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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	proP
Gene Synonym(s)	ECK4104, b4111, JW4072^[1], JW4072
Product Desc.	ProP proline/betaine MFS transporter^[2]^[3] Proline/betaine permease, minor; osmosensor/osmoregulator^[4]
Product Synonyms(s)	proline/glycine betaine transporter^[1], B4111^[2]^[1], ProP^[2]^[1] , ECK4104, JW4072, b4111
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): proP^[2], OP00211
Regulation/Activity
Quick Links
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Notes

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Mg(2+) stimulon-PhoPQ regulon, EvgAS-PhoPQ cascade enhanced-expression subset. Major Facilitator Superfamily (MFS).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	proP
Mnemonic	Proline
Synonyms	ECK4104, b4111, JW4072^[1], JW4072
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	93.29 minutes	MG1655: 4328525..4330027 <gbrowseImage> name=NC_000913:4328525..4330027 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4309216..4310718 <gbrowseImage> name=NC_012967:4309216..4310718 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4267260..4268762 <gbrowseImage> name=NC_012759:4267260..4268762 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4335180..4336682 <gbrowseImage> name=NC_007779:4335180..4336682 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4428887..4430389 <gbrowseImage> name=NC_010473:4428887..4430389 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
ΔproP (Keio:JW4072)	deletion	deletion			PMID:16738554	Shigen CGSC10930^[5]
ΔproP737::kan					PMID:16738554	CGSC:102472
proP220::Tn5			Auxotrophies	Proline Auxotroph	PMID:6355059	Strains: WG170 and WG174
proP219			Resistant to	Resistant to Dehydroproline	PMID:6355059	Strain: WG170
proP220::Tn5			Resistant to	Resistant to Dehydroproline	PMID:6355059	Strain: WG171
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4072	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCTGAAAAGGAAAAAAGTAAA Primer 2:CCTTCATCAATTCGCGGATGTTG
2C12	Kohara Phage	Genobase	PMID:3038334
8H1	Kohara Phage	Genobase	PMID:3038334
zje-2241::Tn10	Linked marker	CAG18427 = CGSC7481^[5]	est. P1 cotransduction: 43% ^[6]
cadB2231::Tn10	Linked marker	CAG18488 = CGSC7479^[5]	est. P1 cotransduction: 34% ^[6] Synonyms:zjd-2231::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11612	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11612	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001560	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948626	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1569	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013462	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	ProP
Synonyms	proline/glycine betaine transporter^[1], B4111^[2]^[1], ProP^[2]^[1] , ECK4104, JW4072, b4111
Product description	ProP proline/betaine MFS transporter^[2]^[3] Proline/betaine permease, minor; osmosensor/osmoregulator^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0016021	integral to membrane	PMID:10026245	IDA: Inferred from Direct Assay		C		complete
	GO:0005886	plasma membrane	PMID:10026245	IDA: Inferred from Direct Assay		C		complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004736	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005828	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0055085	transmembrane transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005828	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055085	transmembrane transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005829	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006865	amino acid transport	PMID:10026245	IDA: Inferred from Direct Assay		P		complete
	GO:0006865	amino acid transport	PMID:10026245	IDA: Inferred from Direct Assay		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Proline transporter activity is sensitive to the osmolality of the growth medium. Activation and polar localization depend on cardiolipin content of the membrane.

