purF:Gene
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
<protect>
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
purF |
|---|---|
| Mnemonic |
Purine |
| Synonyms |
ECK2306, b2312, JW2309, adeub, ade(ub)[1], ade, purC |
| edit table |
</protect>
Notes
Location(s) and DNA Sequence
<protect>
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
52.3 minutes |
MG1655: 2428260..2426743 |
||
|
NC_012967: 2373830..2372313 |
||||
|
NC_012759: 2312548..2314065 |
||||
|
W3110 |
|
W3110: 2435684..2434167 |
||
|
DH10B: 2520025..2518508 |
||||
| edit table |
</protect>
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2426746 |
Edman degradation |
| ||
| edit table |
<protect></protect>
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
purF(del) (Keio:JW2309) |
deletion |
deletion |
|||||
|
purF::Tn5KAN-2 (FB20758) |
Insertion at nt 213 in Minus orientation |
does not contain pKD46 | |||||
|
purF::Tn5KAN-2 (FB20759) |
Insertion at nt 213 in Minus orientation |
contains pKD46 | |||||
|
purF1 |
|||||||
|
purF92::Tn10 |
|||||||
|
purF91::Tn10 |
|||||||
|
purF59 |
|||||||
|
purF723(del)::kan |
|||||||
|
purF + |
Resistant to |
Resistance to Nalidixic acid |
Experimental strain: AN2547A |
Figure 1 | |||
|
glnV44 purF-asuC4 |
Insertion of anti-suppression mutation |
Sensitivity to |
Decrease sensitivity to suppressor genes. |
See table 5 for results dealing with anti-suppressor mutations. | |||
| edit table |
<protect></protect>
Notes
Genetic Interactions
<protect>
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2309 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTGCGGTATTGTCGGTATCGC Primer 2:CCTCCTTCGTTATGCATTTCGAG | |
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 21% [10] | ||
|
zfd-1::Tn10 |
Linked marker |
est. P1 cotransduction: 3% [10] | |
| edit table |
<protect></protect>
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
<protect></protect>
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Tso, JY et al. (1982) Glutamine phosphoribosylpyrophosphate amidotransferase from cloned Escherichia coli purF. NH2-terminal amino acid sequence, identification of the glutamine site, and trace metal analysis. J. Biol. Chem. 257 3532-6 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Helling, RB & Adams, BS (1970) Nalidixic acid-resistant auxotrophs of Escherichia coli. J. Bacteriol. 104 1027-9 PubMed
- ↑ Sullivan, MA & Bock, RM (1985) Isolation and characterization of antisuppressor mutations in Escherichia coli. J. Bacteriol. 161 377-84 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
