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Gene |

Product(s) |

Expression|

Evolution |

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Standard Name	pptA
Gene Synonym(s)	ydcE, ECK1455, b1461, JW1456^[1]^[2], JW1456
Product Desc.	probable 4-oxalocrotonate tautomerase (4-OT)^[2]^[3]; Component of probable 4-oxalocrotonate tautomerase (4-OT)^[3] Phenylpyruvate tautomerase; other substrates are (p-hydroxyphenyl)pyruvate and 2-hydroxy-2,4-pentadienoate; homodimeric^[4]
Product Synonyms(s)	4-oxalocrotonate tautomerase^[1], PptA^[2]^[1], YdcE^[2]^[1], B1461^[2]^[1] , ECK1455, JW1456, ydcE, b1461
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ydcE^[2]
Regulation/Activity
Quick Links
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Notes

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Cleavage of PptA fMet was confirmed by mass spec: obs. MW = 8540. N-terminal proline is presumed to be the catalytic base residue of the active site.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	pptA
Mnemonic	PhenylPyruvate Tautomerase
Synonyms	ydcE, ECK1455, b1461, JW1456^[1]^[2], JW1456
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	33 minutes	MG1655: 1531076..1531309 <gbrowseImage> name=NC_000913:1531076..1531309 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1507202..1507429 <gbrowseImage> name=NC_012967:1507202..1507429 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1423135..1423368 <gbrowseImage> name=NC_012759:1423135..1423368 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1534766..1534999 <gbrowseImage> name=NC_007779:1534766..1534999 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1621671..1621904 <gbrowseImage> name=NC_010473:1621671..1621904 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource

Resource Type

Source

Notes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11761	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11761	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001697	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945731	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1711	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004875	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YdcE
Synonyms	4-oxalocrotonate tautomerase^[1], PptA^[2]^[1], YdcE^[2]^[1], B1461^[2]^[1] , ECK1455, JW1456, ydcE, b1461
Product description	probable 4-oxalocrotonate tautomerase (4-OT)^[2]^[3]; Component of probable 4-oxalocrotonate tautomerase (4-OT)^[3] Phenylpyruvate tautomerase; other substrates are (p-hydroxyphenyl)pyruvate and 2-hydroxy-2,4-pentadienoate; homodimeric^[4]
EC number (for enzymes)	5.3.2.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00718	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017284	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006725	cellular aromatic compound metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004370	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016853	isomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004370	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016853	isomerase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0413	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016862	intramolecular oxidoreductase activity, interconverting keto- and enol-groups	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00718	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016862	intramolecular oxidoreductase activity, interconverting keto- and enol-groups	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017284	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of probable 4-oxalocrotonate tautomerase (4-OT)	could be indirect
Protein	yhcA		PMID:16606699	Experiment(s):EBI-1139876
Protein	glgB		PMID:16606699	Experiment(s):EBI-1139876
Protein	pyrB		PMID:16606699	Experiment(s):EBI-1139876
Protein	narZ		PMID:16606699	Experiment(s):EBI-1139876
Protein	ydeP		PMID:16606699	Experiment(s):EBI-1139876
Protein	yfaS		PMID:16606699	Experiment(s):EBI-1139876
Protein	lacZ		PMID:16606699	Experiment(s):EBI-1139876
Protein	yggF		PMID:16606699	Experiment(s):EBI-1139876
Protein	nadE		PMID:16606699	Experiment(s):EBI-1139876
Protein	ybiB		PMID:16606699	Experiment(s):EBI-1139876
Protein	yehQ		PMID:16606699	Experiment(s):EBI-1139876
Protein	yqhH		PMID:16606699	Experiment(s):EBI-1139876
Protein	yqgC		PMID:16606699	Experiment(s):EBI-1139876
Protein	groL		PMID:16606699	Experiment(s):EBI-1139876
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MPHIDIKCFP RELDEQQKAA LAADITDVII RHLNSKDSSI SIALQQIQPE SWQAIWDAEI APQMEALIKK PGYSMNA
Length	77
Mol. Wt	8.672 kDa
pI	4.8 (calculated)
Extinction coefficient	12,490 - 12,615 (calc based on 1 Y, 2 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P31992
Domain	2..55	PF01361 Tautomerase enzyme		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pptA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129420	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945731	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004875	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P31992	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11761	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11761	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945731	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001697	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1711	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	323	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	924	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	220^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ydcE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1531056..1531096 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1461 (EcoliWiki Page)	NCBI GEO profiles for pptA
microarray	GenExpDB:b1461 (EcoliWiki Page)	Summary of data for pptA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to pptA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11761	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1711	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1461
EcoGene	EcoGene:EG11761	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001697	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004875	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:55331
Pfam (EcoliWiki Page)	PF01361 Tautomerase enzyme
EcoCyc	EcoCyc:EG11761	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11761	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001697	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1711	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004875	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.

pptA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to pptA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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