUP00000155980 (score: 0.100)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00014411001 (score: 1.000; bootstrap: 99%) GSTENP00019899001 (score: 0.111)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000035868 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	GOAG	From SHIGELLACYC
E. coli O157	GOAG	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00202 Aminotransferase class-III
Panther (EcoliWiki Page)	PTHR11986:SF17
Superfamily (EcoliWiki Page)	SUPERFAMILY:53383
EcoCyc	EcoCyc:G6646	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13187	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003241	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2979	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004380	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

puuE:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to puuE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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