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 12 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
Pole		Fluorescence microscopy PMID:17504273 PMID:18326496
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MLKRKKVKPI TLRDVTIIDD GKLRKAITAA SLGNAMEWFD FGVYGFVAYA LGKVFFPGAD PSVQMVAALA TFSVPFLIRP LGGLFFGMLG DKYGRQKILA ITIVIMSIST FCIGLIPSYD TIGIWAPILL LICKMAQGFS VGGEYTGASI FVAEYSPDRK RGFMGSWLDF GSIAGFVLGA GVVVLISTIV GEANFLDWGW RIPFFIALPL GIIGLYLRHA LEETPAFQQH VDKLEQGDRE GLQDGPKVSF KEIATKYWRS LLTCIGLVIA TNVTYYMLLT YMPSYLSHNL HYSEDHGVLI IIAIMIGMLF VQPVMGLLSD RFGRRPFVLL GSVALFVLAI PAFILINSNV IGLIFAGLLM LAVILNCFTG VMASTLPAMF PTHIRYSALA AAFNISVLVA GLTPTLAAWL VESSQNLMMP AYYLMVVAVV GLITGVTMKE TANRPLKGAT PAASDIQEAK EILVEHYDNI EQKIDDIDHE IADLQAKRTR LVQQHPRIDE
Length	500
Mol. Wt	54.848 kDa
pI	7.2 (calculated)
Extinction coefficient	63,830 - 64,330 (calc based on 17 Y, 7 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	25..453	PF00083 Sugar (and other) transporter	PMID:19920124
Domain	454..499	PF08946 Osmosensory transporter coiled coil	PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=proP taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131937	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948626	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013462	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0C0L7	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11612	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11612	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948626	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001560	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1569	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	3.39E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	465	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	613	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	826	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
proP
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4328505..4328545 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4111 (EcoliWiki Page)	NCBI GEO profiles for proP
microarray	GenExpDB:b4111 (EcoliWiki Page)	Summary of data for proP from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to proP Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11612	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1569	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4111
EcoGene	EcoGene:EG11612	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001560	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013462	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Caenorhabditis briggsae	WBGene00039162 (score: 1.000; bootstrap: 100%) WBGene00027098 (score: 0.160) WBGene00024393 (score: 0.149) WBGene00027474 (score: 0.140) WBGene00025704 (score: 0.139) WBGene00024395 (score: 0.135) WBGene00032394 (score: 0.133) WBGene00024452 (score: 0.120) WBGene00042042 (score: 0.119) WBGene00030924 (score: 0.116) WBGene00039573 (score: 0.116) WBGene00026048 (score: 0.110) WBGene00024396 (score: 0.109) WBGene00027974 (score: 0.109) WBGene00027345 (score: 0.105) WBGene00032396 (score: 0.104) WBGene00029653 (score: 0.104) WBGene00032547 (score: 0.097) WBGene00031157 (score: 0.081) WBGene00041186 (score: 0.080) WBGene00038285 (score: 0.076) WBGene00039083 (score: 0.074) WBGene00037407 (score: 0.072) WBGene00041731 (score: 0.070) WBGene00033157 (score: 0.066) WBGene00024723 (score: 0.064) WBGene00039084 (score: 0.056) WBGene00024312 (score: 0.053) WBGene00039082 (score: 0.052)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00020906 (score: 1.000; bootstrap: 55%) WBGene00010646 (score: 0.188) WBGene00008677 (score: 0.154) WBGene00007669 (score: 0.149) WBGene00012443 (score: 0.136) WBGene00018268 (score: 0.123) WBGene00012085 (score: 0.121) WBGene00020584 (score: 0.121) WBGene00016003 (score: 0.120) WBGene00019187 (score: 0.115) WBGene00010931 (score: 0.108) WBGene00011185 (score: 0.106) WBGene00008647 (score: 0.090) WBGene00007566 (score: 0.084) WBGene00021028 (score: 0.081) WBGene00019655 (score: 0.077) WBGene00020698 (score: 0.076) WBGene00020700 (score: 0.068) WBGene00020157 (score: 0.067) WBGene00020697 (score: 0.059) WBGene00007947 (score: 0.057) WBGene00013088 (score: 0.051) WBGene00021158 (score: 0.050)	From Inparanoid:20070104
Homo sapiens	ENSP00000342335 (score: 1.000; bootstrap: 100%) ENSP00000321735 (score: 0.532) ENSP00000261187 (score: 0.411) ENSP00000285753 (score: 0.376) ENSP00000330141 (score: 0.305) ENSP00000310490 (score: 0.227) ENSP00000343022 (score: 0.227) ENSP00000309751 (score: 0.212) ENSP00000319991 (score: 0.191) ENSP00000307169 (score: 0.153) ENSP00000315439 (score: 0.149) ENSP00000276033 (score: 0.145) ENSP00000355327 (score: 0.090)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000013232 (score: 1.000; bootstrap: 100%) ENSMMUP00000008120 (score: 0.375) ENSMMUP00000019399 (score: 0.324) ENSMMUP00000004803 (score: 0.303) ENSMMUP00000003630 (score: 0.280) ENSMMUP00000027882 (score: 0.212) ENSMMUP00000023079 (score: 0.198) ENSMMUP00000027884 (score: 0.151) ENSMMUP00000019690 (score: 0.129) ENSMMUP00000014523 (score: 0.129) ENSMMUP00000028359 (score: 0.113) ENSMMUP00000014111 (score: 0.093) ENSMMUP00000017292 (score: 0.084)	From Inparanoid:20070104
Mus musculus	MGI:1933438 (score: 1.000; bootstrap: 100%) MGI:1929519 (score: 0.537) MGI:1330284 (score: 0.392) MGI:106013 (score: 0.372) MGI:2443515 (score: 0.292) MGI:2144585 (score: 0.248) MGI:2147716 (score: 0.235) MGI:1916559 (score: 0.189) MGI:2385183 (score: 0.154) MGI:1203732 (score: 0.140) MGI:2663709 (score: 0.117) MGI:1919722 (score: 0.082)	From Inparanoid:20070104
Shigella flexneri	PROP	From SHIGELLACYC
E. coli O157	PROP	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:103473
Superfamily (EcoliWiki Page)	SUPERFAMILY:103661
Pfam (EcoliWiki Page)	PF00083 Sugar (and other) transporter
Pfam (EcoliWiki Page)	PF08946 Osmosensory transporter coiled coil
EcoCyc	EcoCyc:EG11612	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11612	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001560	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1569	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013462	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

proP:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to proP Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

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